10
Making an Informed Spectrophotometer Choice
CHROMATOGRAPHY & SPECTROSCOPY
New Grade of Acetonitrile for HPLC Applications
Mallinckrodt Baker, a business unit of Covidien, has introduced a new grade of acetonitrile to match the quality requirements for customers performing routine high-performance liquid chromatography (HPLC) applications and analysis. Mallinckrodt Baker’s new
J.T.Baker®
LC/MS applications.
J.T.Baker®
HPLC-9012 Acetonitrile rounds out the company’s offering of acetonitrile products specifically developed for HPLC and HPLC-9012 Acetonitrile is application-optimised and function-tested specifically for use in isocratic HPLC
(refractive index or UV/Vis) and routine gradient HPLC (UV/Vis) analysis, as well as spectrophotometry, ion chromatography, biopharmaceutical purification and quality control testing.
J.T.Baker® HPLC–9012 Acetonitrile is gradient elution tested at 254nm and meets ACS specifications for general use, liquid
chromatography suitability and UV spectrophotometry. In addition, Mallinckrodt Baker’s manufacturing expertise in purification, packaging and analytical testing ensures strict quality control to provide the lot-to-lot consistency required in HPLC methods and applications.
The new 9012 HPLC grade is intended to complement Mallinckrodt Baker’s existing
J.T.Baker® HPLC Ultra Gradient–9017 Acetonitrile, a
Selecting a spectrophotometer has never been easier as Biochrom launched its new user-friendly guided navigation as part of the company website, to help customers choose the ideal Biochrom spectrophotometer for their application.
The updated website includes details of the company’s comprehensive portfolio of high performance instruments, including Biochrom Libra UV-Vis spectrophotometers for industrial, analytical and pharmaceutical laboratories. Biochrom WPA colorimeters and spectrophotometers for education and the life sciences are also included in the knowledge-based selection tool. Visitors to the website will find extensive online information and a comprehensive technical glossary, all designed to inform instrument selection.
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higher purity grade of acetonitrile, which is designed for more advanced HPLC methods and applications such as trace concentration or environmental analysis, using evaporative light scattering or fluorescence detection. “In response to the recent worldwide shortage of HPLC acetonitrile, our expanded offering helps customers reduce their supply chain and quality risks, and also improve their ability to manage costs,” said Russell Thorpe, Global Marketing Director for Laboratory Products at Mallinckrodt Baker.
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Clean in Place Immersion Probes
Low-Power Source Offers High Pulse Rate
Quality Control of Drugs and Analysis of Unknown Proteins
Many industries rely on the principle of Clean in Place (CIP) as a method of maintaining the integrity of interior surfaces of pipes, but this has rarely been applied to optical immersion probes until now.
Shimadzu has introduced both PPSQ 30A and 33A Edman Sequencer models to the European market.
Edman degradation has been developed by Pehr Edman and is a longstanding established method whereby an amino acid is sequentially cleaved from the N-terminus of a protein, derivatised and separated via HPLC to determine its retention time. By comparing the retention times with those of standard amino acids, the N-terminal sequence can be determined.
Although in recent years various mass spectrometric methods have replaced Edman degradation for the identification of proteins, this technique is still very useful. More and more protein-based drugs are being developed, and particularly in these cases it is necessary to accurately determine the N-terminus for quality control.
In spite of longer analysis times, Edman degradation is reliable, robust and its results are easily interpretable. In addition, Edman degradation also enables unequivocal differentiation between isobaric amino acids such as isoleucine or leucine that have the same mass but different structure. Possible impurities can be more effectively determined and quantified via Edman degradation. Identification of proteins that are not included in databases can also be carried out very effectively via Edman degradation.
The PPSQ series operates under isocratic separation conditions leading to high reproducibilities. The operating costs are also clearly reduced as HPLC solvents can be recycled and the required reagents can be purchased all over Europe from WAKO Chemicals. Shimadzu’s extensive European service network is an additional reason for deciding to replace an existing system or to a acquire a new system.
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With immersion spectroscopy in the mid infrared becoming more prevalent in pharmaceutical production, Fibre Photonics can now offer the CIP functionality to its range
of ATR immersion probes in the spectral range 0.18-18µm (550cm-1 to 55500cm-1).
A range of Fibre Optic Spectroscopy (FOS) ATR immersion probe systems are now available with automated CIP. Cleaning is now faster, less labour intensive, more repeatable, and poses less of a chemical exposure risk to people. Maintaining the integrity of optical analysis methods is critical in applications such as: reaction monitoring; crystallisation development and screening; process analytical technology (PAT); analytical characterisation; and biopharmaceutical analysis.
Only an optical system with automated CIP can allow reliable analysis at any time during chemicaland biological processes. The semi or fully automated SensoGate-FOS and remotely operated Ceramat-FOS with UNICAL-FOS 9000 controller are designed such that fibre optic immersion probes can be cleaned and used with ease. Monitoring of the cleaning process with a detector/analyser is possible, and when the baseline is reached, drying starts and the probe is pushed back into the process measurement position.
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Miniature spectroscopy pioneer Ocean Optics has added a pulsed xenon light source option for its Jaz modular sensing system. The Jaz-PX is a high flash rate, short-arc xenon lamp especially useful for UV-Vis applications such as absorbance, bioreflectance, fluorescence and phosphorescence. The lamp has maximum pulse frequency of 500 Hz and spectral response from 190-1000nm.
The Jaz-PX operates in both free running and triggered modes, which allow its pulses to be coordinated with other devices in the Jaz stack. Flash-to-flash stability is <1% and the flash rate is 500 Hz. Jaz-PX has an SMA 905 connector that couples to Ocean Optics accessories, including optical fibres, cuvette holders, probes and other sampling optics. Because it produces a pulsed signal, the Jaz-PX is less likely to cause solarisation in optical fibre assemblies, which can occur when fibres are illuminated with signal <260nm.
In addition, Jaz can be configured for a variety of field, lab and process setups. The Jaz-PX is ideal for field applications such as bioreflectance that require a high-intensity, low-power source as no external power supply is required. When used in the field, the lamp will operate from the power of the battery for approximately 3.5-4 hours. Additional power is available through an optional 50 Watt-hour Jaz battery extender.
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