45 Sample Preparation & Processing
The Unbeaten Standard for Reproducible Sample Homogenisation
Nothing matches the performance of the original. Retsch’s Ultra Centrifugal Mills have set standards in sample homogenisation foralmost 50 years and are used by quality control laboratories worldwide.
The latest model ZM 300 comprises the essence of German engineering expertise combined with high-quality materials, smart accessories, and latest software technology. The powerful mill provides maximum grinding performance with an uneaten speed of 23,000 rpm. Classic centrifugal mills are usually limited to 18,000 rpm. At the same time, the ZM 300 was designed with operator convenience and safety in mind.
Grinding results are convincing: The ZM 300 produces particles which are 15 - 20% finer in comparison to conventional rotor mills, depending on the material. The high speed also allows for an increase of sample throughput by 10 - 15%.
The variable speed range allows for optimum adaptation of the grinding process to the sample requirements by keeping the grinding time as short as possible and the temperature increase as moderate as possible. Too much heat may have a negative effect on the grinding results, for example, if moisture or volatile components escape.
The ZM 300 is equipped with an integrated temperature sensor which measures the temperature of the cassette lid near the ring sieve. The measured temperature is constantly shown in the mill’s display, allowing the user to optimise the grinding process and improve reproducibility.
More information online:
ilmt.co/PL/p1vw and
ilmt.co/PL/z1xz 59552pr@reply-direct.com
Lysis and Hypotonic Sonication Buffers for ChIP Assays
Offering complete disruption of cytoplasmic and nuclear membranes, Porvair Sciences Chromatrap® buffer chemistries are optimised for sonication and enzymatic shearing.
The success of a ChIP assay is highly dependent on the quality of chromatin prepared. Chromatrap® lysis and hypotonic buffers are high performance reagents you can trust in this critical first step in isolating chromatin from your sample. For enzymatic shearing from fixed cells or tissue - both these detergent-based buffers are ideal for effective fragmentation of nuclear material.
For the disruption of cell membranes, Porvair Sciences optimised Chromatrap® hypotonic buffer composition ensures effective lysis of cell membranes to aid in the release of nuclei material from a wide range of varied species.
Formulated for difficult to lyse cell types and tissues, Porvair Sciences optimised Chromatrap® lysis buffer is proven to enable researchers to better explore and understand the underlying processes and mechanisms that drive biological organisms.
More information online:
ilmt.co/PL/6qGQ 59391pr@reply-direct.com
Streamlining Contaminant Testing of Medical Marijuana
Talon Analytical, a cannabis testing laboratory in Long Island, New York, is using Integra Biosciences’ ASSIST PLUS pipetting robot with 8 channel VIAFLO electronic pipettes to aid contaminant testing of hemp, CBD, and medical cannabis products. The instruments are integral to the company’s workflow, streamlining its testing process and ensuring robust and reliable processing.
The potency of medical marijuana products can vary significantly between manufacturers, and the cannabis plant itself is a hyperaccumulator, absorbing and
retaining toxins such as heavy metals and pesticides from the soil in which it grows. Talon Analytical was founded in 2019 to perform routine testing of cannabis-based medical products for the numerous companies involved in medicinal marijuana production across the state. It uses the ASSIST PLUS pipetting robot in combination with 1250 μl and 300 μl 8 channel VIAFLO electronic pipettes to provide precise dilution of extracted samples for HPLC analysis.
The company chose the ASSIST PLUS to streamline its workflow and ensure robust and reliable sample dilutions. Brynn Vitrano-Stocker, Quality Manager at Talon Analytical, explained: “Previously, we carried out all our dilutions by hand, which was prone to manual error and extremely time consuming. Now we have the ASSIST PLUS pipetting robot, our entire pipetting takes only eight minutes, which means we can process more samples every day and keep up with the increasing demand for testing.”
Chelsea Bissondayal, Head Analyst, added: “The ASSIST PLUS and VIAFLO electronic pipettes consistently lead to the highly precise and accurate results that manufacturers and the government require. This means that we don’t have to waste time or materials repeating tests. The instruments also significantly speed up our workflow and improve the lab’s overall throughput.”
More information online:
ilmt.co/PL/LaE3 and
ilmt.co/PL/O9lj 59392pr@reply-direct.com
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