46 ANTI-POLLUTION
Its expression decreases during the cellular senescence caused by aging, thus Lamin B1 is an interesting aging marker. However, its overexpression is harmful for
cells. Indeed, the overexpression of Lamin B1 increases the rate of proliferation and delays the appearance of the cells senescence leading to the stop of the cellular cycle in G1/ S phases.4
In summary, this protein expression
should be balanced to ensure homeostasis of the metabolism and maintain cell survival. Among the explants exposed to UVA,
we observe a decrease in the expression of Lamin B1 (-20%). This decrease is not observed in the presence of the citrus limon fruit extract active, which maintains the physiological level of Lamin B1 despite external stress (Figure 10). Thus, the citrus limon fruit extract active may protect the cells from senescence.
In vivo tests: roughness improvement The volunteers’ skin texture was analysed via VISIA-CR Stability at Day 0 and Day 28 of the study. Each subject was asked to clean their face with soap then apply the day/night cream to the entire face, twice a day, for the duration of the study. With VISIA 6th generation, numeric
pictures of the volunteers’ faces were obtained, and the skin texture was analysed. After 28 days of twice daily use, the citrus limon fruit extract active induced a significant reduction of the skin’s roughness on 76% of the volunteers. The average score reduction is -16% with a maximum of -43% (Figure 11).
Ex vivo tests: protein quantification in skin explants exposed to UVA (Elastin & MMP1) Stress applied to the skin decreases the expression of Elastin within the explants. Indeed, the intensity of the marking decreases (-9%) and the elastic fibres appear to be shortened. In the presence of citrus limon
Figure 13: Quantification of elastin labelled by immunofluorescence in skin explants after exposure UVA and treatment with citrus limon fruit extract active (2%)
fruit extract active, the elastic fibres are not affected and present the same aspect as the basal state. In addition, we detect a revival of Elastin expression in the tissue with citrus limon fruit extract active treatment (+22%) (Figure 12).
MMP1 is an interstitial protease produced in the skin and responsible for the degradation of Collagene I, III and VII. Exposure to UVA increases the production of MMP1 within the tissue (+17%) leading to the degradation of
collagens in the dermis and the basal lamina. In the presence of citrus limon fruit extract
active, we observe almost no activation of MMP1 since the expression is inhibited by 80% (Figure 13). The citrus limon fruit extract active thus preserved the integrity of dermal collagen fibres in conditions of external stress.
Conclusion In an urban environment, under conditions of polluting stress and sun exposure, Vitalisis allows the skin to adapt on several levels to continue to live in harmony with this environment (Figure 14). This new active ingredient simply preserves skin vitality in everyday life.
Figure 12: Quantification of elastin labelled by immunofluorescence in skin explants after exposure UVA and treatment with citrus limon fruit extract active (2%)
UNTREATED UVA
UVA + CITRUS LIMON FRUIT EXTRACT ACTIVE
UNTREATED UVA
UVA + CITRUS LIMON FRUIT EXTRACT ACTIVE
PC
References 1. United Nations. Policies on spatial distribution and urbanization have broad impacts on sustainable development. December 2020.
https://www.un.org/development/desa/pd/ sites/
www.un.org.development.desa.pd/files/ undes_pd_2020_popfacts_urbanization_ policies.pdf
2. Kugel S, Mostoslavsky R. Chromatin and beyond: the multitasking roles for SIRT6, Trends Biochem. Sci. 2014
3. Engel A, Niederer F, Klein K, Gay RE, Kyburz D, Camici GG, Gay S, Ospelt C. SIRT6 regulates the cigarette smoke-induced signalling in rheumatoid arthritis synovial fibroblasts. J Mol Med. 2014
Figure 14: The citrus limon fruit extract active mechanism of action PERSONAL CARE July 2024
4. Freund A, Laberge R, Demaria M, Campisis J. Lamin B1 loss is a senescence-associated biomarker. Molecular Biology of the Cell. 2012
www.personalcaremagazine.com
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