Pumps,Valves & Liquid Handling New Pipette Tips Optimise Large Volume Transfers


Beckman Coulter, Inc introduces two new Biomek P1000 Span-8 pipette tips for the company’s Biomek NXP and FXP Laboratory Automation Workstations. A clear, non-conductive P1000 Span-8 Tip and a P1000 Span-8 Wide Bore Tip are now offered in addition to the current Biomek P1000 Span-8 Conductive Tip.


The Biomek P1000 Span-8 Tip will allow users to visually inspect aspirated volumes and instil confidence during method development. Sample preparation times can be reduced by transferring a full 1mL of sample or reagent in a single pipetting action without having to split volumes. This is useful for large-volume media transfer in ‘seed and feed’ cellular applications.


The new P1000 Span-8 Wide Bore Pipette Tip offers a larger orifice, which facilitates pipetting of viscous liquids such as glycerol solutions or of samples with large particulates, which can clog standard tips. These tips are helpful in library construction and bead enrichment during sample preparation for next generation sequencing, in which viscous reagents such as the bead mixture are pipetted in large volumes.


In addition, using wide bore tips can minimise DNA shearing during nucleic- acid preparation and reduce the risk of inadvertent cell lysis when pipetting fragile cell suspensions such as hybridomas or large-cell aggregates such as embryoid bodies for stem cell research.


The current Biomek P1000 Span-8 Conductive Tip enables the liquid-level sensing capability of the Biomek system to automatically sense the sample or reagent in deep-well plates or tubes, so users do not have to specify volumes. Cross-contamination is minimised by reducing the exterior wetted surface of the tip by pipetting from the top of the liquid rather than from the bottom of the tube or plate.


To optimise workflow efficiency, validated support is provided by an import file containing tip definitions, pipetting techniques and templates; tip information in the labware library of Biomek software is automatically populated. The actual colours of the tip racks are captured in Biomek software, speeding method development by minimising potential errors in tip selection and Biomek deck set-up.


Circle no. 208 Circle no. 209 Extended Range of Wireless Vacuum Pumps


KNF Lab has launched the SC950 wireless vacuum pump system, combining fast and precise vacuum regulation with a flow rate of up to 50 l/min, plus the benefit of total remote control. The SC950 builds on the success of the acclaimed SC920 wireless pump series, offering higher flow rates while retaining the same exceptional design and extremely quiet operation. A wireless touchscreen terminal provides full control of all the pump’s operating modes and innovative functions, ensuring maximum flexibility and optimal use of laboratory space. Like the SC920, the user-friendly design of the new SC950 vacuum pump system provides straightforward operation while allowing the pump to be stored in a remote or inaccessible location, saving valuable laboratory space without the need to compromise on control. The pump can also be controlled via a PC using Microsoft Windows®


-based software. The SC950 offers a range of walk-away operating modes, including chamber evacuation, constant pressure control and


maintenance of sample vapour pressures, as well as allowing intelligent regulation of process pressures according to user-defined pressure curves. The pump’s diaphragm stabilisation system ensures high suction speeds, even at low pressures, achieving an ultimate vacuum of 2 mbar.


Circle no. 210


Enhanced Multichannel Verification System for Liquid Handling Quality Assurance


Artel has launched the new MVS Multichannel Verification System, an objective performance assessment and optimisation tool for automated liquid handlers. The MVS offers accuracy and precision information for any liquid handling device with up to 384-channels, allowing for confidence in assay data. Several recent enhancements make the MVS more flexible and easier to use, so laboratory technicians can boost quality control without impeding efficiency.


The MVS provides accuracy and precision data traceable to the International System of Units (SI) through reference standards developed and maintained by the National Institute of Standards and Technology (NIST) in an easy-to-use system that rapidly and automatically documents the volume dispense performance of each channel of an automated liquid


handler for aqueous or user defined solvents. Able to function within a volume range of 0.01 µL to 350 µL, the MVS can be fully integrated into existing automated quality control protocols and implemented by a technician of any skill level. Based on ratiometric photometry, the MVS measures the dispensed volume from any 1-, 2-, 4-, 6-, 8-, 12-, 16-, 24-, 96-, or 384-channel liquid delivery device in minutes.


With the MVS enhancements, the system can now provide volume measurements with an even higher degree of accuracy and precision. For devices utilising 384-well plates, MVS precision and accuracy tolerances have been reduced to 0.8 – 1.4% and + 2.5 – 3.5% respectively, depending on the volume range. For devices dispensing into 96-well plates, the precision and accuracy specifications have been tightened to 0.4 – 0.5% and + 2 – 3% respectively, depending on the volume range. With these new specifications, laboratories can decrease the uncertainty in their liquid handling operations for overall improvements in data quality.


In addition, the MVS can now be implemented in laboratory processes for 6-channel liquid handling devices and 4-channel liquid handlers facilitating performance assessment with greater ease and accuracy.


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