Proteomics, Genomics & Microarrays
New Pooled Donor Suspension Hepatocytes Enables Reproducibility
Lonza announces the launch of DonorPlex™ Hepatocytes, a new line of high-quality cryopreserved pooled donor suspension hepatocytes, which are the first of their kind to be produced using Lonza’s novel patented manufacturing process. Researchers within DMPK and ADME laboratories can now benefit from this reliable and robust product offering for studying the hepatic metabolism of drugs.
Understanding hepatic drug metabolism is necessary to meet the strict safety-testing requirements for market approval of new products. Researchers typically need to use primary cryopreserved hepatocytes from a large number of donors to obtain a statistically significant result. However, pooled donor hepatocytes currently available on the market commonly suffer from increased lot-to-lot variability, while not being able to meet more specialised application needs.
Lonza’s patented manufacturing process ensures that the DonorPlex™ Hepatocytes exhibit improved phenotype reproducibility. Furthermore, a proprietary algorithm that predicts yield, viability and functional phenotype prior to manufacture ensures individual customer specifications are met.
“Drug discovery scientists require access to high-quality hepatocytes that can deliver consistent and dependable results to expedite new product approval,” said Dr Maureen Bunger, Product Manager for ADME-Tox Solutions at Lonza. “Lonza’s DonorPlex™ Hepatocytes have been designed to do just that. Our patented pooling procedure, in combination with our predictive algorithm, means we can provide our customers with a variety of hepatocyte configurations and sizes that either represent the average population or meet specific metabolism testing and cytochrome P450 (CYP) activity needs.”
The DonorPlex™ Hepatocytes offer a large, comprehensive inventory of cryopreserved single donor suspension hepatocytes, which include a range of 10-, 20- and 50-donor gender-specific and mixed-gender lots.
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Innovative Automated Cell Counter now Awarded US Patent
DeNovix Inc’s CellDrop™, the first imaging cell counter to operate without the need for slides or plastic consumables, has been granted US Patent 10,302,568. DeNovix Inc announces the award of US Patent 10,302,568 for their innovation in the field of cell counting. The CellDrop Automated Cell Counter, engineered with DirectPipette™ Technology, is the first image-based cell counter that completely eliminates the need for slides.
The CellDrop Series features permanent sample surfaces that form a reusable sample chamber. This innovation brings the simple Load, Measure and Wipe Clean functionality of microvolume spectrophotometers to cell counting, making it easier than ever to gain rapid, accurate cell counts and viability measurements. Not only are consumable plastics eliminated, but the instrument also delivers unrivalled levels of performance.
DeNovix Inc has been a developer and manufacturer of life science instruments and assays since 2012. CellDrop cell counters were added to their multi award-winning product portfolio in January 2019 as their newest stand-alone instrument.
“Automated cell counting has always been attractive to researchers, but the cost and environmental impact of plastic slides was a barrier,” stated DeNovix CEO Fred Kielhorn. “Since we introduced the product earlier this year, the market has responded strongly. Scientists immediately recognise that we’ve addressed a key problem with new technology. And we’ve delivered it in a powerful, easy-to-use device.”
The patent recognises DeNovix’s unique, reusable sample surface design. In addition, the patent protects the variable volume counting chamber, which enables measurements across the widest range of cell densities - 7x10e2 to 2.5x10e7 cells/mL. Samples are easily removed with a dry laboratory wipe after analysis.
The CellDrop Series is a line of image-based, automated cell counters. Systems include a high definition 7 inch touchscreen for live preview and instant review of results. Pre-installed EasyApps® software includes applications for AO/PI, Brightfield, Trypan Blue and GFP and user customized methods. The software delivers rapid cell counts, viability reports and transfection efficiency assays. All instruments feature Wi-Fi, USB and Ethernet connectivity, allowing users to easily export data via email, network drives, network printers or USB drive. CellDrop cell counters are available in dual fluorescence and brightfield or brightfield only models.
More information online:
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Highly Effective Protein Clean-Up Microplate
The Protein Crash technique used by the Porvair Sciences P3 microplate is highly effective at removing proteins from biological samples prior to analysis.
The P3 microplate works by denaturing the protein upon addition of solvents such as acetonitrile or methanol. The proteins ‘crash’ or precipitate out of solution to forms large sticky globules that are then trapped on an open-pore structure filter. Underneath this filter is a highly hydrophobic filter which retains all liquids above it. When a vacuum is applied to the P3 microplate, the liquid containing the analytes of interest is efficiently drawn through the filter and into a collection plate below leaving behind unwanted proteins that can interfere with downstream applications.
The novel dual filter technology in the P3 microplate not only retains unwanted proteins and has good flow rates, it ensure no leakage occurs prior to collection by vacuum. Designed and precision manufactured to conform with ANSI /SLAS guidelines, the automation- friendly P3 microplate enables 96 samples to be filtered simultaneously, accelerating your biological sample clean-up prior to analysis.
The P3 microplate is manufactured from specially selected polypropylene to ensure very low extractables. The dual filters in the P3 plate are produced from an inert material to ensure no adsorption of components from your biological samples thereby eliminating the chance of cross contamination or loss of sample integrity.
To download a copy of the report ‘Biological Sample Preparation using Solid Phase Extraction and Protein Crash techniques in 96 well plates’ please visit
https://bit.ly/2GoOnxK. More information online:
ilmt.co/PL/BOWm
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