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All-in-One Cell Imaging System
Zeiss has launched its latest all-in-one cell imaging system, the Zeiss Axiovert 5 digital, which employs artificial intelligence (AI) and automatic functions to streamline daily tasks in the cell lab. This system enhances processes, from basic research to scientific routine, by enabling more efficient and reproducible phase contrast and multichannel fluorescence imaging. Additionally, the system is designed with an intuitive operating concept, allowing even inexperienced users to produce excellent images with ease. The settings and adjustments are carried out automatically, further simplifying the imaging process.
Laboratory technicians and researchers can employ brightfield, phase, and fluorescence contrast techniques to examine cell or tissue cultures. With the press of a button, the system takes control and automatically modifies the exposure time, captures the image, switches the channel, and restarts the process. The images are saved automatically, along with all the relevant metadata and scaling information of the microscope.
The Zeiss Axiovert 5 digital system utilises artificial intelligence to optimise and streamline workflows. By using the readily available AI modules, cell counting and cell confluency can be automatically determined with a single click of a button. This allows users to quickly measure and display the percentage of cell coverage or number of cells in their Petri dish.
The ergonomic layout of the Zeiss Axiovert 5 digital is tailored to support appropriate system operation, making it an ideal fit for multi-user environments. Users can easily trigger single snaps, multi-channel image acquisitions, or video recordings with just the press of a button, requiring minimal training or prior knowledge.
More information online:
ilmt.co/PL/WMOv 59803pr@reply-direct.com
New FLIM Functionality for Enhanced Raman Microscopy
Renishaw has collaborated with Becker & Hickl GmbH, specialists in time-correlated single photon counting (TCSPC), to integrate fluorescence lifetime imaging microscopy (FLIM) functionality into its inVia™ confocal Raman microscope. This combination of Raman and FLIM in a single instrument provides pixel-to-pixel correlation between FLIM and Raman images, enhancing sample analysis across a wide range of applications.
The integrated system utilises a pulsed laser to interact with a fluorophore, and measures the fluorescence lifetime with the aid of an advanced detector and high- speed electronics. This lifetime refers to the duration that a fluorophore remains in an excited electronic state, emitting a photon as it returns to the ground state. By analyzing the decay of fluorescence, it is possible to observe changes in molecular conformation due to its sensitivity to the molecular environment. The FLIM technique has the versatility to explore various properties such as pH/ion/oxygen concentration and molecular binding.
FLIM and Raman microscopy are two complementary label-free imaging methods used in the analysis of biological cells and tissue sections. FLIM enables the visualisation of molecular interactions, and its imaging speed is at least 10 times faster than that of Raman imaging. Raman spectroscopy provides high chemical specificity and high-resolution chemical images. Combining these techniques allows for the rapid identification of regions of interest using FLIM, followed by detailed chemical and structural analysis using Raman imaging.
The FLIM data analysis is supported by the Becker & Hickl software, which offers comprehensive features such as multi-exponential, incomplete, and shifted-component analysis models. Additionally, the software includes a proprietary phasor analysis tool that can differentiate between lifetime populations. The Renishaw MS30 high-speed encoded stage is employed for both imaging modalities, enabling direct overlay of fluorescence lifetime and Raman images with high accuracy.
Renishaw Spectroscopy Business Development Manager, David Reece, commented: “We are pleased to announce a further enhancement to our award-winning inVia Raman microscope. Our collaboration with the leaders in FLIM technology, Becker and Hickl, has produced a combined FLIM Raman microscope leading to exciting new application areas.”
More information online:
ilmt.co/PL/GbzW and
ilmt.co/PL/D358 60110pr@reply-direct.com
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