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DNA Test Identifies Eight Animal Species in Foodstuffs Quickly and Reliably
With horsemeat in lasagnes and pork in kebabs, the meat scandal is escalating day by day. Consumer protection groups and politicians are now calling for Europe-wide DNA testing for a whole range of foodstuffs.
Using the CarnoCheck DNA test, hundreds of samples are currently being tested at various European laboratories as part of efforts to find answers in the horsemeat la-belling fraud. Greiner Bio-One has been offering the CarnoCheck DNA test kit since 2004, enabling eight animal species to be detected in foodstuffs quickly and reliably. As well as horses, the kit covers pigs, donkeys, sheep, cattle, chickens, turkeys and goats.
Greiner Bio-One, based in Frickenhausen, specialises in developing molecular biol-ogy analysis methods. Using ‘DNA chip technology’, which is already deployed by countless laboratories, the specific genetic fingerprint of many different parameters can be detected unambiguously in one single analysis. With the CarnoCheck DNA chip, the eight animal species can be identified in foodstuffs down to a detection limit of 0.5%. The results are available within three hours. A comprehensive control sys-tem also ensures the quality of the analysis.
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First Working Prototype of Unique Handheld System for on-the-spot Diagnostic Results Unveiled
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AgPlus Diagnostics is pleased to announce that the first functional prototype of the handheld AgPlus Diagnostics analytical platform is now fully operational and available for demonstration. Delivering laboratory-grade, quantifiable results within just 10 minutes of sample application the portable AgPlus technology system has enormous potential in a wide variety of applications where a rapid, accurate and sensitive result is important for fast and reliable decision making.
The AgPlus Diagnostics platform utilises electrochemical immunoassay technology in a patented microfluidic chip format.
The unique signalling and measurement system, based on the detection of amplified silver ions by anodic stripping voltammetry, allows extremely sensitive, accurate and quantifiable results to be obtained from a very small sample volume (50µl) within minutes.
The system does not rely on an optical based detection method, so no sample preparation is required, making it ideal for multiple sample types and for testing in a range of settings.
The first fully functional AgPlus technology prototype will demonstrate an assay for human Troponin I, a cardiac marker used in clinical settings. The AgPlus Troponin I assay is currently showing a dynamic range of .1ng/ml – 50ng/ml with CVs <20% in a non-optimised format with assay run times of 10 minutes.
In addition, AgPlus tests for cortisol and testosterone are currently in development, which will be of value in sports science applications for developing more tailored training plans for athletes and to help reduce chances of Over Training Syndrome.
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The technology has been developed so that it can be applied to new and established chemistries and the platform software is easily integrated into existing information management systems. AgPlus Diagnostics foresee further potential for this technology in human (clinical and non-clinical) and veterinary diagnostics, as well as in environmental and military applications.
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“The first working AgPlus technology system prototype is an important milestone for AgPlus Diagnostics, demonstrating the successful coming together of this novel diagnostic assay and its unique analytical platform to achieve a fully functional, portable system that can deliver accuracy and sensitivity that’s on a par with laboratory analysers,” commented CEO, Keith Page.
AgPlus Diagnostics is seeking to develop partnerships and licensing agreements with collaborators in a variety of applications and business sectors.
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INTERNATIONAL LABMATE - APRIL 2013
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