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Sub-Two Micron Reversed-Phase UHPLC Column
Agilent Technologies, Inc introduced the ZORBAX RRHD 300SB-C18 1.8 micron column for UHPLC systems. It is a silica reversed-phase column that can be used for higher-order reversed-phase characterisation of intact proteins and protein digests.
“Researchers are increasingly looking to UHPLC to achieve faster, high-confidence results,” said Linda Lloyd, Agilent Biocolumns Product Manager. “With these new rapid- resolution, high-definition columns, researchers can now take full advantage of the latest technology, including the 1260 bio-inert HPLCand the 1200-bar capability of the Agilent 1290 Infinity UHPLC. The column extends Agilent’s portfolio of tools for protein primary structure analysis.”
The new columns are packed with ZORBAX StableBond stationary phase, providing high stability down to pH 1, permitting use of trifluoroacetic acid and formic acid eluents with confidence. The columns are stable up to 90ºC, without compromising durability.
Circle no. 333 University of Georgia Materials Researchers Rely on GPC
“The Viscotek gel permeation chromatography system with triple detection is the most important tool in our laboratory,” said Assistant Professor Jason Locklin of the Department of Chemistry and Faculty of Engineering, University of Georgia, when referring to the Viscotek TDAmax system from Malvern Instruments.
Work carried out by Professor Locklin’s multidisciplinary team has demonstrated that adding multiple detector technologies to a gel permeation chromatography (GPC), is highly effective in the real-time investigation of polymerization kinetics and macromolecular structure for both functional polymers and surface initiated polymerisation. Compared with other GPC systems, the Viscotek TDAmax delivered more detailed viscometry and light scattering data, which was central to understanding the development, growth and behaviour of new polymer materials. “Molecular weight and molecular weight distribution (also called polydispersity) are the most important parameters in polymer science,” explained Professor Locklin. “We use the Viscotek TDAmax as a tool to monitor the progress of reactions and to evaluate the extent of control in the polymerisation reaction. When making new polymers this information is critical to our understanding and tells us if the material is forming properly and if the chemistry is working.”
Professor Locklin continued; “The real benefit of the Viscotek system is the online viscometry detection and light scattering. These capabilities provide so much more info than conventional measurements, allowing you to look for polymer structure and branching as well as molecular weight and polydispersity.” Professor Locklin’s research group includes ten PhD students focusing on chemistry, biochemical engineering, and textile science. Current projects include such products as anti-microbial coatings, biodegradable polymers, light responsive polymers, and ‘clickable’ coatings. The Viscotek TDAmax is a gel permeation chromatography (also referred to as size exclusion chromatography (SEC)) system that includes refractive index (RI), viscometry and light scattering detectors making it ideal for the characterisation of polymers.
Circle no. 334 ADVERTORIAL
BUCHI New Sepacore Flash Cartridges: High Performance and Reproducible Purifications As a complement to its flash chromatography product line, BUCHI is now introducing an extensive range of disposable flash cartridges. Sepacore®
The high quality silica packing material has been optimised to provide efficient and reproducible separations: tight particle size distribution, low metal ion content, controlled water content and neutral pH are the essential characteristics allowing high resolution and reproducible purifications. Consequently, the overall purification process can be performed faster and at a lower cost.
The full advantage of these cartridges is realised when operated with the Sepacore Flash chromatography system. This system provides an optimal scale up capability on a single system thanks to a maximal flow rate of 250mL/min. In addition, high resolution separations are possible even at high flow rates with a working pressure up to 50 bar (725 psi). Together with the prepacked cartridges, BUCHI also offers empty plastic cartridges with the unique Cartridger®
packing device and a whole
range of glass columns up to 100mm ID. The broad range of flash chromatography columns offered by BUCHI addresses almost any purification requirements. For more information visit
www.buchi.com/chromatography.
Circle no. 335 Flash cartridges are designed to allow consistently isolating
high purity compounds. The very broad range of sorbents together with the large choice of cartridge sizes provide a solution for any purification requirement. Cartridge sizes from 4g up to 330g are available with silica, C18, Amine, Cyano, Diol and SCX-2 cation exchange packing material. In addition, for challenging separations, the silica HP cartridges provide smaller particles with higher resolution capabilities. For larger scale purifications 750g and 1500g cartridges are available with silica and C18 sorbent material.
Sepacore®
for high performance purification
Flash Cartridges
■ Effi cient and reproducible purifi cations. High quality silica and optimized packing technology for high resolution separations. ■ Easy scale up from mg to hundreds of grams. From 1mg up to large scale purifi cation on the same system using disposable cartridges. ■ Suited to your chemistry. Beside silica, large choice of stationary phases available: C18, Amine, Cyano, Diol and Cation Exchange
More information on
www.buchi.com > Flash Chromatography
Circle no. 336
LAB ASIA - JULY/AUGUST - CHROMATOGRAPHY
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