51 Biotechnology & Immunology Single Cell Sequencing Service for Oncology and Immunology
An expanded offering for single cell sequencing services has been announced by Macrogen Europe. Clients will now have access to an enormous array of options for input material, DNA library preparation and analysis. It provides scientists with highly in-depth transcriptomic data that is invaluable in areas such as immunology and oncology research.
Macrogen Europe’s offering includes the 10x Genomics Chromium 3’ single cell gene expression and the 5’ single cell immune profiling workflows. These workflows are widely used in single cell research and ensure excellent comparability with previously published studies. They also provide tremendous flexibility in terms of tissue types, cell numbers, and data output. Advanced data analysis pipelines are offered as an easy turnkey solution for intuitive visualisations of the sequencing data.
Immunology is a field of research where cell heterogeneity holds critical information on many important biological questions. Many populations of immune cells cannot be studied adequately when analysed as a single, uniform group. A single cell approach is needed to understand the underlying genomic mechanisms in many immunology-related research projects.
“Single cell sequencing is rapidly gaining popularity among genomics researchers,” said Dr Seungeun Lee, Technical Support Lead at Macrogen Europe. “Our upgraded service can help many scientists quickly start using this technology.” Macrogen Europe’s CEO, Dr Bongcho Kim, added: “We aim to remain a reliable outsourcing partner with a cutting-edge, versatile offering for our biotech and academic clients. This expansion of our portfolio is an important step in ensuring that we offer a broad portfolio of genomics services quickly and cost-effectively.”
Macrogen Europe has been offering fast, high-quality Sanger and next generation sequencing services since 2008. Using the latest DNA sequencing technology from industry-leading suppliers such as Illumina, Pacific Biosciences and Thermo Fisher Scientific, Macrogen Europe delivers accurate, reliable results to researchers and industry professionals across Europe and Africa.
More information online:
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New Study Reveals the Role of Heparan Sulfate in Obesity
AMSBIO reports how researchers at the Washington University School of Medicine have used their 10E4 Heparan Sulfate (HS) antibody in pioneering obesity research to quantify the role of HS in the process of intercellular mitochondria transfer to macrophages.
In recent published research, researchers from the Brestoff and Teitelbaum Labs demonstrated that adipose-tissue resident macrophages acquire mitochondria from adjacent adipocytes using HS. This process occurs in healthy conditions but is impaired in obesity. Further they have shown that genetic disruption of mitochondria uptake by macrophages reduces energy expenditure and increases diet-induced obesity in mice, indicating that intercellular mitochondria transfer to macrophages mediates systemic metabolic homeostasis.
Head of the group, Professor Jonathan Brestoff commented: “Mitochondria are the power plants of cells, and it has long been assumed that they are made in one cell and never leave. We discovered that is not really the case and found that fat cells give some of their mitochondria to an immune cell type called macrophages. In obesity, this transferring of mitochondria between cells goes awry, contributing to faster weight gain and worse metabolism. Using a tool called CRISPR, we screened the entire genome and figured out that cells trade mitochondria using a special type of sugar called heparan sulfate, which we think acts like a loading dock for receiving cargo like mitochondria. When we delete heparan sulfates on macrophages, mice get fat. This suggests to us that it is probably good for cells to trade mitochondria with each other. Our team is now trying to figure out how this mysterious and surprising process of mitochondria transfer works because we believe we can harness this biology to treat some human diseases.”
Dr Wentong Jia, a postdoctoral fellow at the Brestoff lab added: “The cell surface expression of heparan sulfate, a glycosaminoglycan required for mitochondria uptake in macrophages, depends on a key glycosyltransferase named EXT1. The 10E4 antibody from AMSBIO has helped us verify that we’ve successfully prevented Heparan Sulfate from being synthesised in cells that lack EXT1.”
“I find it fascinating that cells use heparan sulfates to take up mitochondria,” said Rocky Giwa, a PhD candidate in the Brestoff Lab. “I wonder if there’s a correlation between the amount or composition of heparan sulfates and a cell’s ability to efficiently take up mitochondria from other cells. Since the various HS antibodies have unique specificities, the different clones can help us start to attack that question.”
AMSBIO offers a comprehensive range of high quality Heparan Sulfate (HS) antibodies from F69-3G10, F58-10E4 and JM403 clones, which are ideal for targeted binding of HS in Heparan Sulfate Proteoglycans (HSPG) research.
More information online:
ilmt.co/PL/1pJM 57399pr@reply-direct.com
ADVERTORIAL New Shaker-incubator with Advanced Cooling Capability
The ES-20/80C shaker-incubator from Biosan for biotechnological and pharmaceutical laboratories is a professional category equipment. The typical applications include – microbial and cell culture cultivation, protein expression, solubility studies, general mixing, as well as other various applications in the fields of biology and chemistry. The unit is equipped with a newly developed triple eccentric mechanism for platform motion that provides supreme balancing characteristics, superior reliability and quiet operation. The achieved stability of the unit during vigorous mixing allows for stacking installation of up to 3 units which enables to save space.
The new display and easy to use user interface provide a clear and intuitive control of parameters and allow data logging, storage and display over time. Additional features, such as out of balance sensor and automatic thermostat failure detection make this shaker-incubator an advanced and safe product. Bluetooth connectivity to PC allows for data management, data logging, parameter control and profiling in a dedicated software that can be requested separately.
A combination of an electric heating element and a built-in heat-resistant brushless fan provide precise temperature distribution inside the chamber up to +80°C. Cooling of the chamber is provided by additional Peltier modules allowing to cool until 12.5°C below the ambient temperature. Additionally, an excellent sample temperature uniformity of ± 0.2°C at 37°C is achieved. The inner chamber is made of stainless steel. State-of-the-art motor, thermal insulation materials and parameter PID-control decrease the energy consumption and make the shaker- incubator highly energy efficient despite its relatively large size.
Available accessories range from dedicated platforms for different capacity flasks to universal platform with optional fixation clamps, test tube racks and sticky mats.
To learn more visit Biosan on stand B1.110 at analytica 2022. More information online:
ilmt.co/PL/24VE
51533pr@reply-direct.com
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