23 Ensuring Your Microtitre Plates are Cold Enough on Ice
Used as an interface between a microplate and crushed ice, the BioCision CoolSink thermo-conductive plate holder provides fast equilibration of all wells to below 4ºC and excellent well-to-well temperature uniformity.
Many experimental and clinical applications require low temperature control for culture plates. However, a new Application Note published in BioTechniques demonstrates that although it is commonly assumed that a plate in direct contact with crushed ice will rapidly equilibrate below 4ºC, this is not the case. In fact, the unstable nature of crushed ice and insulating air gaps between plate and ice mean that uniform and reproducible cooling are very difficult to achieve. Brian Schryver and Rolf Ehrhardt, MD, PhD found that for 96-well plates placed directly onto crushed ice, the average well temperature was 6.7ºC, significantly above the critical threshold of 4ºC. Furthermore, the well-to-well temperature variation was as wide as 3ºC.
By contrast, placing a plate on BioCision’s thermo-conductive CoolSink and then putting the assembly on ice brings all wells to or below 4oC and the well-to- well temperature range was more uniform. The addition of an aqueous conductive medium (ACM) to replace air between the plate and CoolSink surface further enhanced results, with even faster equilibration to a final temperature of 3.3ºC, and less than 1ºC variation between wells.
According to co-author Brian Schryver, BioCision Vice President, Research and Product Development: “CoolSink’s precision-engineered “direct contact” stage design eliminates the insulating air gap typically found between plate wells and the resting surface. The module’s excellent thermo-conductivity, due to its patented novel alloy construction, gives fast equilibration to below 4ºC, and using an ACM produces near identical well temperatures. This set-up provides a very simple way for researchers to ensure that all their samples are treated uniformly with no ‘plate edge effect’. CoolSink is an ideal tool for increasing the reproducibility of results in a variety of applications, such as cell-based assays, ELISA, FACS staining and analysis, migration or adhesion studies, temperature shift assays or any work with temperature-sensitive biomarkers.”
Circle no. 76
Broad Range of Laboratory Refrigerators, Freezers and Environmental Chambers
Laboratory refrigerators, laboratory freezers and environmental chambers in sizes from under-the-counter to triple-door standing models are now available from Tovatech, based here. Manufactured by Nor-lake, these laboratory units are used for blood storage, plasma freezing, pharmaceutical refrigeration, stability testing and laboratory incubation chambers. “Norlake refrigerators and freezers used for blood bank and plasma applications lock for security and meet all applicable industry standards,” said Tovatech Director Dr Rachel Kohn. “All models, regardless of capacity, carry premium features necessary for critical blood storage.” Stringent requirements for drug stability testing or BOD applications are fulfilled with Nor-lake stability chambers for pharmaceutical and biological laboratories. “The company’s incubators deliver the same premium temperature control provided in their refrigerators for biotechnology, tissue cultures and other microbiological studies,” Dr Kohn said. “Humidity control options are available on certain models.”
Flammable storage refrigerators, enzyme freezers, and very low temperature freezers offer features tailored specifically to the specialised requirements of this type of equipment. “Examples include locks, alarms, and manual defrost to eliminate automatic temperature cycling,” Dr Kohn said. “Chromatography refrigerators provide easy access for set-up and operations and their improved air-flow design delivers the fastest temperature recovery in the industry.” Routine and heavy-duty laboratory refrigerator units are designed to minimise the effect of door openings and changing loads. All units are environmentally responsible without CFCs and many are Energy Star qualified.
Circle no. 77 ADVERTORIAL Upright Freezer for Long Term Storage
GFL Deep Freezers provide an ideal solution for storing organic substances for an extended period of time. Upright Freezers with a capacity of 500 l operate with microprocessor technique (RS 232 port) and are available with two temperature ranges: for 0 to -40ºC or -50 to -85ºC. Equipped with three inside compartments with insulating doors, optional shelves can be installed.
Features: Maintenance-free, energy-saving cooling unit, up to 150mm all-around insulation. Magnetic door sealing system reliably prevents frosting of door seal. Interior explosion proof and completely made of stainless steel. Potential-free changeover contact. Multiple alarm messages. The limit values for alarms can be set individually by the user. Settable alarm delays. Large variety of accessories, special makes on request.
The GFL Deep Freezer programme includes 6 Upright Freezers having 3 different storage volumes (96 / 300 / 500 litres) and 12 Chest Freezers with 6 different volumes (30 to 500 litres).
Deep Freezers High-Performance!
Upright Freezer, 500 litres, with storage system and three additional shelves
GFL Gesellschaft für Labortechnik mbH was founded in 1967. Experience of every-day application in detail, continuous technical advances and an excellent quality approach have led to more than forty years of success of laboratory devices that meet the toughest demands on material, functioning and design.
The company seated in Burgwedel near Hanover / Germany has a tight-knit web of agencies and distributors. Users in the medical, scientific and industrial field benefit from the precision and reliability of GFL Deep Freezers, Shakers, Water Baths, Shaking Water Baths, Incubators and Water Stills in more than 150 countries worldwide.
GFL products comply with valid European standards, bear the CE mark, are maintenance-free and easy to operate. A vested quality demand in accordance with international standards is documented for all GFL laboratory products with the certification to DIN EN ISO 9001:2008.
Circle no. 78 Deep Freezers Shakers
GFL Deep Freezers are manufactured using only high-quality, stringently tested materials that are particularly suitable for ultra-low temperatures.
The GFL Deep Freezer programme includes 12 Chest and 6 Upright Freezers with capacities ranging from 30 to 500 litres volume, equipped with the latest safety features. Temperature ranges: 0 °C to -40 °C or -50 °C to -85 °C.
All models work energy-saving and trouble-free in continuous operation, are maintenance-free and have been approved with the CE mark and the German TÜV seal for „certified safety“ (GS). Practice-oriented range of accessories.
Alternative solution for lab professionals: Sub-counter Upright Freezers 6441 and 6481.
For storing frozen materials ready at hand at the work station — integrable into a laboratory bench. Cabinet volume 96 litres, 3 drawers on ball bearings. Temperature ranges: 0 °C to -40 °C or -50 °C to -85 °C.
Shaking Water Baths Water Baths Incubators Water Stills
GFL Gesellschaft für Labortechnik mbH · Schulze-Delitzsch-Strasse 4 · 30938 Burgwedel / GERMANY Phone +49 (0)5139 / 99 58 -0 · Fax +49 (0)5139 / 99 58 21 · E-Mail:
info@GFL.de ·
www.GFL.de
Circle no. 79
Spotlight
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