Terms & Definitions • 315 polymerase chain reaction (PCR)


A molecular biological method used to enzymatically amplify a single or few copies of a particular DNA sequence using short DNA sequences called DNA primers.


Sponsored by • 316 population-specific cut point


A threshold level determined for and applied to a target population to define the presence or absence of a response.


• 317 post-column infusion


Technique for the evaluation of matrix effect profiles in LC–MS analysis, by injection of a solvent blank or matrix blank into the chromatography column while simultaneously infusing analyte by a syringe pump to the column effluent before the detection device.


• 318 post-translational modification (PTM)


Chemical modification to poly peptides, such as the attachment of functional groups (phosphate, carbohydrates), during their biosynthesis, but after their translation from the mRNA sequence.


• 319 PrD-LC–MS


A bioanalytical method where matrix containing the analyte protein is directly digested using trypsin or another specific protease followed by quantitative analysis of the surrogate peptide by LC–MS/MS; “reagent free” methods use no immuno- or affinity enrichment of either protein analyte or surrogate peptide. In specific cases mass spectrometer methods other that MS/MS may be applied.


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