EMS GRIDS & TEM SUPPORT FILMS CATALOG EDITION V TEM SUPPORT FILMS
QUANTIFOIL® Holey Carbon Films
Overview QUANTIFOIL®
is a perforated support foil with pre-defined hole size, shape and arrangement. It has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) microscopy when compared with conventional holey film.
QUANTIFOIL® QUANTIFOIL®
is offered with circular and square, orthogonal arranged holes. Films with different hole sizes and bar widths are available. Carbon is the standard foil material.
is a superior quality of holey
carbon film, which facilitates the use of automation in TEM. (e.g. The National Resource for Automated Molecular Microscopy, at the Scripps Research Institute, has developed a system, called Leginon, for automatically acquiring images from a transmission electron microscope).
The surface properties of QUANTIFOIL® holey carbon support film, especially the wetting properties, may have to be adapted according to one’s particular requirements. Untreated aging QUANTIFOIL®
tends to be hydrophobic.
Hydrophillicity of the foil can be achieved by glow discharging in residual air or by metal coating.
QUANTIFOIL® in low-energy electron point
source (LEEPS) microscopy. QUANTIFOIL® with a regular pattern is required in order to be able to distinguish an object, which is spanned over a hole. An object cannot be discriminated from the support in the case of conventional holey support film. (H.W. Fink & C. Schonenberger, University of Basel, used QUANTIFOIL®
for the measurement of electrical current through DNA molecules.)
The foil is ~12 nm thick and mounted on either copper, nickel or gold grids with either square or round holes of different sizes.
Holey films with 2μ round holes are used at magnifications between 30,000x and 40,000x.
zzzQUANTIFOIL® QUANTIFOIL®
with circular holes is used in
cryoelectron tomographic reconstruction. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness. The hole size chosen depends on the
magnification used, and on whether or not one wishes to include support film in the image.
Assessment of the image quality is easier when foil is included in the picture, because the power spectrum of a foil is stronger than that of unsupported ice.
with Circular Holes QUANTIFOIL®
Type
R 0.6/1 R 1/2 R 1/4
R 1.2/1.3 R 1.2/20 R 2/1 R 2/2 R 2/4 R 3/3 R 3/5
R 3.5/1 R 5/10 R 5/20 R 6/6.5 R 6/100 R 10/5
R 10/10 R 10/20 R 17/5
Hole size (µm)
0.6 1.0 1.0 1.2 1.2 2.0 2.0 2.0 3.0 3.0 3.5 5.0 5.0 6.0 6.0
10.0 10.0 10.0 17.5
Spacing (µm)
1.0 2.0 4.0 1.3
20.0 1.0 2.0 4.0 3.0 5.0 1.0
10.0 20.0 6.5
100.0 5.0
10.0 20.0 5.0
QUANTIFOIL® R 0.6/1 Hole size is 0.6μ. Space between
holes is 1μ. Center to center is 1.6μ (hole size may be as large as 1μ).
Grid Type
Hole Size
Cat. # Period 200 Mesh Cat. # 300 Mesh Cat. # 400 Mesh
Copper 0.6μm 1.6μm Q210CR-06 Q310CR-06 Q410CR-06 Q220CR-06 Q320CR-06 Q420CR-06 Q225CR-06 Q325CR-06 Q425CR-06
Nickel Qty.
10/pk 25/pk 50/pk
Q250CR-06 Q350CR-06 Q450CR-06 100/pk
0.6μm 1.6μm Q210NR-06 Q310NR-06 Q410NR-06 10/pk Q220NR-06 Q320NR-06 Q420NR-06 25/pk Q225NR-06 Q325NR-06 Q425NR-06 50/pk Q250NR-06 Q350NR-06 Q450NR-06 100/pk
Gold
0.6μm 1.6μm Q210AR-06 Q310AR-06 Q410AR-06 10/pk Q220AR-06 Q320AR-06 Q420AR-06 25/pk Q225AR-06 Q325AR-06 Q425AR-06 50/pk Q250AR-06 Q350AR-06 Q450AR-06 100/pk
QUANTIFOIL® R 1/2 Hole size is 1μ. Space between holes is 2μ. Center to center is 3μ
Grid Type
Copper Nickel
Hole Size
1μm 1μm Cat. # Period 200 Mesh Cat. # 300 Mesh Cat. # 400 Mesh
3μm Q210CR-12 Q310CR-12 Q410CR-12 Q225CR-12 Q325CR-12 Q425CR-12 Q250CR-12 Q350CR-12 Q450CR-12
Qty.
10/pk 25/pk 50/pk
Q2100CR-12 Q3100CR-12 Q4100CR-12 100/pk
3μm Q210NR-12 Q310NR-12 Q410NR-12 10/pk Q225NR-12 Q325NR-12 Q425NR-12
25/pk
Q250NR-12 Q350NR-12 Q450NR-12 50/pk Q2100NR-12 Q3100NR-12 Q4100NR-12 100/pk
Gold 1μm
3μm Q210AR-12 Q310AR-12 Q410AR-12 10/pk Q225AR-12 Q325AR-12 Q425AR-12
25/pk
Q250AR-12 Q350AR-12 Q450AR-12 50/pk Q2100AR-12 Q3100AR-12 Q4100AR-12 100/pk
46
Period (µm)
1.6 3.0 5.0 2.5
21.2 3.0 4.0 6.0 6.0 8.0 4.5
15.0 25.0 12.5
106.0 15.0 20.0 30.0 22.5
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