Sample Preparation & Processing


Fast, Cost-Effective Products for Extracellular Vesicle Isolation


AMSBIO announce a new range of different isolation products for fast, scalable, and reproducible purification of Extracellular Vesicles (EV), including exosomes, as well as addressing EV heterogeneity and high throughput solutions for biomarker discovery.


Traditionally ultracentrifugation has been the favoured method for EV isolation or separation. Though it is still widely used, ultracentrifugation not only tends to alter the vesicle shape and functionality but is time-consuming and requires expensive equipment. The EV isolation product range from AMSBIO sets a new gold standard for researchers who can now select an optimised purification solution based upon their downstream needs.


For researchers looking for a method that recovers the highest amount of extracellular material, regardless of its vesicular or non-vesicular nature, AMSBIO has introduced the ExoPure Kit. This high recovery/low specificity precipitation kit uses polyethylene glycol (PEG) to separate/ isolate exosomes from your sample, requiring a short incubation time of just one hour.


Tangential flow filtration (TFF) is a rapid and efficient method, widely used for separation and purification of biomolecules that has now been successfully applied to isolate and separate extracellular vesicles. For labs that need a method that recovers mixed EVs along with some free proteins, AMSBIO has introduced three different typologies of TFF filters suitable for EV purification, EV concentration and EV size-based separation. The key benefits of these TFF products are that they are washable / reusable, enable fast concentration of diluted fluid as cell media or urine prior to EV isolation, allow easy removal of small molecules and ions from the EV preparation and can be used for EV dialysis and buffer exchange.


Offering fast EV purification from small (100 µl) to large volumes (up to 20 ml) of fluids in only 15 minutes, AMSBIO ExoUltra Size Exclusion Chromatography (SEC) columns are perfect for integration into almost any laboratory workflow. These stable columns can be cleaned and reused up to 5-times enabling EV purity improvement in applications including EV isolation from cell media, biofluids and plant extracts, purification of EVs from contaminated fluids and removal of excess dye post EV labelling process. Using a combination of AMSBIO TFF Filters and ExoUltra SEC columns, researchers will be able to separate subtypes of EVs based on their size thereby eliminating non-EV components.


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Effi cient Premium Fine Grinding


Fritsch’s Disk Mill PULVERISETTE 13 premium line grinds hard-brittle to medium-hard solids down to 0,05 mm fi neness. It is ideal for medium particle sizes in the areas of mining and metallurgy, ceramics industry, rocks and soils, glass industry, soil research.


The instrument is especially safe due to the automatic locking of collecting vessel and grinding chamber. And thanks to the simple motor-driven grinding gap adjustment with digital gap display and the presentation of all parameters on the well-designed display are absolutely convenient to operate.


The extra powerful motor provides high throughput of up to 150 kg/h. Fast, residue-free cleaning is ensured due to the smooth grinding chamber, inner surfaces, removable funnel and labyrinth sealing of grinding chamber. This Clean Design also protects against sample contamination.


The grinding disks can change the rotational direction (clockwise/counter-clockwise) thereby extending the service life. Grindings disks are available in 4 different materials for optimal adaptation to your sample material. The connection of a dust exhaust system, directly controlled via the instrument, enables dust-free grinding.


Now the Fritsch Disk Mill P-13 premium line can be combined with the high-performance Cyclone separator. This not only simplifi es fi lling and cleaning considerably, but at the same time fi ner gap widths for higher fi nal fi neness can be used without any problems. The strong airfl ow of the Cyclone separator accelerates the


throughput, increases the discharge from the grinding chamber and keeps the thermal load of the samples low, even with materials such as coal or chalk, which are otherwise diffi cult to grind fi nely. At the same time, the output of the sample can be actively controlled, and the fi ne dust load in the vicinity of the mill remains signifi cantly lower. The comminuted sample is fed directly into a screw-on glass and no longer has to be laboriously transferred manually from the drawer.


Watch the video: ilmt.co/PL/lr4D More information online: ilmt.co/PL/2WMJ


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Scalable Solution for Cost-effective AAV Production


The Gibco CTS AAV-MAX Helper-Free AAV Production System from Thermo Fisher Scientific is a new all-in-one solution designed to help meet clinical and commercial demands for cost-effective and scalable development of adeno-associated virus (AAV)-based gene therapies. To date, it is the only product of its kind that is manufactured under cGMP conditions to enable large-scale applications.


AAV is used in 82% of viral vector-based gene therapies in the pipeline, and scaled production is critical to accelerate the transition from research to commercialisation to bring gene therapies to market faster and at lower costs. By delivering high titre AAV production, the system can save manufacturers up to 50% on production costs compared to alternative polyethyleneimine (PEI)-based suspension systems and help reduce plasmid DNA costs by 25%. It features a mammalian-based suspension system comprised of a clonal HEK293 cell line and animal origin-free reagents that support scalable AAV production from shake flask to bioreactor scale.


“Building on 60 years of trusted Gibco solutions, the CTS AAV-MAX System is the latest addition to our fit-for-purpose line of scalable solutions for commercial manufacturing of cell and gene therapy applications,” said Amy Butler, President, Biosciences, Thermo Fisher Scientific. “More efficient, cost-effective AAV production is an essential step to streamline gene therapy development, helping drive innovation to serve those who need it most - patients who can benefit from these potentially life-changing therapies.”


As part of Cell Therapy Systems (CTS) solutions, the CTS AAV-MAX System is designed to work within the AAV production workflow, from cell culture and transfection through AAV viral vector production. Researchers can transition seamlessly from research on the Gibco AAV-MAX Helper Free AAV Production System to clinical scale using the CTS AAV-MAX System. Thermo Fisher’s CTS products are cGMP manufactured and include traceability and regulatory documentation, and are regularly used in commercial production to address cell and gene therapy developers’ manufacturing needs.


More information online: ilmt.co/PL/XABZ 59259pr@reply-direct.com


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