92 Using Fluorescence Imaging for Improved Outcomes in Skin Cancer Surgery
University of Rochester Professor Michael Giacomelli recently received NIH funding for his project, ‘Fluorescence microscopy for evaluation of Mohs surgical margins’. His research objective is to enable real-time evaluation of pathology in skin tissue with an order of magnitude reduction in processing time as compared to frozen sections.
The most common forms of cancer worldwide are basal cell carcinoma and squamous cell carcinoma. Mohs surgery is a widely used technique for the treatment of nonmelanoma skin cancer that obtains extremely low recurrence rates by imaging tissue as it is removed from the body to ensure complete resection. Mohs Micrographic Surgery, also called Mohs Surgery, is a specialised technique to remove non-melanoma skin cancers.
Nonmelanoma skin cancer (NMSC) is primarily diagnosed by histologic analysis of skin biopsy specimens in kerosene sections, which takes days to weeks before a formal diagnosis is made. Two-photon fl uorescence microscopy (TPFM) has the potential for point-of-care diagnosis of NMSC and other dermatologic conditions, which could allow diagnosis and treatment at the same site.
Professor Giacomelli developed a way to image multiple depth slices without frozen section processing‚ by implementing two-photon imaging, widely used in the neurosciences to noninvasively image slices at different depths in living brains. The lab team’s research has adapted these fl uorescence imaging technologies with rapid tissue labelling and image processing technologies. This enables real-time assessment of pathology in skin tissue. processing time compared to frozen sections has been reduced by an order of magnitude. The data collection time is further reduced using the lower-noised silicon photomultiplier detectors developed by Giacomelli’s group.
Toptica Photonics’ femtosecond laser system - FemtoFiber ultra 920 - chosen for the next iteration of the Giacomelli lab’s microscope is a possible solution for applications in non-linear microscopy like two-photon excitation of fl uorescent proteins and SHG based contrast mechanisms. With an emission wavelength of 920 nm, it provides the highest peak power for imaging with green and yellow fl uorescent protein markers (GFP, YFP) commonly used in pathology, neurosciences and other laser-related biophotonic disciplines. Researchers appreciate the usability of the system since it is both, maintenance free and very compact.
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New Cryo-TEM to Help Accelerate Drug Discovery Research
The Thermo Scientifi c Glacios 2 Cryo-Transmission Electron Microscope (Cryo-TEM) is a powerful microscope with new automation and high-resolution imaging capabilities designed to help cryo-electron microscopy (cryo-EM) researchers of varying experience levels accelerate structure-based drug discovery. This advanced, fast and cost-effi cient method for drug design may enable customers to accelerate the pace of research for debilitating disorders like Alzheimer’s, Parkinson’s, and Huntington’s diseases, as well as research for cancer and gene mutations.
The Glacios 2 Cryo-TEM includes automation features designed to extend accessibility to a range of cryo-EM techniques, including single particle analysis, cryo-electron tomography (cryo-ET) and microcrystal electron diffraction (MicroED). Additional highlights include fringe-free imaging for the acquisition of more usable images per foil hole, increased throughput compared to other commercially available optical alignment solutions and a new enclosure and hardware improvements built to offer enhanced performance compared to prior models.
This next generation solution can generate <2 angstrom 3D reconstructions and produce images faster than its predecessor. These capabilities can help users of all experience levels increase productivity at a time when rapid innovation and emerging cryo-EM applications are placing increasing demands on expert microscopists.
“Using the Glacios 2 Cryo-TEM, we developed a workfl ow that enables us to determine structures of small, asymmetric complexes at high resolution and with high throughput,” said Basil Greber, principal investigator for The Greber Laboratory at the Institute of Cancer Research and beta user of the new Glacios 2 Cryo-TEM. “Uncovering such structures provides us with detailed insight into inhibitor binding and suggests a mechanism for target selectivity in cancer therapeutics that we are currently testing.”
The new system also includes Thermo Scientifi c Smart EPU Software, which contains components needed for automated data acquisition, including microscope alignment and readiness assistance, plus an open API to allow for the development of solutions tailored to a user’s needs.
For pharma and biotech companies, the Glacios 2 Cryo-TEM offers high-throughput screening and imaging to enable the routine structure generation of druggable targets, including key targets of <120 kDA in size. For labs with growing demand for cryo-EM, the Glacios 2 solution helps increase accessibility to near atomic resolution structures, while improving ease-of-use and reproducibility across a variety of workfl ows.
More information online:
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