EMS GRIDS & TEM SUPPORT FILMS CATALOG EDITION V TEM SUPPORT FILMS
QUANTIFOIL® Holey Carbon Films (continued) TECHNICAL TIP Additional Ultrathin Continuous Carbon Layer
An ultrathin (2-3 nm) carbon layer applied to grids is widely recommended to improve particle distribution1,2,3
improve data quality when applied to both QUANTIFOIL® 4 Films and UltrAuFoil® 5
Gold Supports.
Many biomolecules have a strong affinity for carbon, and a number of factors contribute to the improved specimen quality:
z Increased numbers of particles due to adsorption of biomolecules onto carbon prior to blotting.
z Improved particle distribution due to interaction with carbon surface across the hole.
z Reduction in the number of particles adopting a preferred orientation.
An additional carbon layer can be added to most QUANTIFOIL holey carbon film support. The most popular thickness is 2 nm.
and has been shown to Holey Carbon
zzzQUANTIFOIL® with Circular Holes (continued) QUANTIFOIL® R 2/4 may be preferred over R 2/2,
when an increased tolerance with respect to the position of beam, and a larger beam diameter are desired, such as in the case of automated image acquisition.
Grid Type Copper
Period: 6μm Nickel Gold
Cat. # 200 Mesh Q210CR-4
Hole Size: 2μm Q225-CR4 Q250-CR4 Q2100CR-4 Q210NR-4
Hole Size: 2μm Q225-NR4 Q250-NR4 Q2100NR-4 Q210AR-4
Period: 6μm
Hole Size: 2μm Q225-AR4 Q250-AR4 Q2100AR-4
Period: 6μm
QUANTIFOIL® Copper
Period: 6μm Gold Period: 6μm
Hole Size: 2μm Q225CR4-2nm Q250CR4-2nm
Cat. # 300 Mesh Q310CR-4 Q325CR-4 Q350CR-4 Q3100CR-4 Q310NR-4 Q325NR-4 Q350NR-4 Q3100NR-4 Q310AR-4 Q325AR-4 Q350AR-4 Q3100AR-4
R 2/4 with Ultrathin Carbon
Q210CR-4-2nm Q310CR-4-2nm Q410CR-4-2nm 10/pk Q325CR-4-2nm Q425CR-4-2nm 25/pk Q350CR-4-2nm Q450CR-4-2nm 50/pk
Hole Size: 2μm Q225AR4-2nm Q250AR4-2nm
Q2100CR4-2nm Q3100CR-4-2nm Q4100CR-4-2nm 100/pk Q210AR-4-2nm Q310AR-4-2nm Q410AR-4-2nm 10/pk Q325AR-4-2nm Q425AR-4-2nm 25/pk Q350AR-4-2nm Q450AR-4-2nm 50/pk
Q2100AR-4-2nm Q3100AR-4-2nm Q4100AR-4-2nm 100/pk
QUANTIFOIL® R 3/3 Hole size is 3μ. Space between holes is 3μ. Center to center is 6μ.
Grid Type Copper
Period: 6μm Nickel Gold
Cat. # 200 Mesh Q210CR3
Hole Size: 3μm Q225CR3 Q250CR3 Q2100CR3 Q210NR3
Hole Size: 3μm Q225NR3 Q250NR3 Q2100NR3 Q210AR3
Period: 6μm
Hole Size: 3μm Q225AR3 Q250AR3 Q2100AR3
Period: 6μm
Shown above is the structure of Photorhabdus luminescens TcdA1, a large multi-subunit complex toxin, with activity against insects, whose structure was determined using images collected on QUANTIFOIL R 2/1 grids with a 2 nm additional carbon layer6
. References
1. Passmore, LA and Russo, CJ. Methods Enzymol. 579: 51-86 (2016). 2. Drulyte, I et al. Acta Cryst D74: 560-571 (2018). 3. Thompson, RF et al. Methods 100: 3-15 (2016). 4. Rawson, S et al. Structure 23: 461-471 (2015). 5. Russo, CJ and Passmore, LA. J. Struct. Biol. 193:33-44 (2016). 6. Leidreiter, F et al. Sci. Adv. 5: eaax6497 (2019).
48
Cat. # 300 Mesh Q310CR3 Q325CR3 Q350CR3 Q3100CR3 Q310NR3 Q325NR3 Q350NR3 Q3100NR3 Q310AR3 Q325AR3 Q350AR3 Q3100AR3
Cat. # 400 Mesh Q410CR3 Q425CR3 Q450CR3 Q4100CR3 Q410NR3 Q425NR3 Q450NR3 Q4100NR3 Q410AR3 Q425AR3 Q450AR3 Q4100AR3
QUANTIFOIL® R 3/5 Hole size is 3μ. Space between holes is 5μ. Center to center is 8μ.
Grid Type Copper
Period: 8μm Nickel Gold
Cat. # 200 Mesh Q210CR5
Hole Size: 3μm Q225CR5 Q250CR5 Q2100CR5 Q210NR5
Hole Size: 3μm Q225NR5 Q250NR5 Q2100NR5 Q210AR5
Period: 8μm
Hole Size: 3μm Q225AR5 Q250AR5 Q2100AR5
Period: 8μm
Cat. # 300 Mesh Q310CR5 Q325CR5 Q350CR5 Q3100CR5 Q310NR5 Q325NR5 Q350NR5 Q3100NR5 Q310AR5 Q325AR5 Q350AR5 Q3100AR5
Cat. # 400 Mesh Q410CR5 Q425CR5 Q450CR5 Q4100CR5 Q410NR5 Q425NR5 Q450NR5 Q4100NR5 Q410AR5 Q425AR5 Q450AR5 Q4100AR5
Qty.
10/pk 25/pk 50/pk
100/pk 10/pk 25/pk 50/pk
100/pk 10/pk 25/pk 50/pk
100/pk Qty.
10/pk 25/pk 50/pk
100/pk 10/pk 25/pk 50/pk
100/pk 10/pk 25/pk 50/pk
100/pk
Cat. # 400 Mesh Q410CR-4 Q425CR-4 Q450CR-4 Q4100CR-4 Q410NR-4 Q425NR-4 Q450NR-4 Q4100NR-4 Q410AR-4 Q425AR-4 Q450AR-4 Q4100AR-4
Qty.
10/pk 25/pk 50/pk
100/pk 10/pk 25/pk 50/pk
100/pk 10/pk 25/pk 50/pk
100/pk
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