15 Development of State-of-the-art Pediatric Healthcare
Porvair Filtration Group has announced that the Childrens Research Institute at the Medical College of Wisconsin has used the company's Chromatrap™ technology to develop more efficient and more precise Chromatin Immunoprecipitation (ChIP) assays. The Children's Research Institute is renowned for its state-of-the-art pediatric studies. The CRI's translational research programs are targeted to find life-saving discoveries and cures in the diseases that affect children and interventions that enhance quality of life for children and families living with chronic health conditions.
Over the last few months - Dr Ronald N Hines, Professor of Pediatrics, Pharmacology & Toxicology at the CRI has compared the efficacy of Chromatrap™ with traditional bead based ChIP technologies in his research into known regulatory elements controlling ontogeny of important human drug metabolising enzymes, such as CYP3A4. He commented: "I believe the bead-based system gave us a relatively small signal to noise ratio such that even minor deviations caused by variation in the efficiency of the bead system (for whatever reason) resulted in considerable variation in the assay."
He added: "By comparison I think the most substantial advantage of the Chromatrap system is more efficient and less variable trapping of the immunoprecipitated chromatin such that the signal to noise ratio is larger and influenced less by minor variations in trapping efficiency."
The Chromatrap™ spin column approach offers significant advantages compared to methods based on agarose or magnetic beads, which involve many steps of separation, pipetting and re-suspension. The BioVyon™ protein A discs used in Chromatrap™ spin columns have a proprietary internal structure, composed of pores that have inner surfaces specifically designed to maximise chromatin capture efficiency.
Porvair's proprietary disc base material is chemically inert, reducing incidences of non-specific binding. All chromatin capture reactions take place inside the rigid porous disc; therefore there is no need for the many repetitive stages of suspension, collection and resuspension by pipetting. Additionally no pre-blocking step is required. The Chromatrap spin column protocol allows washing steps that are fast, efficient and less prone to error and the 'flow through' characteristics of the column ensure excellent sample mixing and washing during the assay.
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High Purity Gas Analysis Solutions
The rack mounted high purity gas analyser (HPGA) is the latest chromatography solution from Ellutia. These systems have been designed with complete flexibility in its configuration, with input from major gas supply companies, to ensure they satisfy the industry requirements. This allows up to two detectors, multiple columns in an air blown oven and up to five switching valves for full multi-dimensional chromatography. The systems are designed in a 6U 19” rack module, with a secondary module containing the supply gas conditioning units. All systems come equipped with welded VCR fittings for high integrity gas connections and allowing easy servicing.
Detection limits as low as 5 ppb for trace elements, can be obtained when using a Pulsed Discharge Ionisation Detector (PDID) and low percent levels for the larger matrix components using a Thermal Conductivity Detector (TCD). Other two detector combinations available include a PDID and Flame Ionisation Detector (FID) for its large linear range for hydrocarbons and the use of two PDID detectors to gain greater flexibility at high sensitivity.
Each system possesses dual analogue output for ease of connection to integrators such as Clarity for standard integration and reporting. Ellutia can also produce and supply fully customised software to integrate with a customer existing manufacturing or control systems. The software can incorporate operations such as automated control, reporting, error checking and communication with other parts of the customers system.
The systems can analyse:
H2, Ar/02, N2 CH4, CO, CO2, C2H6, Kr, Xe and Freons In the matrix gasses
H2, Ar, O2, N2, CO2, HCl, SiH4, BCl3, N2O, CF4 and SF6 Ellutia’s Customer Support Manager, Richard Jaroszewicz said: “We have been helping a number of customers maintain old outdated analysis systems and have had many requests for us to design a system as they were not satisfied with offerings from other manufacturers. So we are delighted to be able to now offer these fantastic modern systems to fulfil the customers exacting requirements”
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