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Convenient Gas Chromatography Column Selection


CHROMATOGRAPHY


New Method for Dioxin Analysis in Animal Feed


Phenomenex, Inc announces the publication of a new method for the analysis of dioxins in animal feed and tissue using high- resolution gas chromatography with mass spec detection (HRGCMS). Demand for these analyses has grown significantly in recent weeks, since the discovery of dangerous levels of the cancer-causing chemicals in animal feed produced in Germany. The new method, developed in collaboration with Vista Analytical, also analyses dioxin-like compounds dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs), which are often monitored in conjunction with dioxins to give an overall toxic equivalent (TEQ) for a sample. Dioxins enter the environment and subsequently the food chain through combustion of organic materials in waste incineration. Recently 2,256 tons of fat, incorporated into feed products in Germany, were found to be highly contaminated, posing the threat of chain-reaction through the food supply. Several countries have now banned imports of animal products from Germany as a precautionary measure.


Thermo Fisher Scientific, Inc announced the release of a free GC Column Selector app for iPhone. The new app helps GC chromatographers select the ideal Thermo Scientific GC column for either existing or regulated methods based on their current configuration. It provides a quick cross-reference for Thermo Scientific TRACE columns to GC columns from other manufacturers. Customers can simply insert their existing column part number or select a current column from the supplied list and the intuitive app recommends the best TRACE GC Column for each application.


The Thermo Scientific GC Column Selector iPhone app puts details about a wide array of phases offered in the Thermo Scientific TraceGOLD GC column range at chromatographers’ fingertips. Furthermore, the convenient application enables point-of-use column selection and the added functionality of contact information for sales or technical enquiries.


Circle no. 20


Dual-Membrane Syringe Filters Provide Heavy-Duty Sample Clarification


“While dioxin contamination is generally on the decline due to aggressive regulatory action, we can still see accidents, such as the one in Germany,” explained Sky Countryman, Manager of applications and applied technology for Phenomenex. “Robust analytical testing procedures are still in demand for the determination of potential dioxin threats in both feed and animal fat.” A technical note on the new Phenomenex HRGC/MS method has just been published and can be accessed at: www.phenomenex.com/dioxin. The method was used to analyse non-organic chicken feed as well as fish from the Mississippi River and Great Lakes and shrimp from a variety of international sources. Vista Analytical Laboratory is the premier provider of high-resolution mass spectrometry analytical services. With experience, expertise and responsiveness unmatched in the environmental industry, Vista has built an international reputation for performing difficult trace level analyses reliably on a variety of matrices while meeting or exceeding the most rigorous regulatory requirements.


Circle no. 19


SEC-LS Dual Integrated Detector Dedicated to Proteins


Malvern Instruments has launched the Zetasizer µV with OmniFACE and OmniSEC, a new SEC-LS (size exclusion chromatography - light scattering) detector package dedicated to protein analysis.


The proven Zetasizer µV offers dual DLS (dynamic light scattering) and SLS (static light scattering) capabilities. It can be used as a stand-alone detector, analysing samples in a cuvette, and now as a fully featured modular chromatography detector. For protein applications, this makes the Zetasizer µV an ideal tool. Applications in stand-alone batch mode include the study of molecular size and formulation stability, and in SEC detector mode the measurement of molecular weight, determination of oligomeric state and detection and quantification of oligomers and aggregates. The absolute nature of the measurement means that no column calibration is required.


The Zetasizer µV is available as a complete chromatography system or can be added to existing SEC modules. Malvern’s OmniFACE provides the necessary connection that enables the Zetasizer µV to be linked into any third party SEC system and detectors, so that output signals can be used by the OmniSEC software for results calculation. Connecting the Zetasizer µV is simplicity itself. Using standard chromatography tubing it can be added in series with other detectors such as UV and RI. The laser stability and optical setup of the Zetasizer µV ensure excellent baseline stability and signal-to-noise for maximum sensitivity, while the 8µL flow cell minimises band broadening.


Circle no. 21


UHPLC Hardware Meets DryLab Software


Phenomenex, Inc introduces the Phenex™-GF/NY dual- membrane syringe filter for viscous, particulate-laden samples. The new filter incorporates a glass layer, through which the sample passes first for elimination of heavy particulates, increasing the effectiveness of the second, nylon fiber membrane.


By removing particulates from the sample, the syringe filter prevents buildup and blockage of chromatography columns and frits while minimising wear and tear on injection valves. Proper sample filtration can ultimately reduce system downtime and component replacement.


Ideal for any applications with highly viscous or heavily particulate-laden samples, such as foods, biological fluids, soil and fermentation broths, Phenex-GF/NY performs at higher capacity than nylon-only filters, and requires less hand pressure to filter the sample. Phenex syringe filters are optimised for superior flow rates and high throughput. Target applications include food and beverage and environmental testing.


“These new Phenex filters are part of our growing line of chromatography accessories,” commented J.T. Presley, Brand Manager for Phenomenex. “They offer a convenient and inexpensive way to clarify samples for peace of mind, extended column and system lifetime and improved analytical results.”


Circle no. 23


Shimadzu cooperates with the Berlin/Germany based Molnár- Institute in applying the new DryLab 2010 software and the new generation of Shimadzu UHPLC instruments. QbD automated HPLC Method Development using DryLab 2010 enables the transfer of HPLC methods into most-modern Shimadzu UHPLC technology. By remaining in the design space, much higher flexibility is achieved in adjusting working conditions according to individual needs. DryLab 2010 and Shimadzu’s LabSolutions software complement each other perfectly. LabSolutions provides a platform for all of Shimadzu’s chromatographic systems. Even 3D detection technologies are covered. DryLab 2010 targets method development and applications to run under regulated environment with a special focus on the pharmaceutical industry. The software’s reliability rate is 99.8%. More than 106 experiments can be modelled based on a set of 12 basic experiments under pre-defined conditions.


DryLab 2010 now enables simultaneous optimisation of several measured experimental parameters (tG, T, pH, ternary composition of eluent B, buffer or additive concentration) with 9 other derived factors (column length, -ID, particle size, flowrate, %B(start), %B(end), gradient steps, dwell volume, extra column volume) to further define the Design Space of a separation. The 3D Cube is calculated based on 12 experiments using 3 gradient time-temperature (tG-T) models with one additional factor and it represents over one million modelled chromatograms and evaluates multiple parameters simultaneously. DryLab is useful in almost all chromatography applications in the lab. The software helps to develop complete methods in minimal time, shorten run times, find the best separation conditions for any component in a mixture and transfer methods to better and more modern instruments such as Shimadzu’s Nexera. FDA, ICH and other regulatory authorities promote and request application of QbD principles to simplify the exchange of complex information on chromatographic selectivity and critical resolution values, so as to support better method control including method transfer. Furthermore, the ICH Q8 has made a clear movement towards more flexibility in supporting development of new products.


Circle no. 22 Engineering Nanoparticle Coatings


Researchers working at NanoQAM, the nanotechnology research centre of the University of Quebec, are successfully innovating novel polymer coatings on the basis of data measured using a Viscotek high temperature gel permeation chromatography (HT-GPC) system from Malvern Instruments. The system is proving crucial for efficient polymer characterisation, reliably providing absolute molecular weight and structural information to support research goals. A dependable high temperature solution is a pre-requisite for the polymers under test, as it is for bulk polyolefins. Malvern has developed the Viscotek HT-GPC system specifically for polyolefin analysis. These polymers are only sparingly soluble, necessitating the use of high temperatures throughout the analytical cycle. The Viscotek HT-GPC provides a reliable, cost-effective solution for operation at temperatures up to 160oC. The full triple detector configuration delivers absolute molecular weight without extrapolation or correction, molecular size (Rg and Rh), intrinsic viscosity and branching information in a single GPC/SEC experiment.


Circle no. 24


INTERNATIONAL LABMATE - MARCH 2011 - CHROMATOGRAPHY


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