Terms & Definitions • 269 peak area


The area of the peak on a chromatogram produced by a substance subjected to chromatographic analysis. Proportional to the concentration of the substance.


• 270 peak area ratio


Ratio of the instrument response peak area of the target compound in the sample to the peak area of an internal standard.


• 271 peak capacity


Chromatographic selectivity demonstrated by showing the maximal number of peaks that can be baseline resolved in a given chromatographic time window.


• 272 peak fronting


Distortion of the classical Gaussian form of a chromatographic peak due to one or more factors, including sorption isotherms and column overload. The analyte molecules at the center of a migrating band move slower than those on the outer edges.


• 273 peak height


The instrument response height of the peak on a chromatogram produced by a substance subjected to chromatographic analysis. Proportional to the concentration of the substance.


• 274 peak height ratio


Ratio of the instrument response peak height of the target compound in the sample to the peak height of an internal standard.


• 275 peak tailing


Distortion of the form of a chromatographic peak, which deviates from its theoretical Gaussian form due to one or more factors, including secondary interactions of the analyte with separation materials.


future science group www.future-science.com S63


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