ACTIVES
(from laboratory methodology 0.5 kg) or 0.1% PGFE. All results shown have been calculated relative to the senescent fibroblast culture control group (P17- NHDF). The MMP-1 production of the non- senescent positive control (P7-NHDF) was 34%, that of the TGF-β treated cells 61% of the control group. All three cell treatments involving PGFE (PGFE/SAP SU, PGFE/SAP L and PGFE) effected production of MMP-1 to be lowered to 80% of the control group's production. In an identically designed experiment
carried out six months previously, laboratory scale-preparations of PGFE extract and PGFE/SAP were tested (results not shown). Both treatments also led to a reduced MMP-1 synthesis of 80%.
In vivo After successfully passing safety- and stability-tests, a cream formulation containing 1% PGFE/SAP (see Table 1) was used in two in vivo studies each with different measurement parameters. In Study 1 skin density was determined on the volar forearms and high-resolution photos of the face were analysed. In Study 2 the depth of wrinkles in the crow’s feet area around the lateral corner of the eye was determined and likewise high- resolution facial photos were taken.
Figure 8: High-resolution photographs of the lateral area around the eye (crow’s feet area).
Skin density measurement using ultrasonography
The skin density on the volar forearm was determined using ultrasonography. Measurements were performed before (D0), during (D28) and at the end of treatment (D56) with a cream formulation containing 1% PGFE/SAP. An average increase in skin density of 32.5% over 56 days could be demonstrated. Figure 7 shows the ultrasound images of one of the
test subjects at measurement times D0, D28 and D56. The images were analysed electronically using quantitative determination of dark pixels. The increase in skin density can be seen where there is a decrease in dark pixels. After 28 days, skin density increased by 46.8% and after 56 days by 56.2%.
Profilometry (PRIMOS) As part of the second in vivo study,
D0 D56
Take control of time on the skin In&Out
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AAFF_pmsaInOut_180X125_LPTC_060212.indd 1
Invertage™ is a nutricosmetic solution based on food-grade functional ingredients which are well-know for their beneficial effects in protecting collagen and preventing oxidative stress, fighting aging from the inside out.
anti-aging
showed in vivo a 51% reduction of wrinkle depth after 30 days. This is Lipotec’s In&Out approach for a complete treatment with a greater benefit.
Combining the intake of Invertage™ Capsules with a topical cream containing 5% Trylagen®
March 2012 PERSONAL CARE
06/02/12 11:54
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