41 Proteomics, Genomics & Microarrays New Tissue Clearing Kits and Reagents for Faster, Accurate 3D Imaging
Abcam’s tissue clearing kits and reagents enable easy and rapid clearing for 3D imaging of tissues and cell culture models. Traditionally, 3D imaging of thick tissue sections and 3D cell culture models (organoids, microtissues, spheroids) has presented many technical challenges, including laborious and time-consuming tissue processing, layer-by-layer 2D imaging of thin sections and in silico 3D reconstruction. By contrast, tissue clearing reagents can expedite detailed 3D imaging by reducing the need for these steps, while providing a truer reflection of biological structures.
Developed with Visikol’s HISTO™ technology, Abcam’s tissue clearing kits use simple protocols, standard laboratory equipment, and are compatible with immunostaining, fluorescent protein and chemical dyes. Clearing is also reversible, for conventional H&E or IHC staining following 3D imaging.
To facilitate the set-up and use of Abcam’s tissue clearing kits, a selection of recombinant antibodies for key markers (Neun, S100 and Iba1) have been validated for use with the kits. Tissue clearing reagents can be purchased as part of a kit or as individual products, for greater flexibility.
The image shows Albumin antibody ab207327 (yellow), CD68 antibody (green), Vimentin antibody (red), and DAPI (blue) used with 3D Cell Culture Clearing Kit ab243299 to clear and stain a 3D hepatocyte cell culture spheroid.
More information online:
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First In vitro Diagnostic IHC Test to Detect ROS1 Protein in Cancers Launched
Roche have announced the global launch of the VENTANA ROS1 (SP384) Rabbit Monoclonal Primary Antibody, the first and only in vitro diagnostic ROS1 immunohistochemistry (IHC) assay. The test detects the presence of ROS1 protein in tissue, and may be useful in identifying ROS1-positive cancer cases. Guidelines from the College of American Pathologists and the National Comprehensive Cancer Network recommend ROS1 testing for confirmed lung adenocarcinoma cases.
As these types of cancer are rare, found in up to 2% of non-small cell lung cancer cases, the use of a ROS1 IHC biomarker may provide a cost-effective and efficient means of identifying cases with elevated ROS1 protein expression before confirming by another method, such as by fluorescence in situ hybridisation (FISH) or next-generation sequencing.
“Our highly sensitive ROS1 test is the first in vitro diagnostic IHC available for recommended lung cancer testing guidelines, with the added benefit of rapid turnaround time,” said Jill German, Head of Roche Tissue Diagnostics. “While this is important in non-small cell lung cancer cases today, ROS1 is also being investigated in a number of clinical trials in other cancer types.”
The VENTANA ROS1 (SP384) Rabbit Monoclonal Primary Antibody is a US class I/CE in vitro diagnostic device and is available for use on Roche’s BenchMark series of automated staining instruments.
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