EMS GRIDS & TEM SUPPORT FILMS CATALOG EDITION V TEM SUPPORT FILMS
C-flat™ Family of Holey Carbon, Gold Grids for S/TEM and Cryo-TEM (continued) C-flat™ (continued)
The premier holey carbon grid for cryo-transmission electron microscopy
zzz CD-flat™ for Automated S/TEM Imaging and Metrology
The C-flat™ advantage in a new pattern
Above: Cryo-ET using C-Flat 2/2 hole pattern on Covid 19 Spike Protein. Ke, Z., Oton, J., Qu, K. et al. Structures and distributions of SARS-CoV-2 spike proteins on intact virions. Nature (2020).
https://doi.org/10.1038/s41586-020-2665-2
Automated TEM: C-flat™
provides a regular array of analysis sites compatible with automated data collection software such as Leginon. This compatibility, in combination with the more uniform ice thickness and particle distribution reported by numerous researchers, results in more high-quality target sites per grid.
Publications using C-flat™:
Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction. Proceedings of the National Academy of Sciences, Volume 105, Number 6, pp. 1867-1872, 2008. X. Zhang, E. Settembre, C. Xu, P. R. Dormitzer, R. Bellamy, S. C. Harrison, and N. Grigorieff
Preparation of macromolecular complexes for cryo-electron microscopy. Nature Protocols, Volume 2, pp. 3239 - 3246, 2007. R. A. Grassucci, D. J. Taylor, and J. Frank
Segrosome structure revealed by a complex of ParR with centromere DNA. Nature, Volume 450, pp. 1268-1271, 2007. M. A. Schumacher, T. C. Glover, A. J. Brzoska, S. O. Jensen, T. D. Dunham, R. A. Skurray and N. Firth
Automation of random canonical tilt and orthogonal tilt data collection using feature-based correlation. Journal of Structural Biology, Volume 159, Issue 3, pp. 335-346, September 2007. C. Yoshioka, J. Pulokas, D. Fellmann, C. S. Potter, R. A. Milligan and B. Carragher
Automated cryoEM data acquisition and analysis of 284 742 particles of GroEL. Journal of Structural Biology, Volume 155, Issue 3, pp. 470-481, September 2006. S. M. Stagg, G. C. Lander, J. Pulokas, D.s Fellmann, A. Cheng, J. D. Quispe, S. P. Mallick, R. M. Avila, B. Carragher and C. S. Potter
Contamination buildup limit throughput for automated cryoEM? Journal of Structural Biology, Volume 154, Issue 3, pp. 303-311, June 2006. A. Cheng, D. Fellmann, J. Pulokas, C. S. Potter and B. Carragher
Articles
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Daniel Wrapp, Nianshuang Wang, Kizzmekia S. Corbett, Jory A. Goldsmith, Ching-Lin Hsieh, Olubukola Abiona, Barney S. Graham, Jason S. McLellan, 2020. Science, 13 Mar 2020: Vol. 367, Issue 6483, pp. 1260-1263, DOI: 10.1126/
science.abb2507
An improved holey carbon film for cryo-electron microscopy. Quispe J, Damiano J, Mick SE, Nackashi DP, Fellmann D, Ajero TG, Carragher B, Potter CS, (2007). Microscopy and microanalysis, 13(5), 365-371.
Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: electrostatic charging for section attachment and implementation of an anti-contamination glove box. Pierson J, Fernández JJ, Bos E, Amini S, Gnaegi H, Vos M, Bel B, Adolfsen F, Carrascosa JL, Peters PJ., J Struct Biol. 2010 Feb;169(2): 219-25. Epub 2009 Oct 12.
Specifications Grid Material
Mesh Size O.D.
Film Material Film Thickness Hole Pattern
Copper
200, 300 Mesh 3.05mm
Holey Carbon 40nm 8/2
(8 um diameter, 2 µ spacing between holes)
Ordering Information Product Code
Cat. #
CD-flat™ — the premier ultra-flat holey carbon grid is ready to use with no additional cleaning or handling steps, and is now available, featuring a NEW 8/2 hole pattern. CD-flat is designed for CD-TEM metrology of large specimens, like 3D NAND FIB lamella.
Qty.
CDF-8/2-2Cu-T-50 CDFT822-50 50/pk CDF-8/2-3Cu-T-50 CDFT823-50 50/pk
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