14
Fast Screening for Vitamins and Dyes with the ACQUITY UPLC H-Class System Goal
To successfully analyse a series of water-soluble vitamins and food dyes in health beverages using the ACQUITY UPLC® consumption.
H-Class System, with minimum sample preparation, a short run time, and minimum solvent
Background Vitamin fortification of beverages is an increasing trend that has become popular due to the poor level of nutrition that can occur in today’s fast-paced lifestyle. Manufacturers add vitamins to many beverages and also use dyes to aesthetically enhance the appearance of these products. Beverage producers are legally required to accurately label their products with vitamins, dyes, and other additives that are used in the formulation of these commodities. In the case of food dyes, stringent requirements exist as to what can be added to beverages intended for human consumption. Dyes can vary from country to country. In the United States, the three main colorants are yellow 5, red 40, and blue 1.
These compounds are currently analysed individually or in small groups using a variety of methodologies such as microbiological assays, colorimetric analysis, titrimetric procedures, fluorimetry, and HPLC. Typical HPLC methods for the analysis of a selection of food dyes can run up to 30 minutes, or, for the analysis of vitamin B6 alone, 18 minutes. The ability to simultaneously analyse water-soluble vitamins and food dyes using a single, fast, and straightforward UPLC®
solution provides businesses with the potential to increase sample turnover
and reduce costs associated with labour and training. This improvement in efficiency can subsequently result in higher productivity and increased revenue.
The solution
The separation of 11 water-soluble vitamins and three food dyes was achieved using the ACQUITY UPLC H-Class System in less than 8 minutes. Analytes were injected onto an ACQUITY UPLC HSS T3 Column and eluted with a water/methanol (0.1% formic acid) gradient. PDA detection was employed at the optimum wavelength for each analyte.
B-vitamin-fortified water was procured commercially. Following filtration through a 0.45 micron membrane, the sample was injected onto the column. No additional sample preparation was required. The label claim of vitamins B3, B5, and B6 was confirmed by UPLC analysis. Several unknown analytes were also detected.
Summary
Featuring multi-solvent blending capabilities and a flow-through-needle design, the ACQUITY UPLC H-Class System provides the familiarity of HPLC instrument operation along with the improved chromatographic performance and throughput of UPLC. The ACQUITY UPLC H-Class System with PDA detection here provides a rapid and robust method for the analysis of water-soluble vitamins and food dyes in beverages that claim such health benefits. The system allows for the analysis of multiple analytes in a single platform using a straightforward workflow that is a reliable alternative to lengthy HPLC separations.
2024ad@reply-direct.com Simplified Method Development in HPLC
Shimadzu presented its Nexera Method Scouting system at the Live Lab Food Analysis exhibit during analytica 2014. This system solution tests up to six columns and eight mobile phases while simplifying method development in high-performance liquid chromatography. Method Scouting provides important and reliable data for determination of analysis conditions. The Method Scouting Solution software simplifies batch creation and ensures a fast and reliable route to analysis start-up. The Nexera Method Scouting system is based on the Nexera UHPLC, an all-round LC system for ultra-high-speed analysis. It enables automated switching between up to 96 analysis conditions, thereby increasing productivity. The system enables continuous data acquisition overnight and contributes to an efficient method development process. The analysis schedule is generated automatically in the Method Scouting Solution software. The LabSolutions (Shimadzu’s HPLC software) browser function supports users in finding a suitable method.
The Nexera Method Scouting system reduces analysis preparation time and human error, which can occur when manually setting the method and analysis schedule. Mobile phases and columns can be selected from libraries. Additional scouting conditions required, such as gradient slope, can be specified in the Method Scouting Solution software. Column rinsing and equilibration can also be adjusted in the software. Colour-coding of the column and solvent tubings
corresponds with the colour-coding in the software’s graphical user interface, further increasing the ease of use.
The Method Scouting system can be connected to a mass spectrometer, which further supports peak assignment. Peaks can then be identified without the need for reanalysis of individual sample components. In combination with the Drylab chromatography modelling software for further method optimisation and the Validat toolbox for method validation, the Nexera Method Scouting software provides a good basis for efficient HPLC method development and implementation.
29590pr@reply-direct.com New OI Analytical GC Products Distributor in the UK & Ireland
Davidson Analytical Services Ltd, based in Nottingham, UK, has now formalised it’s agreement with OI Analytical, College Station, Texas, USA to become the exclusive UK & Ireland distributor for the OI Analytical Gas Chromatography product range.
Andrew Davidson, director of Davidson Analytical, commented: “Our new partnership with OI Analytical is an exciting new opportunity for us. OI have an interesting and innovative range of GC products that compliments our existing range of products and services perfectly. We are looking forward to bringing our values of personal service and commitment, to supporting existing OI customers and working to introduce new users to OI’s great products.”
The product range includes selective GC detectors, such as, the PFPD (highly sensitive and selective sulphur detection), XSD (halogen specific, no radioactive source), PID (alkenes and aromatics), patented tandem detectors and the Eclipse 4660 Purge & Trap system.
26057pr@reply-direct.com
Analysis of commercially-available B-fortified water, confirming the presence of vitamins B3, B5, and B6.
Separation of 11 water-soluble vitamins and 3 food dyes by UPLC using the ACQUITY UPLC H-Class System.
INTERNATIONAL LABMATE - APRIL 2014
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84
