5
Further software developments by Dr Yates continue to empower molecular and cellular biology research, including peptide and protein quantitation, identifi cation of posttranslational modifi cations, and the use of DNA sequences to enable proteogenomic methods.
Al Yergey MS Scientist Award
Dr Jeffrey Shabanowitz, a Principal Scientist in the Hunt Laboratory at the University of Virginia, was the recipient of the new Al Yergey MS Scientist Award. The Al Yergey Mass Spectrometry Scientist Award is sponsored by ASMS to recognise dedication and signifi cant contributions to mass spectrometry-based science by ‘unsung heroes’. This award is named in memory of Al Yergey a well-respected scientist who was known as a dedicated mentor.
Dr Shabanowitz has worked with Professor Donald Hunt at the University of Virginia for forty years and has coauthored more than 330 peer-reviewed scientifi c papers and is the co-inventor on ten patents. The methods and instrumentation he helped to develop played a major role in the development of peptide sequence analysis by tandem mass spectrometry which now underpin the fi eld of proteomics, and have led to major breakthroughs, especially in immunology and epigenetics research.
Biemann Medal
The Biemann Medal is awarded to an individual early in his or her career to recognise signifi cant achievement in basic or applied mass spectrometry. Dr Sarah Trimpin, Professor of Chemistry at Wayne State University was awarded the 2019 Biemann Medal for her discovery and development of novel ionisation processes. Dr Trimpin’s observation of highly charged protein ions in an atmospheric pressure MALDI experiment led to her discovery that ionisation can occur by passing compounds through the mass spectrometer inlet. She demonstrated that this approach provides sensitivity often better than electrospray ionisation or MALDI.
Through her studies, Dr Trimpin discovered solid matrices that spontaneously produce multiple charged ions when exposed to vacuum (termed matrix-assisted ionisation, MAI) needing no heat, nebulising gases, laser, or voltage with a very low chemical background achieved for a variety of compounds, including proteins as large as bovine serum albumin (66 kDa).
Ron A. Hites Award Outstanding Research Publication in JASMS
The Ron Hites Award, which includes $2,000 and a certifi cate, is awarded to a scientist for an original outstanding research publication, based on its novelty, technical and presentation quality, potential stimulation of future research and the impact on other future applications.
The 2019 Award was presented to Dr Julia Laskin from Purdue University and her co- authors for their paper entitled ‘Towards High-Resolution Tissue Imaging Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Coupled to Shear Force Microscopy’; Son N. Nguyen, Ryan L. Sontag, James P. Carson, Richard A. Corley, Charles Ansong, and Julia Laskin; J. Am. Soc. Mass Spectrom. (2018) 29:316Y322.
New Product Roundup
In the interests of impartiality companies introducing new instruments and products are again listed in alphabetical order, we have attempted to feature the majority of new product introductions for 2019.
Agilent Technologies
The new Agilent Infi nityLab LC/MSD iQ System, released at ASMS, incorporates ‘designed-in’ smart features, software, and hardware. The Agilent Infi nityLab Liquid Chromatography/Mass Selective Detector iQ (LC/MSD iQ) system provides mass detection for quick confi rmation of compounds providing more specifi city and selectivity than a UV detector - even for chromatographically unresolved compounds.
The Infi nityLab LC/MSD iQ system is designed to sit beneath the Agilent’s Infi nityLab HPLC instruments stack introduced in 2018, saving lab space. The system is designed to be serviced without dismantling the stack and the new Infi nityLab Flex Bench MS enables mobility with easy access to all the modular system components.
New software features include an Auto Acquire mode for method setup and routine operation utilises the OpenLab CDS Software. A new release of Agilent’s MassHunter WalkUp Software for open-access drug discovery and chemistry labs has a touch-screen enabled interface and preconfi gured analyses and reports.
Other new Agilent products, include the 6546 LC/Q-TOF System provides the acquisition of high-resolution data across a wide dynamic range and includes Q-RAI (Quadrupole-Resolved All Ions), a data-independent acquisition mode that reduces the complexity of MS/MS spectra. An updated MassHunter Software Suite was launched with the 6546 system.
Figure 1. Infi nityLab LC/MSD iQ.
Bruker The main introduction of ASMS in 2019 for Bruker was the new timsTOF fl eXTM
spectrometer which includes a software-switchable MALDI source adapted to the ESI timsTOF ProTM
platform.
The new, combined ESI/MALDI capability is reported to enable spatially-resolved omics, SpatialOMxTM timsTOF fl eX has a 10kHz SmartBeamTM
for rapid, label-free MALDI imaging at high-spatial resolution, preserving the 4D proteomics and phenomics sensitivity of the timsTOF Pro in ESI mode.
With the SpatialOMx approach, researchers can see spatial molecular distributions in tissues from MALDI imaging, to guide 4D omics molecular expression studies, e.g. on proteins, low-level cancer antigen peptides, lipids, glycans, metabolites, or xenobiotics, which cannot be observed by traditional staining or labelling techniques. MALDI-guided SpatialOMx permits the specifi c targeting of cell sub-populations for subsequent ESI-TIMS/PASEF-based data dependent acquisition (DDA) or data independent acquisition (DIA) 4D proteomics or 4D lipidomics/ metabolomics. Both can now be performed on a single instrument.
Figure 2. timsTOF fl eXTM Bruker also launched IntelliSlidesTM specifi cally designed to automate timsTOF fl eX
workfl ows. The IntelliSlides come pre-inscribed with software-readable ‘teach marks’ on the conductive surface to indicate where to place the tissue sample, a bar code and tracking number. With specialised software, IntelliSlides now make MALDI Imaging even easier. The SCiLS Lab 2020 MALDI imaging software, now integrated with MetaboScape5.0 for automated annotations of lipids and metabolites in tissue molecular images in spatialOMx.
4D Phenomics and 4D Proteomics Innovations
Bruker’s timsTOF™ Pro launched in 2018 for nLC-TIMS-MS/MS 4D proteomics has been further enhanced by combining PASEF with DIA, in diaPASEF for high sensitivity bottom- up proteomics. While the duty-cycle of DDA PASEF dramatically improved sensitivity and depth of coverage using short nanoLC runtimes, the stochastic nature of DDA results in missing values, an issue signifi cantly improved by diaPASEF workfl ows, or by match- between-runs (MBR) for ddaPASEF.
The diaPASEF workfl ow, shown at ASMS 2019 as a work-in-progress workfl ow available to methods development customers, uses overlapping windows in the ion mobility spectrum to trigger MS/MS, effi ciently using the quadrupole to transmit the precursor ions at high sensitivity. The new diaPASEF workfl ow typically results in a 30% improvement, now with over 7,000 proteins identifi ed in a 120 minute single-shot experiments with 200 ng of HeLa digest injected. Data analysis, including 4D feature alignment in mass, retention time, ion mobility and intensity, is performed using the new Mobi-DIK software, which is based on the OpenMS software.
New Consumables and Software for 4D Proteomics
Bruker and PreOmics GmbH announced a co-development and co-marketing agreement for PreOmics iST sample preparation technology on the timsTOF Pro. The inStageTip (iST) technology removes detergents, polymers, salts, lipids and other contaminants. New iST protocols enable sample preparation with a signifi cant time advantage (over 40 hrs saved) compared to common protocols.
For proteomics software, Bruker and Genedata announced a partnership for the Genedata Expressionist software platform, which supports the timsTOF Pro 4D omics format.
New Ultra-high Sensitivity 4D Lipidomics Workfl ow
Bruker announced advances to its ultra-high sensitivity 4D lipidomics workfl ows on the timsTOF Pro and timsTOF fl eX platforms, showing high-sensitivity measurements using LC-TIMS-MS/MS. Optimisation of the PASEF 4D lipidomics methods now enables double the number of identifi ed lipids in a single-shot analysis. This workfl ow employs nanoLC- TIMS-PASEF to quantify approximately 500 lipids with high quantitative accuracy and reproducibility from just a few thousand cells, in addition to building a library of more than 1,000 accurate CCS values from human plasma, mouse liver and human cancer cells.
Sciex Sciex released the following new products and technologies at ASMS.
Acoustic Ejection Mass Spectrometry (AEMS) technology, incorporating the Open Port Interface and Acoustic Droplet Ejection, is introduced before commercialisation and is stated to be Echo MS upon commercialisation.
In early testing, AEMS technology has reportedly shown the potential to reduce screening times, from 115 days to 4 days for 1 million compounds due to reductions in analysis time.
, on a single instrument. The 3D laser with pixel fi delity
mass
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