46 Streamlined Sample Pooling for Arbovirus Testing
The ASSIST PLUS pipetting robot from Integra Biosciences is helping the Arbovirus Testing Lab at the New Hampshire Public Health Laboratories in Concord, USA, to streamline sample pooling for West Nile and Eastern equine encephalitis virus testing. Laboratory supervisor Denise Bolton explained: “We adopted pooled testing in summer 2020, initially constructing the sample pools manually. However, the additional step complicated the workflow, required high levels of concentration and focus, and occupied over an hour of analyst time. Adding a manual pooling step was also quite risky from a quality standpoint, and we worried about the opportunity for analyst error.”
“We were already familiar with the ASSIST PLUS system, as we use it for our COVID-19 samples. It was easy to write a pooling program using the VIALAB software, and the flexibility of the 8 channel VOYAGER adjustable tip spacing pipette meant that there was no need to change our mosquito processing method. We use the platform to transfer aliquots from 2 ml tubes into a 96 well plate to create the sample pools, then add lysis buffer directly, using the entire pool for nucleic acid extraction. This streamlines the process, saving time and reducing the use of plastic labware, since we aren’t constructing high volume pools then transferring an aliquot for extraction. We have seen a 75% saving in extraction and PCR reagents, and reduced the use of PCR instrumentation for the surveillance program, as well as saving precious analyst time. Most importantly, we have confidence that the most complex step in the process is being performed without errors.”
More information online:
ilmt.co/PL/6zZW 57545pr@reply-direct.com
Cutting Mill Solutions for Every Application
Both models of the Universal Cutting Mills from Fritsch are ideal for size-reduction for a wide range of diverse materials due to variable adjustment of the rotational speed of the rotor, different rotors with various knife geometries, replaceable blades, practical sieve cassettes and that with an unmatched ease of cleaning. The wear- free, stainless steel labyrinth seals offers effective contamination protection and no additional heat effect for your samples.
The high-speed Universal Cutting Mill PULVERISETTE 19 comminutes up to 60 l/h of soft to medium-hard sample materials and fibrous materials at a torque of up to 30 Nm with reliable reproducible results. The variable rotational speed adjustment in increments of hundred between 300 and 3000 rpm enables fine tuning of the comminution process for each sample within a very wide range of applications.
The low-speed Universal Cutting Mill PULVERISETTE 19 with variable rotational speed adjustment between 50-700 rpm in increments of ten and a torque of up to 67 Nm enables a very powerful comminution of hard, tough-elastic samples and small sample quantities
The Universal Cutting Mill PULVERISETTE 19 is also available in both variable rotational speed versions in a corrosion-resistant stainless steel 316L version. All materials have an increased resistance to alkalis and acids and are conform to the guidelines of the food and pharmaceutical industry.
Combine your Fritsch Universal Cutting Mills PULVERISETTE 19 with a Fritsch Cyclone separator for sample exhaustion and an exhaust system. The strong airflow ensures simple feeding, increases throughput, reduces the thermal load of the samples and enables the use of finer sieve cassettes to achieve a higher final fineness.
The Fritsch high-performance, stainless steel Cyclone separator is particularly indispensable in the analytical sector and in the food and pharmaceutical industries, and for the processing of heterogeneous mixtures of material. Due to its high surface quality, it offers enhanced resistance to corrosive media such as alkalis and acids and is easy to clean.
For small sample quantities, the compact Fritsch small volume Cyclone separator can be completely dismantled and cleaned in a dishwasher for reliably preventing contaminations. Watch the video:
ilmt.co/PL/a9RW More information online:
ilmt.co/PL/QB1x
57496pr@reply-direct.com
Easy Vessel Switching Benefits Scalable Flow Chemistry System flow chemistry system.
A new report describes how AM Technology Ltd has used Asynt’s ReactoMate ATOM lab reactor support stand to enhance the speed and ease of switching reaction vessels feeding their Coflore®
Martin Monedero, Principal Chemist at AMT, commented: “Our Coflore flow systems are designed to be as versatile as possible, enabling users to optimise feed vessel set up according to the requirements of a particular reaction. For users to be able to swap reaction vessels quickly and easily is a significant benefit, hence deciding to integrate Asynt ReactoMate ATOM into our Coflore peripheral equipment solutions. It accommodates our range of existing 5, 10 and 20 L glass vessels from multiple suppliers without any issues and we have found the auto-align function for the overhead stirrer makes set-up so simple. The integral lifting/lowering mechanism allows easy one-handed adjustment of the position of the vessel which is a valuable feature for both safety reasons and user-friendliness. Consequently, we are now recommending that AMT customers integrate ReactoMate ATOM systems with our Coflore flow reactors to ensure that they get the best system possible.”
More information online:
ilmt.co/PL/2pmD 57143pr@reply-direct.com
High Yield Photochemical Flow Synthesis The Borealis™ Flow Photoreactor from Uniqsis sets a new benchmark for high yield photochemical flow synthesis.
Comprising a high intensity Borealis™ LED lamp unit and a Cold Coil™ standalone reactor module, the Borealis™ Flow Photoreactor brings flow photochemistry within the reach of almost any laboratory.
Fitted with a safety interlock, to prevent accidental exposure to high intensity light, the LED lamps (available in a range of fixed wavelengths - 370, 410, 440, 460 and 520nm), are powered by a programable power supply that automatically detects the wavelength of the Borealis™ LED module and adjusts the output characteristics accordingly. A temperature sensor and safety cut-out are fitted to protect the LEDs from overheating. An Inert gas purge input is provided for low temperature use.
To set up the Borealis™ Flow Photoreactor, a coil reactor is inserted inside a Cold Coil™ reactor module and clamped in place using the external adjuster. The Borealis™ LED lamp unit is then inserted into the coil reactor and connected to the programmable power supply.
The coil reactor temperature is controlled by connecting the Cold Coil to a either a cold-water supply (for reactions close to room temperature), or preferably to a high precision thermoregulation system.
More information online:
ilmt.co/PL/JBDe 57147pr@reply-direct.com
INTERNATIONAL LABMATE - APRIL 2022
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