45 Proteomics, Genomics & Microarrays High Performance Drosophila ChIP Kits
Porvair Sciences has introduced the first commercial ChIP kit range dedicated to Drosophila (fruit fly). Benefiting from proprietary bead- free ChIP technology, these new kits deliver high-quality ChIP-ready chromatin from as little as 5 Drosophila specimens and from a range of Drosophila tissues.
The fruit fly (Drosophila) is a very widely used model organism for genetic and epigenetic research. However, until now, researchers have had few research protocols at their disposal.
With this new development, Porvair Sciences now offers a single optimised Chromatrap® kit with unique buffers and components for Drosophila chromatin extraction and ChIP. Incorporating correctly orientated capture proteins throughout the inert filter-based ChIP technology, the new Drosophila kits offer outstanding sensitivity which is especially important for detection of binding of low abundant targets. Coupling these advantages with the well documented benefits of bead-free ChIP assay technology provides Drosophila researchers with an easy, sensitive workflow that can be completed in less than 5 hours.
The new Chromatrap range includes Drosophila UniqSeq, ChIP-Seq, Antibodies and Primer Sets. More information online:
ilmt.co/PL/yOgG
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New Monoclonal Antibodies to Mycobacteria
Approximately one third of the world population is infected with Mycobacterium tuberculosis and 10 million new cases occur per year. Diagnosis of active tuberculosis (TB) consists of sputum stain or culture and both methods have poor sensitivities. Rapid and sensitive methods for diagnosis of active TB are sorely needed.
Lipoarabinomannan (LAM) is an abundant and significant surface antigen of this bacterium. It is shed during growth and studies have shown that it can be found in both sputum and urine during active TB disease.
ViroStat has just released two new MabyRab™ Rabbit Monoclonal Antibodies that are specific for Mycobacteria LAM. These new antibodies form a pair for the detection of LAM and are reactive with all strains of Mycobacterium tuberculosis tested. They offer new tools for TB research and potential for rapid detection of this scourge.
These new antibodies join a unique collection of existing Rabbit Monoclonal Antibodies to Candida albicans, Strep A, Strep B, Strep pneumoniae, Aspergillus species and Legionella from ViroStat. Download data sheets for these antibodies at
www.virostat-inc.com.
ViroStat is a primary manufacturer, supplying infectious disease reagents to researchers and rapid test manufacturers since 1985. More information online:
ilmt.co/PL/4Q0Z
49460pr@reply-direct.com
New Stackable Chilling Incubators Ideal for Protein Crystallograpghy
Torrey Pines Scientific announces new EchoTherm™ Stackable/ Programmable Vibration Free Chilling Incubators for protein crystallography and other life science uses.
The incubators are 27 litre capacity and Peltier-based for heating and chilling. They have no compressors or CFCs and are vibration-free making them ideal for doing protein crystallisations. Other applications include incubating marine samples below room temperature, enzyme reactions and deactivations, hybridisations, ligations, storing oocytes, and general lab incubations. Three units may be stacked using two stacker accessories Model IN30-300.
The IN35 is a fully programmable unit that can store 3 programs in memory and features precise temperature ramping both up and down. Temperatures are settable from 4.0ºC to 70.0ºC controllable to ± 0.1°C, and accuracy to
± 0.2ºC. They have RS232 I/O port for remote control and data collection, digital timer in hours, minutes and seconds with user settable Auto-Off, and audible alarms. Chamber size is 12” high by 10” deep by 14” wide and comes with two stainless steel racks with room for four.
The units are shipped with universal power supplies for use anywhere in the world off local line voltages, AC line cord for the country of use, user manual and full 12-month warranty. They are UL, CSA, and CE compliant.
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CRISPR/Cas9 Knockout Cell Lines and Lysates
Amsbio has established a comprehensive portfolio of knock-out cell lines and lysates generated with CRISPR/Cas9 technology. Using CRISPR/Cas9 gene editing technology for the generation of knock-out cell lines and cell lysates ensures the development of useful tools to validate antibody specificity and elucidate gene function. Knock-out cell lines and cell lysates are widely agreed to be the best negative controls to quickly and confidently prove an antibody’s specificity for a target of interest and ensure reproducibility.
Generated in the most widely used HEK293T, HeLa, and A549 cell lines, Amsbio gene edited homozygous knock-out cell lines work in the cells you know and trust. Consequently, you are no longer limited to highly specialised and altered backgrounds. Amsbio offers only 100% knock-out clonal lines meaning you don’t have to work with pools of cells containing only a percentage of knockout cells.
Knock-out cell lysates from Amsbio are made from double knock-out cell lines and provide the perfect negative control for Western Blots to validate the specificity of antibodies or be used in functional assays. Each Amsbio knock-out cell lysate product comes with a cell-line matched wild type control.
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