4 Intuitive GC Column Selection Tools
Thermo Fisher Scientific, Inc announced the availability of intuitive gas chromatography (GC) column selection tools in its 2010-2011 Thermo Scientific Chromatography Columns and Consumables catalogue. The ‘GC Column Selection’ pages within the new catalogue provide a number of tools to aid in selecting the most appropriate product from the Thermo Scientific TRACE™ family of GC columns for either existing or regulated methods.
The ‘Thermo Scientific TRACE Phase’ selection table lists details for the wide array of phases offered in the TraceGOLD™, TRACE and TracePLOT™ GC column ranges. The ‘GC Column Selection by Manufacturer’ table provides a quick cross-reference for TRACE columns to GC columns from other manufacturers. If an ASTM, NIOSH or US EPA method is being followed, then the ‘Column Selection by Method’ tables indicate the best Thermo Scientific product.
The GC Column Selection pages also offer hints and tips for method development selection in terms of column phase, internal diameter, film thickness and column length. Meeting such requirements, the Thermo Scientific TRACE family of GC Columns offers high temperature stability and exhibits low bleed and long lifetimes. From general purpose non-polar to polar columns, TraceGOLD, TRACE and TracePLOT columns provide excellent quality and performance, with guaranteed reproducibility.
Offering 540 pages of innovative and proven chromatography tools, including many novel and unique additions, the new catalog offers the broadest collection of products and techniques to meet all needs, from sample preparation to separation and analysis. This makes it a must-have reference for laboratories utilising any form of chromatography.
Circle no. 8 Protein Standards for Mass Spectrometry Analysis
Amsbio has announced a new service whereby it can generate high quality Mass Spectrometry (MS) standards for over 5,000 human proteins. Produced in human HEK293T cells and labelled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L- Lysine, the full-length proteins with appropriate post-translational modifications are the best choice for accurate identification and quantification of protein biomarkers.
Amsbio offers stable isotope-labelled full-length protein standards for both matrix- assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) Mass Spectrometers enabling researchers to identify the best SRM and MRM transitions through experimental data.
The MS standards commercialised by Amsbio allow researchers to spike at an early stage of sample processing enabling accurate quantification. Produced in human HEK293T cell line the protein MS standards have authentic post-translational modifications with greater than 90% stable isotope labelling incorporation efficiency. As a consequence researchers are able to gain higher data consistency than by using synthetic peptide internal standards.
Circle no. 9
CHROMATOGRAPHY & SPECTROSCOPY
Microfluidics System Used in Breakthrough Breast Milk Study
Agilent Technologies, Inc announced that a group of researchers from the University of California, Davis, has made a significant discovery: human breast milk contains an unexpected abundance of sugars that coats the lining of infants’ intestines, protecting it from noxious bacteria. Results of the research, which used Agilent technology, were published in this month’s Proceedings of the National Academy of Sciences of the United States of America.
Agilent’s high-performance liquid chromatography polymer chip (HPLC-Chip) and quadrupole time-of-flight (QTOF) LC/MS technology provided researchers a new view of the oligosaccharide (sugar) structures produced in breast milk across stages of lactation among human mothers. Oligosaccharides are the third most abundant component in breast milk and prior to this study were thought to have no biological significance. HPLC-Chip/MS technology has enabled researchers to identify more than 200 different human milk oligosaccharides structures, which researchers have discovered are an important factor in the healthy growth of infants.
“This type of research has been performed and duplicated many times in the past,” said Dr Carlito B. Lebrilla, co-author of the research. “However, the new technology developed by Agilent made it possible to identify and quantitate milk oligosaccharides providing new and important observations that could not be obtained in the past.
Agilent commercialised the HPLC-Chip/MS concept in 2005, combining nanoflow HPLC columns, connecting capillaries and a spray emitter into a re-useable, credit-card-size device. This allowed the advantages of high-sensitivity and low- sample consumption provided by nano LC/MS to be accessible to scientists without the troublesome setup involving microvalve, fittings and capillary tubing of conventional nano LC. The addition of high-resolution, accurate mass MS/MS information from Agilent’s 6500- Series Q-TOF LC/MS systems enabled researchers to confidently identify sugar structures.
Circle no. 10
Standalone Ultrahigh-Pressure Pump for Proteomics Workflows Using LC Dionex Corporation is pleased to announce the expansion of its family of industry-leading Rapid Separation LC (RSLC) systems with the new UltiMate® 3000 NCP-3200RS nano/capillary LC pump
module. This new standalone ultrahigh-pressure pump features an extensive flow-pressure footprint, delivering continuous, direct flow rates from 20nL/min to 50 µL/min at backpressures of up to 800 bar (11,600 psi) for nano, capillary, and micro LC analyses.
The pump can be used to configure an entry-level RSLCnano system for reversed-phase separation of peptides in LC-MS proteomics workflows. Alternatively, the pump can be used as an add-on module to our industry-leading RSLCnano system for advanced applications, such as automated off-line 2D-LC or tandem nano LC. Tandem nano LC can be an attractive technique for customers who want to improve their mass spectrometry use and increase sample throughput. Dedicated application kits have been introduced for the RSLCnano system, such as automated off-line RP × RP of peptides and tandem nano LC, to facilitate instrument installation and get effective data from the first experiments.
In addition, Dionex has also released new biocompatible switching valves, featuring a maximum pressure specification of 345 bar (5,000 psi), for sample injection and column switching. These values are intended for use with the RSLCnano system, to provide a biocompatible sample flow path that can be important to customers dealing with the analysis of phosphopeptides.
Circle no. 11
HPLC in a Box
Anachem Instruments Ltd are proud to unveil the New Gilson PLC 2020, a personal liquid chromatography system for Reverse and Normal Phase HPLC with FLASH purification capabilities. This complete system was developed for chemists, to provide each with an easy-to-use, self-contained instrument specific to their purification needs on a more personal level. Ideally suited for low throughput, high and low pressure purification to support the individual or group of chemists.
The PLC 2020 is a compact and independent purification platform capable of performing manual injection purification runs from 1 to 100mL/min with pressures up to 4060 psi. The system can hold up to four 1 litre solvent reservoirs and select between four different elution solvents. It also has integrated variable, dual wavelength UV/VIS detection and fraction collection capabilities all controlled via the easy-to-use touch screen controller, allowing the system to be completely self contained and operated without the need for any additional equipment or space.
The PLC 2020 enables users to download/upload a variety of methods or results easily with a USB drive for quick information transfer. It’s interchangeable pump heads and flow cell design enables users to completely optimise and accommodate a variety of applications. This ‘HPLC in a box’ offers the diversity that chemists require with fully functioning facilities and an intuitive software interface.
Circle no. 12
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