The Bioanalysis Glossary • 297 peak capacity
Chromatographic resolution demonstrated by showing the maximal number of peaks that can be baseline resolved in a given chromatographic time window.
• 298 peak fronting
Distortion of the classical Gaussian form of a chromatographic peak due to one or more factors, including sorption isotherms and column overload. The analyte molecules at the center of a migrating band move slower than those on the outer edges.
• 299 peak height
The instrument response height of the peak on a chromatogram produced by a substance subjected to chromatographic analysis. Proportional to either the concentration or mass of the substance, depending on the mode of detection.
• 300 peak height ratio
Ratio of the instrument response peak height of the target compound in the sample to the peak height of an internal standard.
• 301 peak tailing
Distortion of the form of a chromatographic peak, which deviates from its theoretical Gaussian form due to one or more factors, including secondary interactions of the analyte with separation materials.
• 302 peak tailing factor
Also known as ‘symmetry factor’; a value that reflects the (a)symmetry of a chromatographic peak.
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Bioanalysis (2018) 10(21) Suppl. 1
future science group
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