66
Using natural oil to improve skin barrier function
Mike Martinez, CEO, and Aaron Reber, Account Executive, Natural Plant Products, Inc, assess Daikon Radish Oil’s ability to improve skin barrier function
Daikon Radish Oil (DRO) is a fully-refined triglyceride derived from the seeds of Raphanussativus grown in the Willamette Valley of Oregon. DRO imparts a delicate skin feel and emolliency, and its aesthetics differ qualitatively from other vegetable oils.
Objective To evaluate the ability of DRO to improve skin barrier function in comparison to three well-known emollients used in skin care. • Number of subjects: Twenty-seven • Test Area: Volar forearm • Test Products: Daikon Radish Oil, Meadowfoam Seed Oil, 100 cps dimethicone, and isopropyl palmitate (IPP).
• Endpoint: Trans-Epidermal Water Loss (TEWL) – A reduction in trans-epidermal water loss indicates measured increase in skin barrier function.
• Instrument: DermaLabEvaporimeter (Cortex Technology, Hadsund, Denmark)
Methodology After a three day washout on forearms with a neutral soap bar and no usage of personal care products on the test sites, subjects reported to the testing facility. The testing facility was maintained at 20-22ºC and 30-50% relative humidity. Four test sites measuring 4x4cm were demarcated on the volar forearms at least 2cm from the wrist and elbow, with at least 2cm between adjacent test sites. After test site demarcation, subjects remained in the testing facility with the test sites uncovered and
Products were assigned to test sites using a randomised design with each subject having all four products applied. Subjects remained in the test area with the test sites uncovered and exposed for 30 minutes at which point the first TEWL measurement was taken. Participants were sequestered in the testing facility for the balance of the study, and TEWL measurements were taken at 1 hour, 2 hours, and 4 hours post-application after allowing participants to equilibrate for 15 minutes in the measurement room.
Statistical Analysis Mean reduction in TEWL from baseline was calculated for each treatment. The data were subjected to a two-tailed paired t-test with significance set at a p-value of 0.05. Treatments were
exposed for 15 minutes. After this equilibration period, a baseline measurement was taken at each test site. Trained clinical staff then applied 1mg/cm2 of a test product to the test sites using a finger cot.
compared to the untreated baseline and against other treatments.
TEWL (Barrier Function) Results DRO, MSO, and 100 cps dimethicone significantly reduced TEWL at 30 minutes, 1 hour, 2 hours, and 4 hours post-application compared to the baseline. IPP significantly reduced TEWL at 1 hour and 2 hours post-application. The performance of DRO was significantly different from the performance of IPP at the 30 minute, 1 hour and 2 hour marks.
Conclusions DRO decreased TEWL and significantly increased barrier function compared to isopropyl palmitate at all measurement points during the study demonstrating the enhanced utility of DRO that can be achieved, while maintaining the light skin feel associated with IPP. There was no statistical difference in performance between DRO, MSO, and 100 cps dimethicone demonstrating that DRO performs comparably to well-known cosmetic emollients. This data is presented in good faith and is based on information believed to be reliable, which is offered solely for evaluation, investigation, and verification of the numerous factors that may affect results. Natural Plant Products, Inc. produces and sells Daikon Radish Oil with the understanding that purchasers will perform their own tests to determine the suitability of this product for their own particular use.
Natural Plant Products, Inc, Stand F15
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120 |
Page 121 |
Page 122 |
Page 123 |
Page 124 |
Page 125 |
Page 126 |
Page 127 |
Page 128 |
Page 129 |
Page 130 |
Page 131 |
Page 132 |
Page 133 |
Page 134 |
Page 135 |
Page 136 |
Page 137 |
Page 138 |
Page 139 |
Page 140 |
Page 141 |
Page 142 |
Page 143 |
Page 144 |
Page 145 |
Page 146 |
Page 147 |
Page 148 |
Page 149 |
Page 150 |
Page 151 |
Page 152 |
Page 153 |
Page 154 |
Page 155 |
Page 156 |
Page 157 |
Page 158 |
Page 159 |
Page 160 |
Page 161 |
Page 162 |
Page 163 |
Page 164 |
Page 165 |
Page 166 |
Page 167 |
Page 168 |
Page 169 |
Page 170 |
Page 171 |
Page 172 |
Page 173 |
Page 174 |
Page 175 |
Page 176 |
Page 177 |
Page 178 |
Page 179 |
Page 180 |
Page 181 |
Page 182 |
Page 183 |
Page 184 |
Page 185 |
Page 186 |
Page 187 |
Page 188 |
Page 189 |
Page 190 |
Page 191 |
Page 192 |
Page 193 |
Page 194 |
Page 195 |
Page 196 |
Page 197