25
smoothness was improved. (Figure 2a, day 56). Anti-Wrinkle effect of PhytoCellTec™
Argan
A clinical study was carried out over 56 days with 21 women aged from 39 to 61 having sun damaged skin (Phototype I-III). A cosmetic product containing 0.4% PhytoCellTec™
Argan was applied twice per day
on the crow’s feet area (wrinkle depth) by performing the vehicle-controlled half side comparison. Wrinkle depth was determined by PRIMOS pico at days 28 and 56. Results showed that the active successfully reduced the wrinkle depth in crow’s feet area by 19% after 28 days of treatment and by 26 % after 56 days of treatment (Figure 3).
PhytoCellTec™ The capacity of PhytoCellTec™
Argan recovers Skin Density Argan to improve
dermal tissue density was evaluated by visualising the structure of the dermis using ultrasonography. The ultrasonic wave generates echoes when it is partially reflected at the boundaries between different tissue structures. In the ultrasonographic images the
D28 D56
30% ______________________________ 25% ______________________________ 20% ______________________________ 15% ______________________________ 10% ______________________________ 5% ______________________________ 0% ______________________________
** *
*p=0.0005 versus initial conditions **p=0.001 versus initial conditions
Figure 3. Wrinkle depth after 28 days and 56 days of treatment with PhytoCellTec™ placebo and to initial conditions
Argan relative to the
collagen and elastic fibres structure of an intact dermis yields many reflections visible as bright colours. However, disruption of this regular architecture leads to weaker reflections and dark patches known as subepidermal low-echogenic bands (SLEB). These signals are commonly found in aged and photo-damaged skin. Changes in SLEB of 21 volunteers with an average age of 49 were monitored by analysing ultrasonographic images taken from their forearms before and after treatment, with either a 0.4 % PhytoCellTec™
Argan cream or a
corresponding placebo (Figure 4a). Results showed that the treatment with PhytoCellTec™
References Argan leaded to a clear increase in
skin density of 13% compared to the placebo and after 8 weeks treatment (Figure 4b).
Conclusions Composed of fibroblast cells embedded in a collagen-elastin matrix, the dermis confers firmness to the skin. Exhaustion of dermal fibroblasts is the very early onset of wrinkle formation and loss of
Epidermis Dermis SLEB
Epidermis Dermis
Biernaskie J., Paris M., Morozova O., Fagan B.M., Marra M., Pevny L., Miller F.D. SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells. Cell Stem Cell. 2009; 5 (6), 610-623. Driskell R. R, Clavel C., Rendl M.,Watt F.M. Hair follicle dermal papilla cells at a glance. Journal of Cell Science 2011; 124, 1179-1182. Schmid D., Zuelli F., Belser E. Vitalisation of dermal stem cells for skin rejuvenation. Personal Care. 2011; July, 33-35.
D28 D56
14% ________________________________ 12% ________________________________ 10% ________________________________ 8% ________________________________ 6% ________________________________ 4% ________________________________ 2% ________________________________ 0% ________________________________
* D0 D56
Figure 4a. Density of the Upper Dermis determined by ultrasonography
*p=0.025 versus initial conditions **p=0.004 versus initial conditions
Figure 4b. Increase of skin density compared to the placebo and to initial conditions
** +12.7%
firmness. Dermal stem cells are essential for a lifelong regeneration and repair of this delicate matrix. PhytoCellTec™
Argan is the very first active
ingredient able to protect and vitalise human dermal precursor cells at the same time. Clinical results showed a tightening of the dermal connective tissue compared to initial conditions, leading to a much smoother dermis-hypodermis junction. PhytoCellTec™ Argan helped the skin to regain its firmness and significantly reduced wrinkle depth in crow’s feet area.
➞ ➞
➞ ➞
Decrease in wrinkle depth relative to placebo and D0 (%)
Increase in skin density relative to placebo and D0 (%)
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