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VIALS & ACCESSORIES
Effective Cryopreservation of iPS Cells Stem Cellbanker®
well as other valuable cells.
In a recent study to determine the best cryopreservation solution to maintain induced pluripotent stem (iPS) cells, a team of researchers from the Okayama University Graduate School of Medicine (Japan) compared 12 kinds of commercially prepared cryopreservation solutions and found that Stem Cellbanker outperformed the other 11 solutions. The researchers concluded that Stem Cellbanker showed the highest cell viability and proliferation of all the solutions tested and can be widely used as it does not require any special skills to use.
Co-author of the study - Hirofumi Noguchi, MD, PhD, Department of Gastroenterological Surgery, Transplant and Surgical Oncology at the Okayama University Graduate School of Medicine commented "iPS cells are a promising alternative to embryonic stem cells and can be used in place of bone marrow cells, stromal cells and adipose tissue-derived stem cells". He added "However, the viability of human iPS cells, like embryonic stem cells, decreases significantly during cryopreservation. A wide variety of cryopreservation solutions have been used, however many are toxic or ineffective for use in extended cryopreservation."
Supplied ready-to-use with a simple usage protocol, Stem Cellbanker is completely free of serum and animal derived components, and contains only Japanese, European or US Pharmacopoeia graded ingredients. Stem Cellbanker significantly increases cell viability while maintaining cell pluripotency, normal karyotype and proliferation ability following resuscitation from cryopreservation, even after extended long-term storage. Independent research has demonstrated that hESC, MSCs and iPS cells cryopreserved with Stem Cellbanker produce significantly higher cell viability (> 90%) over conventional freezing medium, while retaining cell pluripotency, normal karyotype and proliferation ability. Every batch of Stem Cellbanker cryopreservation medium is performance tested on Jurkat and SK-007 cells. Additional standard evaluations for endotoxins, pH, osmolarity and Mycoplasma contaminants are undertaken to ensure GMP equivalent quality.
MORE INFO. 185
from Amsbio is a chemically defined freezing medium with optimised formulation for stem cells and iPS cells storage as
Automated Salmonella Test Gains International Approval bioMérieux have announced that its VIDAS®
Fast and Flexible PCR Thermal Cycler for Complex Applications
UP Salmonella (SPT), an automated test for the detection of
Salmonella species, has been granted Official Methods of Analysis (OMA) approval by AOAC International on a wide variety of food products and environmental samples.
The scope of this approval also includes 375g samples. This level of certification assures VIDAS UP Salmonella users that the test meets stringent AOAC standards for excellence in pathogen detection.
VIDAS UP Salmonella boasts an extremely simplified protocol to help streamline the workflow of the food- testing laboratory. The VIDAS UP Salmonella enables significant savings in media, ancillary materials and labour time, while providing results in as little as 19 hours.
“This recognition is a result of bioMérieux’s ongoing commitment in providing rapid pathogen detection methods to agri-food industries,” said Jean-Marc Durano, Corporate Vice President, Industrial Microbiology Unit. Our solutions are designed to help food manufacturers and processors ensure the safety of their food products.”
MORE INFO. 186
Analytik Jena introduces the FlexCycler2, a modern thermal cycler of Analytik Jena, which combines exceptional design with reliable technology in one system. By the Quick-X-Change block exchange system block modules can be exchanged within seconds and the instrument adapted flexibly to changing requirements. For this purpose, in total six different mono and twin block modules are available that can replace each other.
The two independent blocks of the twin block modules allow the simultaneous run of two different PCR programs and thereby help to avoid bottlenecks. The 96 well block module and the 48 well twin block module for optimisation of new primer pairs are optionally available with gradient function. The FlexCycler2 provides state-of-the-art heating and cooling rates and reproducible conditions in all positions of the sample blocks with its excellent temperature uniformity. The user-friendly software concept in combination with extensive additional software options makes the FlexCycler2 the perfect system for challenging PCR applications.
MORE INFO. 187
INTERNATIONAL LABMATE - APRIL 2013
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