9
New Columns Accelerate LC/MS Analysis of Synthetic Oligonucleotides Phenomenex, Inc introduces Clarity®
Oligo-MS™ columns for rapid and efficient LC/MS characterisation and quality control of synthetic RNA and DNA.
Based on the company’s core-shell particle technology, the new C18 columns deliver significantly shorter run times than traditional media, along with increased resolution and sensitivity. With the high resolving power of Clarity Oligo-MS, impurities in complex synthetic mixtures can be separated from the peak of interest in less than 10 minutes. These new Oligo-MS columns, which are part of the Clarity BioSolutions portfolio, are packed with either 2.6µm or 1.7µm particles, enabling high efficiency on any HPLC or UHPLC platform. The Clarity Oligo-MS 2.6µm columns operate at reduced backpressures compared to other oligonucleotide-specific columns, delivering UHPLC performance on any LC instrument, and methods are easily transferable. The Clarity Oligo-MS 1.7µm columns boost the performance of existing sub-2µm methods on high-pressure systems.
“LC/MS is the analysis technique of choice in synthetic oligo quality control as well as for the characterisation of oligo-based therapeutics and their metabolites,” explained Cathy Cordova, Marketing Manager for Phenomenex. “With our new core-shell particle technology, we bring significant improvements to this process in the areas of sample throughput and MS sensitivity.” With traditional, fully porous particles, efficiency decreases as flow rate increases, resulting in loss of resolution and sensitivity and longer overall analysis time. The Phenomenex core-shell technology enables high resolution and sensitivity over an extended linear velocity without generating excessive backpressure.
Circle no. 9 New Applications to Market-Leading GPC/SEC System
Malvern Instruments has extended the applications for its Viscotek Triple Detector Array (TDA), the multi-detector platform for gel permeation/size exclusion chromatography (GPC/SEC), through the inclusion of a new viscometer in the setup. The TDA maximises the productivity of GPC/SEC analysis by simultaneously measuring absolute molecular weight, molecular size and intrinsic viscosity. The new viscometer combines unrivalled sensitivity with an innovative 316 stainless steel transducer construction that offers excellent chemical resistance. It therefore provides the most precise intrinsic viscosity and structural information for an expanded range of biomolecules, natural and synthetic polymers. The unique viscometer design uses digital inert transducer technology to give a faster, more sensitive response to viscosity changes than traditional transducers. Its 316 stainless steel construction means that there are now few limitations, in terms of salt content or pH, on the chromatographic conditions that can be used. This is a significant benefit for protein analysis. The use of inert capillaries in the measuring flow path is also advantageous for many biomolecule applications.
The TDA lies at the heart of the Viscotek TDAmax: a complete, advanced GPC/SEC system suitable for all macromolecular applications. However, each element of the TDA can be purchased alone or in combination, offering exceptional flexibility to those with existing GPC/SEC systems. UV and/or PDA detectors are also available to further augment detection capabilities.
Circle no. 10
NEW RANGE Biochrom Libra – real choice in UV-visible spectroscopy
Easy to choose and easytouse
Range includes double beam and variable bandwidth models
Stand alone or PC driven with flexible application-led software
www.biochrom.co.uk www.biochrom-us.com
Circle no. 11
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