Chromatography 13 New Chromatography Consumables Exhibited at Pittcon 2013
Thermo Fisher Scientific Inc have announced that it featured a number of new chromatography consumables within booth 1835 during Pittcon 2013. Among them are several columns designed for improved analysis of chemical compounds. The Company has added five phases to its family of Thermo Scientific Accucore solid core HPLC columns: Accucore Polar Premium, a rugged amide-embedded C18 phase offering complementary selectivity to conventional C18 columns; Accucore Phenyl-X, featuring a proprietary reversed-phase shape selectivity with high aromatic selectivity; Accucore C30, designed for high shape selectivity for hydrophobic, long chain, structurally related isomers; Accucore Urea-HILIC for HILIC, designed for selectivity and low ion exchange activity; Accucore 150-Amide-HILIC, designed to separate hydrophilic biomolecules in HILIC mode, intended for glycan separations.
Also new to the portfolio are Thermo Scientific Acclaim SEC-300 and SEC-1000 size exclusion HPLC columns designed for high-resolution separation of water soluble polymers and oligomers. They are optimised for determining molecular weight distribution and characterisation of water soluble polymers used in a wide range of products found in pharmaceuticals, cosmetics, food and industrial processes. The columns are intended for research use only. For high-resolution and high-throughput trace LC-MS analysis of the herbicides diquat and paraquat in plants, Thermo Fisher Scientific features the Acclaim Trinity Q1 HPLC column. Tests have demonstrated that the new column delivers the retention, resolution, peak shape and speed needed to improve diquat and paraquat analysis in common terrestrial and aquatic plant matrixes, without the need for pairing reagents or exotic mobile phases. Also launching at Pittcon is the Thermo Scientific National SureStop vial, designed to eliminate variations in sealing inherent in manual tightening, while optimising septum compression each time. The threads contain a ‘stop’ designed to prevent under- and over-tightening. Evaporation studies have shown that the SureStop vial produces the lowest loss to evaporation and the lowest standard deviation in evaporation of any mainstream 2mL vial. This can protect data quality by reducing a source of experimental variation.
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EMBL Hamburg Researchers use Multi-Detector array SEC System for Enhanced Protein Characterisation by SAXS
Researchers at the European Molecular Biology Laboratory (EMBL) in Hamburg, Germany, have incorporated a multi- detector array size exclusion chromatography (SEC) system from Malvern Instruments (Viscotek TDA –Triple Detector Array) at the laboratory’s powerful small angle X-ray scattering (SAXS) beamline to improve the productivity and quality of detailed protein characterisation work.
Use of the Viscotek TDA triple detector array system prior to analysis provides complementary biophysical data and simultaneously purifies samples for measurement. This results in more information and better quality data from each experiment. This innovative set-up is not only limited to the institute itself, but is widely accessible to structural biology researchers via BioStructX, a European Commission initiative that creates a single gateway to Europe’s leading synchrotron facilities.
SEC produces a fractionated sample for measurement, separated on the basis of hydrodynamic size. The Viscotek TDA incorporates refractive index, UV and light scattering detectors which in combination provide concentration and absolute molecular weight measurement for these eluting fractions. Integrating this system with the SAXS beamline provides it with purified samples for measurement that give enhanced signal-to-noise ratios and high quality data. In combination with the biophysical information from the TDA these data provide detailed information about the internal shape and structure of proteins that advances understanding of their behaviour. In particular SAXS data is valued for the determination and monitoring of the low resolution structures and conformational changes that proteins exhibit.
Both Malvern Instruments and EMBL Hamburg are partners in the BioStructX project which brings together facilities from across Europe to establish a state-of-the-art coordinated and multi-site infrastructure to support access for key methods in structural biology. The new integrated SEC-SAXS set-up was developed by a number of experts within EMBL, and with input from Malvern Instruments to enhance the department’s offering to BioSAXS users transnationally. The automated multi-pronged characterisation it offers has already demonstrated its significant advantages to the first users and is expected to be in considerable demand as the BioSructX project advances.
Researchers interested in using the EMBL facility can find more information on the BioSAXS webpages. MORE INFO. 36
Someone needs Release of a New Control Driver for ELSD System
DataApex is pleased to announce the release of a new control driver for the Evaporative Light-Scattering Detector 90LT, manufactured by Sedere Company. This is already the 3rd Sedere detector its control and digital data acquisition has been incorporated into Clarity Chromatography SW. The driver for ELSD 90LT has been officially released with Clarity Chromatography Software version 4.0.3.
Sedere is a leading manufacturer of Low-Temperature Evaporative Light- Scattering Detectors. Sedere has more than 25 years of experience in the development of versatile detection systems for all chromatography applications and their detectors are distributed worldwide.
Clarity Chromatography Software has a strong position in the chromatography data systems market. Clarity, the third generation of DataApex products, allows controlling more than 400 different instruments from the single environment and offers its users very high flexibility. Clarity is highly regarded for its intuitive approach, excellent performance, cost-effectiveness and proficient technical support.
DataApex is solely focused on chromatography software development. A strong emphasis is placed on technological innovation, visionary adoption of new laboratory standards, best practices and extensive customer support. DataApex products are sold in over 80 countries around the world. Ten chromatography instrument manufacturers privately resell labelled versions of DataApex’s software.
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a MiniVap™ Of course you wouldn’t use a hair dryer to evaporate chromatography samples in a single microplate, but you might well be fed up with queuing to use a large departmental evaporator for the same purpose. In which case, you need a Porvair MiniVap. It’s small, quick, flexible and won’t damage your samples – a truly individual evaporator, in fact. Go to
www.porvair-sciences.com/ for more information.
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INTERNATIONAL LABMATE - APRIL 2013
Now with HPLC Vial Holder
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