EMS GRIDS & TEM SUPPORT FILMS CATALOG EDITION V TEM SUPPORT FILMS
zzz UltrAuFoil™ Holey Gold Films
These ultrastable gold supports for electron cryomicroscopy will reduce the movement of frozen specimens during imaging. This improves image contrast and quality, leading to better 3D reconstructions with less data.
During imaging at cryo-temperatures, carbon supports move, particularly at the beginning of irradiation. This movement blurs images and makes it difficult to determine the structures of small and challenging molecules.
Using UltrAuFoils™, designed at MRC’s Laboratory of Molecular Biology by Dr Christopher J. Russo and Dr Lori A. Passmore and produced by Quantifoil Micro Tools, specimen motion can be reduced significantly. (For details see: Ultrastable gold substrates for electron cryomicroscopy, Science, 2014, Vol. 346 no. 6215 pp. 1377-1380).
Characteristics of UltrAuFoil™
Thickness of Gold Foil About 500 Å Structure of Gold Foil
Ordering Information Hole
Type
R 0.6/1 Gold
R 1.2/1.3 Gold
R 2/2 Gold
Size
0.6μm 1.2μm 2μm
Period
1.6μm 2.5μm 4μm
Regular square array of micrometer-sized circular holes
All other geometries and thicknesses available upon request Grid
Cat. #
— —
Cat. # 200 Mesh 300 Mesh
Q350AR1A Q350AR13A
Q250AR2A — FREQUENTLY ASKED QUESTIONS
Do I need to modify the UltrAuFoils™ before use? No, they are ready for use when delivered. They can be made more hydrophilic using standard glow discharge and plasma systems or other gold surface treatments.
How do I focus using UltrAuFoils™? Since there is no amorphous material in the gold support structure, Thon rings cannot be used to focus. As discussed in the publication, several other options are available, but the two simplest are:
1. Turn on beam tilt wobble and minimize the image shift.
2. Look for the diffracted beams at the edge of a hole with the objective aperture removed. When the shift between the diffracted beams and the crystals of gold is minimized, the foil is in focus.
How do I correct the astigmatism? We recommend using a calibration specimen to correct the stigmation and beam tilt prior to collecting data on UlrAuFoils™
.
Can I use automated data collection methods? Yes, automated data collection has been successfully tested on UltrAuFoils™ focus.
using beam tilt to
Are UltrAuFoils™ fragile? No, they are similar or less fragile than traditional carbon foils. But if mishandled with tweezers or broken during freeze plunging, the stability of the support may be severely degraded. We recommend collecting data only from squares where the foil is uniform and intact.
Can I add a continuous film of amorphous carbon? Yes. Standard float transfer methods work fine for transferring thin films of carbon onto UltrAuFoils™
. Qty.
50/pk 50/pk 50/pk
zzzQUANTIFOIL® SiO2 Films
Resilient and extensively functionalizable, QUANTIFOIL®
SiO2 films are robust enough to
withstand extensive manual handling and FIB- milling. They are a popular choice for in situ biological imaging including cryo-electron tomography and correlated light and electron microscopy (CLEM), as well as materials science applications.
In addition, the ability to easily chemically modify SiO2 film surfaces makes them ideal when:
• Optimizing the physicochemical properties of the transmission electron microscopy supports.
• The sample support is part of the experimental protocol: for example directing the growth of a sample or to covalently attaching a biomolecule to the SiO2 surface.
Applications that may benefit from using SiO2 supports include: • On-grid cell growth for in situ imaging techniques including CLEM and cryo-FIB, due to their robustness and similarity to glass surfaces.
• The production of FIB lamallae is aided by the resilience of SiO2. • The ease of functionalization simplifies the attachment of biomolecules (for example DNA or microtubules) for controlled investigations.
• Semiconductor material characterization can take advantage of all these features.
Ordering Information Grid
Type
R 1/4 Copper Nickel Gold
R 1.2/1.3 Copper Nickel
R 1.2/20 Copper Nickel Gold
R 2/2 Copper Nickel Gold
R 2/4 Copper Nickel Gold
Hole Size
1μm 1μm 1μm
Cat. # Period 200 Mesh 5μm Q250CR-14S Cat. # 300 Mesh Q350CR-14S Cat. # 400 Mesh Q450CR-14S
5μm Q250NR-14S Q350NR-14S Q450NR-14S 5μm Q250AR-14S Q350AR-14S Q450AR-14S
Qty.
100/pk 100/pk 100/pk
1.2μm 2.5μm Q2100CR1.3S Q3100CR2.5S Q4100CR2.5S 100/pk 1.2μm 2.5μm Q2100NR1.3S Q3100NR2.5S Q4100NR2.5S 100/pk
1.2μm 21.2μm Q2100CR21.2S Q3100CR21.2S Q4100CR21.2S 100/pk 1.2μm 21.2μm Q2100NR21.2S Q3100NR21.2S Q4100NR21.2S 100/pk 1.2μm 21.2μm Q2100AR21.2S
—
2μm 2μm 2μm
2μm 2μm 2μm
4μm Q2100CR2S 4μm Q2100NR2S 4μm Q2100AR2S
6μm Q2100CR-4S
Q3100CR2S Q3100NR2S Q3100AR2S
Q3100CR-4S —
Q4100CR2S Q4100NR2S Q4100AR2S
Q4100CR-4S
6μm Q2100NR-4S Q3100NR-4S Q4100NR-4S 6μm Q2100AR-4S Q3100AR-4S Q4100AR-4S
100/pk 100/pk
100/pk 100/pk
100/pk 100/pk 100/pk
Recent examples of Cryo-ET investigations using SiO2 grids Shepherd et al. Throughput-scalable manufacturing of SARS-CoV-2 mRNA lipid nanoparticle vaccines. Proc Natl Acad Sci USA 120: e2303567120 (2023)
Winter et al. The Ebola virus VP40 matrix layer undergoes endosomal disassembly essential for membrane fusion. EMBO J 42: e113578 (2023)
Methods development for in situ structural investigation by FIB-milling and cryo-electron tomography Toro-Nahuelpan et al. Tailoring cryo-electron microscopy grids by photo- micropatterning for in-cell structural studies. Nat Methods 17: 50-54 (2020)
Lucas and Grigorieff. Quantification of gallium cryo-FIB milling damage in biological lamellae. Proc. Natl Acad. Sci. USA 120: e2301852120 (2023)
Franken et al. Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells. STAR Protocols 3: 101696 (2022)
53
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92
