EMS GRIDS & TEM SUPPORT FILMS CATALOG EDITION IV TEM SUPPORT FILMS


The C-flat™ Family


Holey Carbon, Gold Grids for S/TEM and Cryo-TEM...


now by EMS.


Electron Microscopy Sciences is pleased to announce the acquisition of the C-flat™, Au-flat™, and CD-flat™ product lines, which can be purchased directly from EMS effective immediately.


We are excited to continue to offer and expand on the C-flat line and look forward to working with all our current and new customers in the years ahead.


The C-flat™ Advantage


C-flat™


is an ultra-flat, holey carbon- coated TEM support grid for transmission electron microscopy (TEM). Unlike competing holey carbon films, C-flat™


is manufactured without


plastics, so it is clean upon arrival and the user has no residue to contend with.


C-flat™


leads to better data sets. Made with patented technology, C-flat™ provides an ultra-flat surface that results in better particle dispersion and more uniform ice thickness. Patterning is done using deep-UV projection lithography, ensuring the most accurate and consistent hole shapes and sizes down to submicron features. The precise methods by which C-flat™


is manufactured


eliminate artifacts such as excess carbon and edges around holes.


C-flat™ C-flat™


is affordable


is available in 25, 50, and 100 packs at a per-grid price less than competing products.


With 54 varieties, there is always a C-flat™ product optimized for your needs.


Below: 250,000 particles of GroEL in 24 hours. Image Courtesy of Scott Stagg and Mike Pique NRAMM, The Scripps Research Institute (data acquired on CF-2/2-4C)


Applications


The perfect choice for... • Single particle analysis • Cryo electron tomography • Automated TEM workflow


C-flat™


holey carbon grids provide the ideal specimen support to achieve high resolution data in cryo-TEM making them an ideal choice for single particle analysis, cryo electron tomography and automated TEM analysis.


Cryo-electron tomography (cryoET) and Single Particle Analysis (SPA): Numerous researchers have reported that the ultra-flat surface of C-flat™


leads to even ice


thickness and uniform particle distribution within the hole areas. This optimal particle distribution results in superior data being collected as compared with other holey support films. 2µm hole sizes are standard but custom


40


Hole Diameter Hole Spacing


TEM Grid Mesh


Insert of Carbon Film


TEM Grid Material


2.9 Angstrom Resolution Cryo-EM 3-D Reconstruction of Close-packed PCV2 Virus-like Particles PDB ID: 3JCI Liu, Z., Guo, F., Wang, F., Li, T.C., Jiang, W. (2016) Structure 24: 319-328


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