42 SKIN CARE


induced a significant 48% inhibition of lipase secretion; this result allowed us to validate the experiment. In our study we observed that the P. lobata


extract significantly decreased lipase release by S. aureus at both concentrations tested. We obtained a significant decrease of 65% and 84% respectively with 0.5 and 1% of the plant extract. This activity was obtained without any significant decrease in bacteria population density measured by optical density recorded at 600nm (data not shown). These results showed the capacity of P. lobata extract to protect skin from S. aureus assaults namely skin colonization and barrier disruption by lipase activity.


Conclusion Regulating bacterial QS signaling by utilizing QS interfering molecules or products is a promising strategy to control the bacterial harmfulness expressed through virulence factors production. The gram-positive S. aureus and gram-negative P. aeruginosa on which we have focused are known to be involved in skin conditions where there are skin barrier disruption and inflammation. For both bacteria, harmfulness is under


control of QS. We have shown that QS inhibition of these bacteria in vitro can be achieved using natural plant extracts: Litchi sinensis extract exerted a significant inhibition of P. aeruginosa QS followed by a decrease of the bacteria swarming involved in bacteria colonization. Pueraria lobata effectively inhibited


secretion of QS molecule AIP by S. aureus and subsequently decreased secretion of lipase involved in skin colonization and skin barrier impairment, which is fundamental for skin beauty, wellbeing and health. These natural extracts have been


characterized by their flavonoid’s component, emphasizing again the potential and the interest of these family of plant metabolites for cosmetic application. Finally, targeting this mechanism of QS


inhibition provides some perspectives in the development of cosmetic active ingredients delivering skin benefits. We can control harmful bacteria on skin without disturbing the subtle balance of skin microbiota, which lives in symbiosis with skin cells. We can also reduce the appearance of


bacterial resistance as often observed with products having antibacterial properties. The properties evidenced in vitro by these two botanical extracts are expected to be beneficial for dry, sensitive or reactive skin which are prone to development of inflammation. Overall skin wellbeing will be preserved and increased in the face of bacterial and environmental assaults.


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5. Hong S-W, Choi E-B, Min T-K, Kim J-H, Kim M-H, Jeon SG, Lee B-J, Gho YS, Jee Y-K, Pyun B-Y, Kim Y-K. An Important Role of a-Hemolysin in Extracellular Vesicles on the Development of Atopic Dermatitis Induced by Staphylococcus aureus. PLOS One. 2014


6. Hentzer M, Wu H, Andersen JB, Riedel K, Rasmussen TB, Bagge N, Kumar N, Schembri MA, Song Z, Kristoffersen P, Manefield M, Costerton JW, Molin S, Eberl L, Steinberg P, Kjelleberg S, Høiby N and Givskov M, Attenuation of Pseudomonas aeruginosa virulence by Quorum Sensing inhibitors. The EMBO Journal. 22(15), (2003), Aug, 3803- 3815


PC


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PERSONAL CARE February 2024


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