EMS GRIDS & TEM SUPPORT FILMS CATALOG EDITION V TEM SUPPORT FILMS
C-flat™ Family of Holey Carbon, Gold Grids for S/TEM and Cryo-TEM (continued)
zzz Au-flat™ for High Resolution Imaging Gold foil sample supports for Cryo-EM
Au-flat is an ultrastable Cryo-EM sample support with a 45nm holey Gold alloy film on 3 mm gold mesh grids. Au-Flat is a derivative of our patented C-flat product.
What varieties of Au-flat are available? Au-flat is offered in three configurations:
• 0.6 μm/1.0 μm hole pattern on 300 mesh Gold grids • 1.2 μm/1.3 μm hole pattern on 300 mesh Gold grids • 2μm/2μm Hole pattern on 200 mesh Gold grids
What are the benefits of Au-flat over Holey Carbon Supports like C-flat?
Better reconstructions with less data Au-flat significantly reduces beam-induced motion during imaging compared to carbon films, improving image quality and resolution.
Biocompatible
Specifications Grid Material
Mesh Size O.D.
Film Material Film Thickness
Hole Patterns 0.6/1.0
1.2/1.3 2/2
Au-flat features a holey Au/Pd film on a gold mesh grid, so it’s chemically inert and biologically compatible.
Durable Gold
200, 300 Mesh 3.05mm Gold Alloy 45nm
0.6 um dia., 1.0 µ spacing
1.2 um dia., 1.3 µ spacing
2 um dia. 2 µ spacing
Au-flat’s film is significantly stronger than carbon films and is more capable of surviving the Cryo-EM workflow including tweezer handling, glow discharge, blotting, auto-grid loading and plunge freezing.
What are the benefits of Au-Flat over other Gold Foil TEM Grids? No Mistakes
The lighter color of the Au/Pd alloy film compared to the underlying gold mesh grid makes it easy to identify the “holey” side of the grid during sample prep. This ensures the sample is deposited on the correct side and that the grid is loaded and imaged in the correct orientation.
Stronger
Au-flat is produced with gold mesh grids that are about 6 microns thicker than typical Cryo-EM grids. This makes the grids stiffer and less likely to bend or deform. The increased thickness is fully compatible with side-entry holders as well as auto-loaders.
Ordering Information Product Code
GF-0.6/1.0-3Au-45nm-50 GF-0.6/1.0-3Au-45nm-5
GF-1.2/1.3-3Au-45nm-50 GF-1.2/1.3-3Au-45nm-5
GF-2/2-2Au-45nm-50 GF-2/2-2Au-45nm-50
100 nm Accurate defocus
The smaller grain size of the gold film provides more features to focus on, leading to more accurate defocus during data collection.
44 Cat. #
AUFT306-50 AUFT306-05
AUFT313-50 AUFT313-05
AUFT222-50 AUFT222-05
Hole Size
0.6 µm 0.6 µm
1.2 µm 1.2 µm
2 µm 2 µm
Spacing 1.0 µm 1.0 µm
1.3 µm 1.3 µm
2 µm 2 µm
Hole
TEM TEM Mesh Grid
300 300
300 300
200 200
Au Au
Au Au
Au Au
Qty.
50/pk 5/pk
50/pk 5/pk
50/pk 5/pk
The lighter color of the Au-Pd film compared to the underlying gold mesh grid makes it easy to identify the film side of the grid during sample preparation.
Au/Pd Foil Side Gold Mesh Side Uniform vitreous ice across the grid
Au-flat uses new, ultra-flat precision gold grids which create a more planar surface. The result is more uniform thin ice across the entire surface, including areas near the grid bars.
10 μm
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