6 Additional reported achievements of AEMS technology include:


• Up to 50x faster sample analysis, delivering rich data sets and reducing the risk of missing lead compounds


• Accelerated speed of analysis, capable of up to 3 samples per second – 180 samples/ min, 11,000 samples/hour, 260,000 samples/day


• Low CV’s quantification (5-8%), hence high reproducibility regardless of the matrix


• Sample analysis direct from the plate - No liquid chromatography required, eliminating potential carry over and errors


Sciex Triple Quad™ 5500+ LC-MS/MS System – QTRAP®


Ready


The Sciex Triple Quad™ 5500+ LC-MS/MS System – QTRAP® quadrupole and QTRAP®


from the same sample. The QTRAP® activating a field upgradable license.


Ready, is said to couple triple


functionality in a single system to provide higher data confidence functionality can be implemented at any time by


Combining the two instruments produces an instrument with increased efficiency and dynamic range. Features of the Sciex Triple Quad™ 5500+ LC-MS/MS System – QTRAP®


Shimadzu also announced the release of the Cell Culture Media Analysis Platform, C2MAPTM


-2030, A fully automated workstation for the analysis of cell culture media, the


C2MAP system measures changes of 95 components in a culture supernatant as culturing progresses using LC-MS/MS, resulting in high-quality reproducible data.


Shimadzu also released the Micro-ESI 9030 Flowrate Compatible Ionization Interface compatible and based on the Nexera Mikros Microflow Liquid Chromatography Mass Spectrometry System. Covering the complete range from microflow to semi-microflow, this system allows operators to realise high sensitivity, yet with the reliability and ruggedness of HPLC. The LCMS-9030 Q-TOF system is a research grade mass spectrometer designed to deliver high-resolution, accurate-mass detection with fast data acquisition rates, allowing scientists to identify and quantify more compounds with greater confidence.


Shimadzu also announced a major diversion from the instrument based sales approach of all the major instrument manufacturers in launching the recently developed the Amyloid Mass Spectrometry (MS) Screening Service — a new simple, low-cost blood test for the early screening for Alzheimer’s biomarkers of amyloid-positive subjects. This method enables early and accurate detection of amyloid deposition in the brain with an easy-to-acquire blood sample. Unlike conventional methods, Shimadzu’s new test is minimally invasive, cost-effective and suitable for large-scale deployment.


Ready include:


• Increased Polarity Switching Time: Increased efficiency of positive and negative ion analysis in the same acquisition – with 5 msec in MRM and Scheduled MRM™. Analyse more analytes in a single run with good data quality


• Linear Dynamic Range of up to 6 orders: Produces highly precision, quantified data to see low-level trace detection through to excessively abundant analytes in any application


• QTRAP®


functionality: 12,000 Da/sec enables fast qualitative confirmation of analytes in parallel with MRM quantitative data


TripleTOF® 6600+ LC-MS/MS System - featuring Scanning SWATH®


6600+ LC-MS/MS System is built for flexible large-scale precise quantification. The instrument incorporates simplified low-flow source technology to produce accessible, sensitive and robust quantification; while multiple software improvements increase robustness and maximise system uptime. The key features of the TripleTOF®


OneOmics™ Project in Sciex Cloud The TripleTOF®


6600+ LC-MS/MS System include: • OptiFlow®


Turbo V Source: A single source for all low-flow applications, with flow rates of 100 nL/min – 200 µL/min, the OptiFlow Turbo V Source enables long-term operations to study large sample cohorts


• Up to 100 Hz MS/MS Scan Speeds: Delivers unique qualitative and quantitative capabilities, from fast targeted quantification (MRMHR) to highly multiplexed data- dependent (DDA) and data-independent methods (SWATH)


• Analyst® TF Software 1.8: Scheduled ionisation and target TIC function give the user


temporal control over the number of ions entering the system, eliminating the acquisition of unwanted data and maximising system uptime


Also introduced with the TripleTOF® 6600+, was Scanning SWATH® Acquisition.


Scanning SWATH creates a digital data record of all detectable analytes, capturing more detail about potential markers than its predecessor. Utilising a sliding Q1 window scanned across the mass range, Scanning SWATH produces 4D data where the correlation between fragment and precursor provide better confidence.


Sciex also introduced a new generation of OneOmics™, now integrated into Sciex Cloud. A shared SWATH®


approach by integrating customers data into a cloud-based


environment aimed at efficiently translating big data generated from proteomics, metabolomics, and genomics workflows into meaningful biological results.


Shimadzu Corporation Shimadzu Corporation introduced the following new products at ASMS 2019:


Announced the release of the MALDImini™-1 which is a compact MALDI digital ion trap mass spectrometer. The MALDImini-1 reportedly fits in a space the size of a piece of paper, while allowing fast high-sensitivity measurements and detailed structural and qualitative analyses over a wide mass range, even with sub-microlitre sample volumes.


The small footprint and lower power requirements allow it to be plugged into a conventional AC power supply due to a unique Digital Ion Trap (DIT) technology, with a new design of laser optics, sample stage, and vacuum exhaust system.


The MALDImini-1 can be placed next to the sample preparation area, allowing for a convenient workflow, the sample and matrix are simply spotted on the sample plate, dried, and loaded into the instrument. Taking around five minutes this setup enables users to conduct and repeat even in-depth structural analyses quickly.


The new digital ion trap uses rectangular wave RF to allow ion trapping up to 70,000 Da. This capability, when combined with a MALDI source, enables high-sensitivity MSn analysis in a broad range of applications. The MS/MS and MS3 functionality of the DIT allows researchers to perform comprehensive structural analyses, such as direct glycopeptide analysis, post translational modification analysis, and branched glycan structural analysis. The unit easily integrates with powerful processing tools to analyse data, such as MASCOT for identifying protein digests, SimGlycan™ for processing MSn data for glycans, and eMSTAT Solution™ for statistical analysis.


Figure 3. Shimadzu MALDImini™-1 Acquisition and


It is reportedly the only blood test capable of detecting abnormal amyloid-beta deposition in the brain with 90% accuracy


Thermo Fisher Scientific


Thermo Fisher Scientific headlined with two new high-performance mass spectrometers for protein-based analysis.


The new Orbitrap Exploris 480 mass spectrometer is designed to combine proven technology, advanced capabilities and intelligence-driven data acquisition techniques to enable researchers to deploy MS for more rigorous, high-throughput protein identification, quantitation and structural characterisation. With a smaller footprint than previous generations, the new system maintains high-resolution, mass accuracy and spectral quality, and includes new features to extend uptime and improve serviceability.


Also, the new Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer reportedly provides scientists in academic, government and biopharmaceutical labs with access to a high-performance Orbitrap Tribrid mass spectrometer featuring flexibility and built-in intelligence. The system incorporates advances that improve sensitivity over previous generations and expand the ability to characterise and quantify complex biomolecules and biological systems.


Both new systems can be combined with the Thermo Scientific FAIMS Pro interface, a differential ion-mobility device, offering labs access to a comprehensive solution that improves selectivity and provides increased productivity across a range of proteomics workflows.


Other new introductions included:


The HR Multi-Attribute Method (MAM) is designed to meet the demand for a single, high- resolution mass spectrometry-based workflow to directly assess the quality of increasingly complex biotherapeutics. The workflow replaces the complicated sequence of lower- resolution methods that have previously prolonged characterisation timelines. The HR MAM is supported by the Chromeleon Chromatography Data System (CDS) software.


New analytical software solutions bring together Compound Discover Software, Mass Frontier Software and mzCloud/mzVault libraries to assist customers identify unknown compounds and compare them to known compounds across multiple databases – whether connected to the cloud or offline. The TraceFinder Software 5.0 update, offers quicker and easier access to important information from both the TSQ Quadrupole and Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometry platforms.


Remote instrument access is also possible by using the Thermo Scientific Almanac web- based application to monitor instrument run status and data acquisition in real time through a browser or mobile device.


Figure 4. Thermo Scientific Orbitrap Eclipse Tribrid


INTERNATIONAL LABMATE - JULY 2019


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