39
Table 3. Lists the phosphopeptides detected by MS in the enriched peptide mixture from the cell division protein kinase 3.
Figure 2 Summary
Phosphorylation is pivotal in cellular regulation and hence one of the mechanisms responsible for disease progression for example in cancer. The cellular signalling proteins and pathways involved need to be identified and understood however there are many challenges to phosphopeptide measurement. Phosphoproteins are generally present in unsuitable forms and in such low concentrations that methods for their enrichment are required to enable routine detection by MS analysis. These studies clearly demonstrate that TiO2 Mag Sepharose magnetic beads are ideal for selectively enriching phosphopeptide concentrations in both simple and complex cell samples. Their strong affinity for phosphoproteins and physical ease-of-use make them a convenient tool for studying phosphorylation patterns.
*Acknowledgement
These data were kindly provided by Sara Lind PhD, Lu Lu, Lioudmila Elfineh and Prof. Ulf Pettersson, Department of Genetics and Pathology, Uppsala University; and Konstantin Artemenko PhD, and Prof. Roman Zubarev, Department of Cell and Molecular Biology, Molecular Biometry Group, Uppsala University.
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