EMS GRIDS & TEM SUPPORT FILMS CATALOG EDITION V SPECIMEN SUPPORT GRIDS
TECHNICAL TIP Shiny Side or Rough Side?
Retention of sections on grids during poststaining and immunocytochemical procedures frequently is of crucial impor- tance in the electron microscopy laboratory. Opinions differ regarding the side of grids most suitable for permanent adhesion.
The controversy is easily solved by examination of the surfaces involved. Grids are manufactured with a dull or rough side, and a shiny or smooth side. Epoxy sections exhibit a bumpy surface when viewed in the boat. Scanning electron microscopy images of epoxy sections without embedded material also reveal an uneven surface. Let us imagine a grid to be a single sided piece of sandpaper and the section to be a double sided piece of sandpaper. Sandpaper grips another piece of sandpaper much more readily than it does a smoothly polished metal surface. For the most secure adhesion of sections to grids, SECTIONS SHOULD BE PICKED UP ON THE ROUGH SIDE OF THE GRID.
Hildegard H. Crowley, Dept. of Biological Sciences, University of Denver, Denver, CO. 80208
TECHNICAL TIP
A Simple Method for Handling Grids
A simplified method for handling EM grids is described. This new method not only offers safety and identification of your samples but offers you improved handling, temporary storage, and identification of grids bearing ultrathin sections as well as a novel method for preparing bulk samples.
Refer to:
Gorycki, M.(1992). A Simple Method for Handling Grids. Biotechnic & Histochemistry 67/5, 313-314.
TECHNICAL TIP Reaction of Ni and Cu Grids
How do Nickel and Copper Grids react with Periodic Acid?
Periodic Acid + Ni... Ni-Periodate + H2 Periodic Acid + Cu... Cu-Periodate + H2
In this case you should use Gold Grids. EMS Grids, Molybdenum Grids
zzz EMS Grids — Square Mesh and Oval Hole Diameter: 3.05mm, Thickness: see chart
Material: Copper (Cu), Copper-Rhodium (Cu-Rh),Nickel (Ni), Gold (Au), Molybdenum (Mo)
200 mesh Type Cat#
EMS SQUARE MESH 200 mesh
EMS200-Cu EMS200-Ni
300 mesh
300 mesh Packed 100/vial
EMS200-Cu-Rh 100/vial 100/vial 50/vial 25/vial
EMS200-Au EMS200-Mo EMS300-Cu
400 mesh
EMS OVAL HOLE 2x1 mm
EMS300-Ni EMS300-Au EMS300-Mo EMS400-Cu EMS400-Ni EMS400-Au
100/vial
EMS300-Cu-Rh 100/vial 100/vial 50/vial 25/vial
100/vial 100/vial 50/vial
EMS2010-Cu EMS2010-Ni EMS2010-Au EMS2010-Mo
100/vial 100/vial 50/vial 25/vial
Pitch (µm)
125 125 125 125 125 83 83 83 83 83 62 62 62
400 mesh
TECHNICAL DATA Hole (µm)
95 95 95 95 95 58 58 58 58 58 37 37 37
Bar (µm)
35 35 35 35 35 25 25 25 25 25 25 25 25
2x1 mm Thickness
Up to 27µm, +/-5µm Up to 27µm, +/-5µm Up to 27µm, +/-5µm 11µm, +/-2µm 25µm, +/-4µm
Up to 19µm, +/-5µm Up to 19µm, +/-5µm Up to 19µm, +/-5µm 10µm, +/-2µm 25µm, +/-4µm
Up to 19µm, +/-5µm Up to 19µm, +/-5µm 9µm, +/-2µm
— 2000x1000 — 27µm, +/-5µm — 2000x1000 — 27µm, +/-5µm — 2000x1000 — 27 micron — 2000x1000 — 25µm, +/-4µm
zzzTEM Specimen Supports in Molybdenum
We have extended our range of TEM grid materials to include four types, which are now available in Molybdenum. The new products are manufactured using a process known as chemical ‘milling’ (etching) instead of the more familiar technique of ‘electroforming’ (deposition) that is used in the manufacture of copper, nickel and gold products. Molybdenum is used principally in applications where it’s high temperature, hardness, expansion of coefficient and corrosion resistance characteristics are considered important. The material which is used has a purity of 99.9%.
Overall Diameter: 3.05mm Rim Width:
Center Mark: Rim Mark:
Molybdenum: Symbol:
Atomic number: Melting point: Boiling point: Density:
Type
200 mesh 100 mesh 2 x 1
1000 micron 2
Mo 42
2617.0°C (2890.15°K, 4742.6°F) 4612.0°C (4885.15°K, 8333.6°F) 10.22 g/cm3
Cat#
G200-Mo G100-Mo G2010-Mo G1000-Mo
Packed 25/vial 25/vial 25/vial 25/vial
Pitch (µm) 125 250
90 205
2 x 1 mm 1000 µm
G200-Mo, G100-Mo: 0.225mm G200-Mo, G100-Mo: Yes G200-Mo, G100-Mo: Yes
G2010-Mo 2x1Grid
TECHNICAL DATA Hole (µm)
G1000-Mo 1000 Micron Grid
G200-Mo 200# Grid
G100-Mo 100# Grid
Bar (µm) 35 45
Thickness 25µm 25µm 50µm 50µm
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92
