28 FUNCTIONAL INGREDIENTS
Figure 6: Ruby Bio RubyGL-EM1 uniform droplet sizes dispersed in oil in water emulsion produced by our bio emulsifier 100X magnification
in formulations, as they are key to survival of the natural yeast. Ruby Bio is capturing the best performers in nature and harnessing these qualities into raw material ingredients.
Figure 4: Oil production globally by vegetable oil source directly related to consumption4
Biology meets chemistry for an improved world Another approach to this tunable platform is derivatization to create other functionalities. The yeast generates a simplistic base molecule with site-specific reactive groups, which provides opportunities for derivatization using chemistry to enhance and adjust properties of the original base molecule. For example, simple attachment of the
anionic group to the original molecule shows powerful fragrance solubility properties shown in Figure 8, while maintaining a 95% bio-based naturally fermented ingredient. Other known glycolipids have not ventured
towards such derivatization, potentially due to absence of unique desirable reactive groups but Ruby’s glycolipid base structure allows for a new class of surfactants and opportunities to arise from our simple fermentation. These derivatizations can provide bio-based, biodegradable solutions for personal care and other large volume industries such as oil & gas and home care. Utilizing both nature and chemistry unlocks
Figure 5: Oil yield by vegetable oil source per measure of equal land use demonstrating palm oil is clearly the most efficient use of land for oil production5
meaningful use in formulations in concert with other components. The CMC is the dose or level of surfactant at which the surfactant delivers optimum surface tension reduction, and surface tension cannot further reduce beyond this level. Therefore, a surfactant with an extremely
low CMC improves efficiency of the system. As demonstrated in Figure 7, the glycolipid bio-emulsifier can reduce the CMC of the primary surfactant at an impressively low dose, improving efficiency of the system and delivering top performance. Since the yeast needs the glycolipid to
perform various tasks beyond emulsification, we see great wetting and dispersing properties of our glycolipid. In nature, the yeast will experience minerals, dust, and various other external particles that the yeast has evolved the glycolipid to manage. This is useful for formulations.
PERSONAL CARE July 2024 Minerals such as titanium oxide, zinc oxide
and pigments are used in cosmetic products and need proper dispersing and wetting to provide optimal performance. The challenge addressed by the yeast’s glycolipids in nature is the same as the challenge presented by minerals in formulations. Solid pigments added to the liquid medium will agglomerate, often entrapping air at the surface of the pigment agglomeration. For proper wetting to occur, the air
entrapped in the pigment needs wetting with a liquid medium. The use of a wetting agent, such as the glycolipid, speeds up the process and ensures complete wetting of the pigment and removal of the trapped air. Ruby’s glycolipid provides excellent
dispersing and wetting, with the same level of performance it has provided in nature for the yeast. These dispersing and wetting characteristics are key to excellent performance
not only superior performance but also improved sustainability. Leveraging the initial molecule with various derivatizations creates multiple biosurfactant solutions from the same fermentation process while reducing cost, increasing demand, and driving economies of scale.
Ruby Bio at scale with sustainability in mind Ruby Bio’s innovative fermentation platform harnesses highly efficient natural strains to produce novel glycolipids. We screened natural variants for robust glycolipid production to deliver optimal output naturally under specific conditions. Focusing on natural efficiency unlocks
commercially viable fermentation titers without needing to engineer the production strain. This allows the process to scale quickly and cost-effectively. The platform does employ genetic engineering efforts, but they are focused on pioneering new glycolipids, offering more solutions to our customers’ needs in the future. Our initial glycolipid is a natural non-GMO
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