ANTI-AGEING
human dermal fibroblasts cultivated with 2% of active ingredient for 24 hours. The gene analysis has been carried on using DNA arrays.
The Undaria pinnatifida extract reactivates genes linked to collagen expression (Fig. 3). The Undaria pinnatifida extract stimulates the expression of proteins involved in collagen maturation such as: Prolyl 4-hydroxylase P4HA1 (+61%) and P4HA2 (+30%): which both have an effect on the early stage of post- translational collagen modification.
Lysyl oxydase LOX (+50%) and Lysyl oxydase-like 2 LOXL2 (+43%): which have an effect on a later stage of collagen synthesis.
In addition it stimulates the expression of: Collagen 1 (alpha chain 1) by +15% (effect illustrated in Figure 4). Collagen 4 (alpha chain 2) by +20%.
Collagen 8 by +37% which is a FACIT (fibril-associated collagens with interrupted triple helices) which binds fibres to their environment.
Lumican (LUM) by +72%, which is a proteoglycan of the SLRP (small leucine-rich proteoglycan) family which binds to collagen fibres to control their organisation (the space between fibres).
Decorin (DCN) by +23%, which is also a proteoglycan of the SLRP (small leucine-rich proteoglycan) family which binds to collagen fibres.
a
80 70 60 50 40 30 20 10 0
P4HA1 P4HA2 LOX LOXL2 COL1 COL4 COL8 LUM
Figure 3: Effect of Matrigenics.14 G on human dermal fibroblast gene expression, linked to collagen synthesis.
The Undaria pinnatifida extract reactivates genes linked to elastic fibre expression (Fig. 5). The Undaria pinnatifida extract stimulates the expression of several genes involved in elastin synthesis and maturation such as: Elastin by +21% (effect illustrated in Figure 6).
Fibrillin 1 by +36%, which participates in elastic fibre maturation.
Lysyl oxydase LOX by +50% and Lysyl oxydase-like 2 LOXL2 by +43%; both of these are involved in elastin maturation.
TIMP2 by +18%, which inhibits b
gelatinase A (or MMP2) activity, which breaks down elastin (elastolysis).
The Undaria pinnatifida extract reactivates genes linked to hyaluronic acid expression. The Undaria pinnatifida extract also stimulates expression of genes involved in the synthesis and the activity of hyaluronic acid such as: Hyaluronan synthase HAS2 (by +68%), this is the enzyme which synthesises hyaluronic acid.
Hyaluronate receptor CD44 (by +31%), which is a receptor for hyaluronic acid which promotes cellular migration.
60 DCN
50
40
Figure 4: Immunolabelling of type 1 collagen fibres produced by human fibroblasts, a) untreated or b) treated with 2% Matrigenics.14 G.
a b
30
20
10
0 ELAST FBN1 LOX LOXL2 TIMP2
Figure 6: Immunolabelling of (tropo)elastin fibres produced by human fibroblasts, a) untreated or b) treated with 2% Matrigenics.14 G.
42 PERSONAL CARE March 2012
Figure 5: Effect of Matrigenics.14 G on human dermal fibroblast gene expression, linked toelastin synthesis and maturation.
Relative expression (% of untreated control)
Relative expression (% of untreated control)
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