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Valuable Information About the Iceman
Objective Lens for Advanced Life Science Imaging
Olympus has introduced the new PLAPON 60xOSC objective lens with an NA of 1.4, offering outstanding levels of chromatic aberration correction for life science imaging applications. This super-corrected objective is designed for excellent fluorescence imaging down to an unprecedented 405nm. It is therefore ideal for use in cell biology and neuroscience, as well as any applications requiring lasers for inter- and intra-cellular imaging of biological specimens.
JPK Instruments reports on the work of the Stark research group at LMU-Munich in cooperation with the EURAC Bolzano where a NanoWizard®
AFM system has been used to
reveal the nanostructure and mechanics of mummified type I collagen from the 5300- year-old Tyrolean Iceman.
In the Department of Earth and Environmental Sciences at the Ludwig-Maximilians University in Munich, scientists work on nanoscale biomaterial characterisation. Their efforts have recently been showcased in the study of mummified skin from the 5300-year-old Tyrolean Iceman. By way of background, skin protects the body from pathogens and degradation. Even mummified skin retains this function. The action of micro-organisms or other external influences may degrade the connective tissue and lay the subjacent tissue open. To determine the degree of tissue preservation in mummified human skin and the reason for its durability, PhD-student Marek Janko of Professor Robert Stark’s research group has investigated the structural integrity of its main protein, type I collagen using an atomic for ce microscope, the NanoWizard®
II, from JPK Instruments.
Janko’s research focuses on the study of mechanical properties of nanobio materials. Use of AFM and Raman spectroscopy are non- invasive techniques and samples may be re- analysed many times. The choice of the NanoWizard for this work is two-fold. The combination of AFM with optical microscopy enables normal histology tissue samples, 2-4 microns thick and relatively ‘rough’ to be readily studied in ambient conditions. The optical microscope enable identification of areas for study and then the AFM is used to effectively zoom in to study individual fibrils on the tens of nanometer scale. Being able to zoom to and study a single fibril just 30-40nm in height means the individual repeat units of 67nm may be identified and probed. This enables nanoindentation studies to be carried employing force-versus-distance measurements to measure mechanical properties and in particular, Young’s modulus.
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The PLAPON 60xOSC lens provides 0.1-0.2µm of chromatic aberration when working with lasers from 405 to 650nm, exceeding the optimal performance of comparable objectives. Since 405nm light is used for a wide range of scientific imaging applications, this advanced chromatic aberration enables users to perform multicolour imaging with the highest possible colour fidelity, even at shorter wavelengths. Reliable co-localisation and 3-dimensional microstructure studies can be successfully carried-out. Providing an advanced optical performance with outstanding flatness, even fine lines are clearly defined, significantly reducing the occurrence of shadows at the periphery of the image. As a result, users obtain bright, crisp images from edge to edge. In addition, the PLAPON 60x objective provides excellent near-infrared fluorescence imaging up to 850nm, with a significant reduction in chromatic and other aberration types. Olympus also guarantees to meet all optical performance specifications provided.With a working distance of 0.12mm and a field number of 22, the new Olympus PLAPON x60 objective incorporates proprietary Olympus technology to enhance point spread function for confocal imaging at low ultraviolet wavelengths. With enhanced flatness and improved brightness at the periphery of the field the objective is designed for use with an appropriate cover glass and immersion oil. Furthermore, it can also be adapted for use with differential interference contrast (DIC).
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Chemical Imaging Analysis Software Available to License
Rapidly becoming the industry standard for analysing chemical imaging data, ISys 5.0 from Malvern Instruments is available for license on a wide range of analytical systems. Providing a single platform that supports the import and easy manipulation of MIR, NIR and Raman imaging and mapping data files from a variety of vendors, ISys includes all the tools needed to intuitively understand chemical imaging data, in spectral (chemical) and spatial (image) dimensions simultaneously. ISys is available in both 64-bit and 32-bit editions, and a 30-day trial version can be downloaded free of charge from the Malvern website.
Able to run advanced functionality on most well known platforms without special preparation, ISys can handle direct data imports from Kaiser, Horiba Jobin-Yvon, Renishaw, Witech, Varian, Thermo, Bruker, PerkinElmer, and Chemimage mapping and imaging platforms. Files in generic formats such as Grams, Matlab, ENVI, Metamorph, multi-page tiff and ASCII, can also be accessed. Other non- vibrational spectroscopic imaging data platforms can also be imported - EDAX X-ray fluorescence and Horiba Jobin Yvon XGT file formats, for example.
ISys turns images to answers for a variety of chemical imaging techniques. It delivers reliable cross- platform compatibility at speeds that enable researchers to work effectively with the huge data sets and image files associated with MIR, NIR and Raman imaging and chemical mapping. Powerful and flexible, ISys is ideal for processing both routine and complex statistical analyses, pattern recognition algorithms, chemometric experiments, and domain (particle) analyses.
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