PEER-REVIEW | HAIR RESTORATION |
Figure 4 Standard photographs taken from six incidences Following local anaesthesia, the
scalp was first traumatised with a 1.5 mm microneedling roller and then injected with the PRP.
layers: an erythrocyte and granulocytes layer at the
bottom of the tube beneath the gel, a PRP layer in the middle, and a platelet-poor plasma (PPP) layer at the top of the tube. The PPP layer was drawn out (13±1 ml) and the remaining plasma (5±1 ml) was gently mixed onto the gel surface where 85%±5% of the platelets are present, creating the PRP. The remaining materials used in this study include:
■ A dedicated centrifuge ■ A 1.5mm dermaroller, (CE mark, ISO 11137 Medical, and FDA) (Figure 2) designed to make micropunctures on the surface of the scalp
■ Digital phototrichogram (Figure 3) with capillary measurement software developed by the authors’ team
■ Photographs (Figure 4) with six incidences: front, 45°, 90°, 135°, 180°, and side.
Table 1 Descriptive statistics for the samples NORMALITY TEST
AGE Seventy patients from 17 to 77 years old. The sex ratio is 39 women to 31 men.
N Age DENSITY N Density CALIBER N Caliber 70
Mean EcTyp 48.29
11.17 Chi2 6.288
Chi2 11.07
Lim
Proba <0.28
Normality ACCEPT
The above analysis demonstrated that the size of the groups chosen is acceptable and can statistically represent the population, as tested by chi2 test.
28 ❚ March 2015 |
prime-journal.com 70
Mean EcTyp 151.3
47.25 Chi2 3.494
Chi2 7.815
Lim
Proba <0.32
Normality ACCEPT
70
Mean EcTyp 44.7
14.31 Chi2 9.443
Chi2 11.07
Lim Proba Normality <0.093 ACCEPT
Figure 3 Digital measurement
Inclusion and exclusion criteria The inclusion criteria consisted of: ■ Men and women ■ 20 to 80 years old ■ Androgenetic alopecia stages II–VI of the Norwood- Hamilton scale, or 1–3 from the Ludwig scale. The exclusion criteria consisted of:
■ History of cancer ■ Use of anticoagulants or blood products ■ Scalp active skin infection ■ Haematological diseases ■ Pregnancy.
Methodology Seventy patients (31 males and 39 females) were recruited for the study. Digital measurements (tattoo mark, hair density, diameter, and ratio) and standard photographs were taken from each participant. In all, 60 cc of blood was drawn and 10 cc of PRP was processed. Following local anaesthesia, the scalp was first traumatised with a 1.5 mm microneedling roller and then injected with the PRP. Patients underwent two sessions 3 months apart. No external activation has been provided.
Schedule Before the procedure could take place, a prior consultation with haematologic analysis and informed consent was required.
First treatment on day 0 ■ Signature of informed consent ■ Photographs taken from six different angles ■ Digital phototrichogram for measurement of capillary density and the diameter of the hair in an area identified by two micro-tattoos
■ Local anaesthesia, application of the dermaroller and injection of PRP.
Second treatment on day 90 ■ Photographs taken again from the same six angles
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