SKIN MICROBIOME 47
n Placebo cream n Cream with 1% DEOBIOME NONI PRCF
800 700 600 500 400 300 200 100 0
40 20 0
Initial 3 days + 24h
Figure 6: Clinical evaluation of axillary perspiration rate.
Body odor and the skin microbiome Body odor modulation has historically been a concern for human beings and studying the microbiota involved represents an approach which this topic may naturally be viewed from. The new active Deobiome Noni (INCI:
Morinda Citrifolia Callus Culture Lysate) is an active ingredient made from Morinda citrifolia plant stem cells that introduces the concept of a biological deodorant. By combining an innovative prebiotic technology and the latest advances in plant stem cell technology in skin microbiota treatment, it has been demonstrated how to modulate body malodor while being respectful to the skin microbiota and its ecosystem. The mechanism of action of the active has been evaluated by performing several in vitro and in vivo tests to demonstrate the effective body odor reduction while allowing the natural axillary perspiration.
In vitro efficacy The first strategy of the active (biological activity) is based on its anti-Quorum Sensing (Quorum Quenching) characteristics. A series of in vitro tests were carried out to evaluate the properties of the active that demonstrate the interaction with the microbiota responsible for body odor.
In vitro 1: Analysis of the broad spectrum bacteriostatic and fungistatic effect Firstly, the broad spectrum bacteriostatic and fungistatic effect was demonstrated by quantifying the microbial population in the absence and presence of the active ingredient. It was found that there was significant variation between the untreated cultures and the cultures treated with the active after 24 hours in various species: C. acnes, C. aureus, P. aeruginosa, C. striatum, E. floccosumand M. furfur. The active showed a bacteriostatic effect stopping the growth of the indicated species and maintaining the initial levels but without bactericidal effect (Fig 6).
October 2020
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-100
-34% -43% -51% -52% -63% -67% -100%
Figure 7: Clinical evaluation of odorous volatile molecules reduction. n Placebo cream n Cream with 1% DEOBIOME NONI PRCF
In vitro 2: Anti-Quorum Sensing effect Secondly, the anti-biofilm effect (the formation of biofilm is one of the first consequences of Quorum Sensing activation) in Corynebacterium striatum, Staphylococcus aureus, Cutibacterium acnes and Malassezia furfurwas evaluated by fluorescence staining and Laser Scanning Confocal Microscopy. The active was shown to inhibit the biofilm formation up to 99% while keeping bacterial cells in suspension live (data available on request).
In vivo efficacy In order to demonstrate the efficacy of the active, several in vivo tests were performed.
In vivo 1: Evaluation of armpit odor modulation A first group of 19 volunteers between 20 and 56 years old, applied a placebo cream to one of their armpits and a cream containing 1% of the active to the other armpit to evaluate the armpit odor modulation.
Armpit molecular analysis First, more than 100 molecules responsible for body odor were analyzed by gas chromatography, and demonstrated the reduction of these odorous molecules by 39%. The graph in Figure 7 clearly shows a decrease in these compounds after the application of the active vs placebo.
Armpit odor intensity On the other hand, the average intensity of underarm odor was studied in the 19 volunteers after having applied the active. The study showed a 30% reduction versus initial treatment and down to 82% (data available on request).
In vivo 2: Evaluation of axillary perspiration rate In a second in vivo assay, the rate of axillary perspiration was evaluated in a panel of 8 volunteers, women and men, between 28 and 62 years old, who applied a placebo cream and another cream with a dose of 1% of the active ingredient. The active was shown not to significantly alter natural armpit perspiration.
In vivo 3: Evaluation of feet odor intensity modulation And finally, in a third in vivo test made up of a group of 10 volunteers (23-57-years old), the average intensity of foot odor was evaluated. It was shown that with a cream that contained 1% of the active, a 21% average reduction of foot odor intensity versus initial measurement and down to 47% was possible (data available on request).
Conclusion The active ingredient Kannabia SensePLF
is an
innovative prebiotic treatment that stimulates the skin microbiota to produce a positive in situ postbiotic cocktail that ignites the synthesis of cutaneous oxytocin. This process activates the brain pleasure
centers, induces a better self-perception and positive emotional parameters, what ends up in a healthier and prettier skin. Made from stem cells of the plant
Morinda citrifolia, Deobiome Noni represents a scientific advance for the treatment of body odour, respecting the skin microbiota, thanks to the innovative mechanism of action of the active ingredient, being the first biological deodorant in the market.
-39%
PC PERSONAL CARE NORTH AMERICA
Difference in sweat weight (mg)
% of variation vs untreated
Alcohols Aldehydes
Aromatic alcohols Lactones
Organic acid Oxygen-containing compound
Sulfur-containing compound Terenes
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