14 Enhanced Flowmeter for Liquid Chromatography Applications
Testa Analytical Solutions e.K has launched a new enhanced version of its popular Liquid Chromatography Flowmeter. Flow rate is one of the most important parameters in any liquid chromatography system, it determines retention time or volume and has by nature a major influence on reproducibility. The Testa Analytical liquid chromatography flowmeter has set a new standard for non-invasive measurement of flow rate in HPLC and GPC/SEC systems.
Version 1.2 of the liquid chromatography flowmeter allows users to now set the optimal integration time to suit different applications. For example, sampling data every 0.1 seconds is optimal for real-time monitoring of HPLC pump performance whereas sampling data every 10 seconds is best for absolute flow rate calibration.
The new version of the liquid chromatography flowmeter also offers the possibility for users to store on-board different calibration factors for different solvents, along with saving a clear text name of the solvent in use. Furthermore, improved communications data buffering assures that no data is lost even if the device temporarily loses connection to your PC.
Compact in size, and conveniently powered via a USB connection, the Testa Analytical liquid chromatography flowmeter uses a thermal flow sensor that is not only extremely accurate, sensitive and high-resolution, but it also offers the advantage of being non-invasive. As the sensor does not interfere with the measurement it can operate over a wide dynamic range, and has been demonstrated to be the perfect real-time flow monitoring tool for liquid chromatography applications including calibrating the flow rate of HPLC pumps, troubleshooting defective systems, and continuously validation for regulated products.
More information online:
ilmt.co/PL/zRLl 57174pr@reply-direct.com
Multi-angle Light Scattering Detector for Accurate Molecular Measurement
Over the past four decades, light scattering has become widely used for molecular weight and size measurement of proteins and polymers. However, the most recent and ever more demanding characterisation needs require to overcome the limitations of the various traditional technologies in light scattering detection. The Tosoh LenS3®
Multi-Angle Light Scattering (MALS) detector features an elongated flow path geometry that maximises scattered light collection, while
the non-refractive material of the chamber eliminates noise from stray light. The advanced optical design also provides exceptional signal-to-noise at the crucial extreme angles for molecular weight and size measurements.
The resulting enhanced capabilities and unprecedented sensitivity of the LenS3 MALS detector provide a direct and accurate measurement of molecular weight, even for oligomers and low dn/dc samples. Moreover, the range of radius of gyration (Rg) determination by light scattering is extended down to a few nanometers for the very first time.
Read Tosoh Bioscience’s latest whitepaper here:
ilmt.co/PL/0ddQ A new technical note delivers also more detailed information on the MALS detector:
ilmt.co/PL/1ddW More information online:
ilmt.co/PL/yJJo
55216pr@reply-direct.com
New Glass Coated Well Plates
High-throughput, automated laboratory workflows demand the highest quality consumables to reduce instrument downtime and minimise the need for repeat analyses. CRS Pure-Pass Well Plates and Sealing Mats are designed and manufactured to stringent quality standards to minimise contaminants that can affect assay results. They conform to dimensional compliance with ANSI/SLAS to ensure compatibility with a wide range of automated equipment. CRS well plates are manufactured from a high clarity, high purity polypropylene copolymer with extremely low levels of volatiles and extractables that is molded in a cleanroom and lot tested. The result is clear with a low extractable profile in common solvents like methanol and acetonitrile.
Pure-Pass glass coated plates receive a glass barrier coating that is applied in an environmentally friendly process. The glass barrier eliminates sample contact with the polypropylene substrate and offers a cost-effective replacement for glass vial inserts. The barrier is resistant to solvents which do not swell polypropylene. The glass coating also offers a more hydrophilic surface compared to the polypropylene surface, and this altered wetting may significantly reduce adsorption losses for some types of hydrophobic analytes and biomolecules. For more information on CRS glass coating technology, visit our blog post ‘Alternatives to Glass Vials?’. Contact CRS to discuss your application needs and see how their plates stack up to popular competitors in the market; or perhaps you’ve heard enough and are ready to ship.
More information online:
ilmt.co/PL/EkPJ 56333pr@reply-direct.com
UHPLC analysis of cyclic peptides under LC/MS compatible conditions
Cyclic peptides are characterised by polypeptide chains that are arranged in a cyclic ring structure. This structure offers various advantages over their linear counterparts: on the one hand cyclic peptides show a higher thermal stability and a higher resistance to digestion. On the other hand, their rigid structure results in a better biological activity since the binding to the target molecule is improved. All these characteristics turn them into highly valuable drug candidates.
Cyclic peptides have various applications; the peptides in this application note, polymyxin B sulphate, daptomycin and bacitracin are used as antibiotics for the treatment of bacterial infections, whereas cyclosporin A is used as an immunosuppressive agent. Other cyclic peptides can also have antifungal or antitumor functionalities.
In YMC’s application note ‘UHPLC separation using column of low hydrophobicity’, the UHPLC analysis of four cyclic peptides is shown. Based on the hydrophobicity of the peptides, a YMC-Triart Bio C4 column was chosen for this application. Since a relatively high concentration of acetonitrile is needed for elution, a C4 modification is a good choice since it provides more acceptable retention times. Since a temperature of 70°C is required, YMC-Triart Bio C4 is a better column choice due to its high temperature stability. Sharp peaks are obtained with a mobile phase containing formic acid, which is commonly used for LC/MS analysis. YMC-Triart Bio C4 shows high sensitivity using these LC/MS compatible conditions.
More information online:
ilmt.co/PL/oXmB 57026pr@reply-direct.com
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