EMS GRIDS & TEM SUPPORT FILMS CATALOG EDITION V TEM SUPPORT FILMS
NEW... HexAuFoil® Ultra-Small Hole Gold Support Grids
Improve every image with this revolutionary, optimized, ultra-small hole support geometry.
What is unique to HexAuFoil?
With it’s revolutionary small-hole, all-gold design, it allows for virtually movement-free imaging creating zero dose reconstructions for the first time. HexAuFoil’s unique high-density, hexagonal design, and improved stability mean less re-focusing and faster data collections.
HexAuFoil is ideal for cryo-EM scientists in industry or academia looking to maximize output of high-quality reconstructions for SBDD and research.
Over the last decade, the increasing use of cryo-EM has driven a demand for ever higher quality data. One source of information loss and map quality
Relative Ice Thickness
Relative ice thickness plot for apoferritin data collected on a HexAuFoil grid. (Plot produced using CryoSPARC).
reduction is particle movement. While data processing can reduce the effect of this issue, novel sample preparation techniques can eliminate it. Investigations by the team led by Dr Chris Russo at MRC Laboratory of Molecular Biology, Cambridge were able to conclude that a key factor was reducing the size of the holes on a grid to 300nm: roughly 10 times the desired 30nm ice thickness. Delivering holes this small required the development of a completely new manufacturing process, and led to the launch of HexAuFoil ultra-small hole gold supports: invented at the MRC LMB, and commercialized by Quantifoil, part of SPT Labtech.
CTF Fit Resolution (A)
CTF plot for apoferritin data collected on a HexAuFoil grid. (Plot produced using CryoSPARC).
The new process not only delivered movement-free imaging (as illustrated below), but also a step-change in grid specification, with hole diameter variation as little as 10nm, and grid bar and thickness similarly controlled. The unrivaled consistency of HexAuFoil leads to consistently thin ice: ice variation across a dataset can be as little as 1%, at a thickness of 35nm +/- 7nm. Other processing parameters show similar improved consistency, resulting in simpler data processing. Taken together, these benefits enable high quality, high resolution datasets to be collected with as few as 400 movies, despite the smaller holes meaning fewer particles per movie. Finally, the consistent ice means that a much larger number of holes in each hexagon can be collected, and this combined with the unique densely-packed hexagonal design results in data collection times can be as much as halved.
With better data, faster, in a small number of images, HexAuFoil delivers better data every image.
Ordering Information Note: for sale in the United States only. Grid Type
Hole Size
0.3μm 0.3μm
Period
R 0.3/0.3 Hex on Gold Hex 400 Mesh Gold Gold
0.3μm 0.3μm
Cat. #
Q410AR03HEX Q450AR03HEX
Comparison of particle trajectories for data collected (on the left) on UltrAuFoil and (on the right) HexAuFoil. (Plot produced using CryoSPARC) Qty.
10/pk 50/pk
UltrAuFoil R1.2/1.3 52
HexAuFoil
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