Biotechnology & Immunology
Cutting-edge Genome Editing Services AMSBIO has expanded its range of custom gene editing services to complement its extensive portfolio of off-the-shelf CRISPR/Cas9 products.
A powerful technology, widely used in genome science, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 is a simple and efficient editing tool which allows for specific genome disruption and replacement facilitating the manipulation of specific genes with high specificity and low cell toxicity. CRISPR/Cas9 is a rapid technique that can be applied directly in embryo and is easily programmable by changing the guide sequence of the sgRNA to any sequence of interest. As a consequence, AMSBIO CRISPR/Cas9 products are widely used by research groups worldwide in applications including gene mutation studies, epigenetic editing, cell and embryo therapies, genomic-scale screening and many crop yield research.
AMSBIO’s range of custom services has been expanded to meet the exact requirements of your gene editing projects. The fast turnaround and affordability of these CRISPR/Cas9 services is designed to accelerate your genome editing research.
Specific and potent guide RNA (gRNA) is crucial for successful genome editing. The AMSBIO custom gRNA cloning service is a cost-effective and fast tool to accelerate your research. AMSBIO offer target sequence cloning into any of their wide range of CRISPR/Cas9 vectors with the options of customer designed or AMSBIO designed target sequences
These services also include the construction of custom donor vectors for genome editing applications including target sequence cloning into CRISPR/Cas9 vector, donor vector construction with a predesigned cassette or a donor vector of your own design.
In addition, AMSBIO offer a CRISPR editing service for cell lines and a service to design and produce custom knock-out, knock-in or point mutation CRISPR kits, which provides you with everything you need for your specific genome editing research in a single simple kit. More information online:
ilmt.co/PL/LqQw
56926pr@reply-direct.com
New Multifunctional Laboratory Centrifuge
Biosan presents a new modern benchtop, low speed laboratory centrifuge LMC-56 designed for convenient sedimentation, centrifugation and collection of necessary samples. It provides operation with tubes, blood collection systems, gel cards, microtest and ELISA plates.
Biosan’s centrifuges are designed for safe work (metal protecting housing), easy maintenance and wide application range in medical, biochemical, chemical, industrial and other type of laboratories.
Features include: • User-friendly centrifugation parameter input and simultaneous display of the set and actual parameter values; • Safe assay performance: metal protective housing and metal lid, automatic imbalance switch-off, lid lock during the centrifuge operation provide safe operation at all speeds; • Rotor imbalance automatic diagnostics (emergency stop, imbalance indication); • Automatic rotor detection with active rotational speed limit; • Relatively high speed - maximum 6,000 rpm or 3,160 g; • A wide selection of rotors - up to 13; • Improved chamber to reduce sample heating during centrifugation; • Wide choice of accessory rotors and adapters; • Rotor mode selection; • Different modes of acceleration and deceleration, including deceleration mode with switched off forced braking; • Possibility to set the speed both in revolutions per minute and by relative centrifugal force.
To learn more, visit Biosan on stand B1.110 at analytica 2022. More information online:
ilmt.co/PL/rPXE
55931@reply-direct.com
Effective Pipetting for Bacteriocin Testing Workfl ows
Integra’s VOYAGER adjustable tip spacing pipette is streamlining workflows for researchers at NOMAD Bioscience GmbH, a German R&D company specialising in plant biotechnology, in the preparation and dispensing of media and gels.
Research scientist Birgit Koch explained how she uses the VOYAGER in her work: “My research is focused on molecular cloning, to generate plant virus-based expression vectors that are transferred using agrobacteria into the host plants, and activity testing of the antimicrobial bacteriocins produced via transient transformation. The preparation and dispensing of samples, media and gels are everyday tasks in the lab, and this is where we benefit from the VOYAGER.
“The VOYAGER’s ability to automatically adjust the tip spacing at the press of a button was our main reason for choosing it. The electronic pipette allows the NOMAD team to efficiently pipette volumes between 5 and 125 µl for up to eight channels simultaneously, reducing the number of transfer steps required. Antimicrobial activity testing is where the VOYAGER has helped the most, to decrease the transfer steps. We do a ‘spot-on-lawn’ assay where we pipette drops of our peptide solutions onto bacteria grown on agar gel in square petri dishes (12 x 12 cm) and, if the bacteria are killed, then we know our bacteriocins are active. We also do it the other way around, pipetting bacteria onto an agar containing the bacteriocin, to see whether or not they grow. I used to work a lot with manual single and multichannel pipettes, and it would take me 40 minutes to finish a plate for an activity assay. Now, with the VOYAGER, I’m done within 10 minutes.”
More information online:
ilmt.co/PL/MBp2 57369pr@reply-direct.com
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