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MICROSCOPY & MICROTECHNIQUES
SR Microscope Stages are More Stable with Capacitive Feedback
PI (Physik Instrumente) LP has added two higher-performance models to its successful PInano™ super resolution (SR) microscope stage series. The new models are equipped with direct-measuring capacitive sensors. This type of sensor can provide higher linearity and long-term stability than the lower cost piezoresistive sensor-equipped stages, which are still available. Capacitive sensors are also less sensitive to noise due to a high-frequency measurement principle as opposed to the DC-based sensing technique used in piezoresistive sensors. The net effect is similar to the higher quality and lower noise on FM radio channels compared to AM radio.
The large aperture accommodates microscopy accessories such as slide holders and Petri dish holders. The recessed bottom slide mount allows full rotation of the turret without in and out Z-motion, protecting valuable objectives from crashing into the stage on turret rotation. Both XY and XYZ stages are available. The low profile design of 20mm (0.8”) facilitates the integration into inverted microscopes from the four major manufacturers Leica, Nikon, Olympus and Zeiss. An optional 25x25mm positioning stage with self-clamping ultrasonic ceramic motors serves as a stable basis for the piezo scanning stage and allows imaging of large samples. The microscope stages are compatible with all major image acquisition software packages such as Micromanager™ and Metamorph™.
Features and advantages include: sensor choice - high stability capacitive or lower cost Piezoresistive sensors; 24 Bit controller with USB, Ethernet, RS-232 interface and analogue control; 200 µm XY or XYZ travel, closed-loop control for sub-nanometer precision; bottom slide mount for full turret rotation facilitates integration; many options - slide holders and petri dish holders, motor XY stage and manual stage; and longer lifetime due to ceramic encapsulated Piezo drives; software support for leading image acquisition packages.
Circle no. 464
Lens Adapter Doubles Field-of-View Throughout Zoom Range
The Model 287 Wide Angle Lens Adapter from Resolve Optics Ltd is designed to double the field of view (FOV) throughout the zoom range of any zoom camera. To date all 'off the shelf' wide angle adapters have offered a compromise between FOV, distortion and resolution. If the lens achieves the FOV and minimal distortion then the compromise will be the resolution and so on. The result often is 'milky' out of focus images that get progressively worse the nearer you get to full zoom.
For the first time a wide angle lens adapter is available that provides a true 0.5x field of view (FOV) whilst maintaining minimal distortion and high resolution throughout the zoom range of your camera.
The compromise free performance of the Model 287 was achieved by designing the adapter to work with the moving pupils of zoom lenses. Its unique design combined with the use of specialised high index glasses carefully balances the provision of a low distortion wide angle view with the higher level of correction needed at the narrow angles of view. The result is a high quality lens that has an unprecedented full 'zoom through' capability resulting in high contrast images regardless of zoom position whilst still maintaining both resolution and focus to the very edges of the image.
Aimed at the broadcast and high-end medical/industrial market each production lens adapter is individually collimated to maintain the full focus range of the prime lens. Incorporating an M37 mounting thread the Resolve Optics wide angle adapter will enable many zoom lens / camera combinations with a standard 45 degree FOV to achieve a genuine 90 degree field of view.
Circle no. 465 Enhancing Microscope Imaging and Analysis
Olympus has launched the cellSens 1.5 software platform, the latest in its line of industry-leading applications for capturing and analysing microscope images. cellSens 1.5 features Windows 7 64-bit operation. It fully integrates all the imaging and analysis capabilities of such microscopes as the newly introduced fully motorised Olympus BX63 microscope, along with all previously supported Windows 7-compatible hardware and accessories.
The platform controls both manual-encoded and motorized components for Olympus BX3 microscope systems (BX63, BX53, BX46 and BX43), including nosepieces, fluorescence illuminators, universal condensers and attenuation wheels. cellSens 1.5 is the only software that can support all of the extensive multidimensional image acquisition capabilities of the BX63.
When used with the IX81 inverted microscope, cellSens 1.5 is compatible with both Olympus Zero Drift Compensation (ZDC) and ZDC2 focusing systems, which facilitate long-term live-cell imaging studies by correcting for focal drift on demand with the ZDC and continuously in real time with the ZDC2, ensuring live images are always in focus. The new version of the software has several additional enhancements, including a streamlined method of automatically transferring data on objects in specific regions of interest from one channel to another, making it easier to count chromosomes within nuclei and handle other complex imaging and analysis challenges.
It offers enhanced processing with extensive processor- and memory-intensive features, including an optional CI Deconvolution module, which generates results in seconds, rather than minutes. Cell counting, large area stitching, fluorescence unmixing and data sharing all are fast and intuitive. Because the interface is simple to customise, users can streamline their individual workflows to provide more efficient imaging, processing, measurement, analysis and communication of their data.
Several easy-to-use interfaces are available with many preset functions; the My Functions Toolbar and Macros Toolbar can be customised so that specific hardware configurations can be turned into templates for repeated use in image capture or analysis. The system can auto-adjust hardware and image-capture settings to deliver optimal image results.
Circle no. 466
Warner Instruments is pleased to introduce its new line of German borosilicate glass coverslips in #1.0 and #1.5 thickness.
Warner Instruments has expanded its line of coverslips to include the #1.5 thickness in round, rectangular, and square sizes. The new 3mm round coverslip in #1 thickness is widely used for the cranial window technique.
High quality coverslips are essential for microscopy imaging at high resolutions (40X, 60X or 100X), and choosing the correct coverslip is critical for good performance.
Circle no. 467
New Coverslips for Microscopy Imaging
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