62 New Cryo-correlative Microscopy Stage Greatly Improves Workflows
Linkam has been developing cryo stages for correlative microscopy for many years and continues to be at the forefront of cryo correlative microscopy with the latest update of their LINK software for the CMS196M, providing improved imaging capabilities and the new liquid nitrogen autofill system providing longer run times.
Cryo-correlative microscopy has become an established technique in recent years. It brings together the strengths of both electron and light (fluorescence) microscopy. Electron microscopy (EM) provides structural information at very high resolution. However, it can give only restricted insight into biological and chemical processes due to limitations in staining and sample preparation processes. On the other hand, fluorescence microscopy is an extremely sensitive technique to detect biological, chemical and genetic processes as well as events inside living cells.
Cryo-CLEM brings it all together: combining the individual advantages from both fluorescence and EM by imaging the same sample location with both techniques and superimposing the complementary information.
For biological samples to be compatible with the vacuum conditions found in EM and preserve the structural detail, samples are embedded in vitrified ‘glass like’ ice and need to be kept below -140°C. Any contact with moisture contained in the air has to be avoided since ice crystals would form immediately and contaminate the sample. Under cryo-conditions, the fluorescence signals providing structural detail is preserved and photo bleaching is significantly reduced.
LINK software for the CMS196 provides complete control and monitoring of the system as well as fully-automated tiled image capture. The integrated, encoded, high resolution motorised XY stage gives the ability to capture an image of the entire EM grid at full resolution. Shown below, the system produces a single image of the EM grid that can then be used to navigate the sample and save co-ordinates of areas of interest making it easy to image the same area when the sample is transferred to the EM. In addition, LINK can now be supplied with an extremely cost effective, high sensitivity monochrome camera ideal for low light fluorescence imaging.
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High-Speed Camera Enables Video Recordings of Microscopic Events
Fastec Imaging’s IL5 High-Speed 5MP Camera is easily mounted on your microscope, enabling you to record high-speed video of microscopic events. Both spatial and temporal magnification work in tandem to clarify understanding in applications such as microfluidics, where particles often move through the field of view very quickly. With four models to choose from, boasting crisp, clean video from 2560 x 2080 @ 230fps to 800 x 600 @ 1650fps, there is an IL5 to fit your application needs. All models record over 3200 fps at VGA resolution and more than 29,000 fps at smaller resolutions. Able to save images to an SSD or SD card while recording high-speed bursts of hundreds or even thousands of images at a time, the IL5 is always ready for the next high-speed snapshot. With a base model starting below $9,000, the IL5 is a cost-effective solution to record high-speed events for slow motion analysis.
Built for flexibility and ease of use, the Fastec IL5 camera can be controlled over Gigabit Ethernet via Fastec FasMotion software on your PC/Mac or via the built-in web interface with your favourite web browser on your PC, Mac, tablet, or even your smartphone. Using the (LR) FasCorder Mode, operate the camera as a regular camcorder to record and pause as needed and follow the action, stop recording and review what you have, and then append additional footage at will, even after a power cycle. Unlike traditional high-speed camera systems that only record for a few seconds and require careful triggering, the IL5’s Long-Record (LR) option can record at high speed for many minutes at high resolutions, to many hours at reduced resolutions.
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New Class of Confocal Microscope Scans Large Areas at High-Speed
Caliber I.D. has launched the RS-G4, a new class of modular confocal microscope that delivers confocal’s expected high resolution and clean contrast while overcoming its limited scan areas. Scanning large areas at lightning fast speeds, RS-G4 dramatically cuts the time required for efficient study of next-generation large format samples including brain slices, plant cross-sections, or large fields of cells or tissue critical for high-throughput screening.
Consider the displayed image of a whole mouse brain. The RS-G4 scanned and stitched 1050 fields to image this 12mm x 8mm area (approximately 0.5” x 0.25”) in just 270 seconds (4.5 minutes), approximately one-tenth the time required by other currently-available systems. Yet, as demonstrated by the inset, the image retains all the confocal detail and resolution offered by the 40x/1.30NA oil immersion objective.
cornerstone of RS-G4’s innovative performance is its high-speed strip mosaic imaging. On the hardware side, continual stage movement is coordinated with the resonant scanning of the confocal microscope. Simultaneously, a proprietary stitching algorithm assembles and aligns mosaic strips with pixel level resolution. Hardware and software control are integrated under an easy-to-use, intuitive GUI. Adding a new laser launch opens opportunities for fluorescence excitation at 405nm, 488nm, 561nm, 640nm and, critical for deep tissue neuroscience, 785 nm. In addition, the RS-G4 can collect large area 3D image stacks that can be readily imported by any conventional processing package such as Image J or Imaris. Surprisingly, all of this capability comes in a small 18” x 15” footprint.
“We at Caliber are thrilled to be launching this revolutionary product (the RS-G4) into the research market,” said Robert Kelley, Caliber I.D.’s Vice President of Sales. “For researchers, this increased scan speed means greater productivity and shorter time to publication.”
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New Full HD Multi-Output Camera Launched
Motic Microscopes presents a new Full HD Multi-Output camera, the Moticam 1080, as the improved successor of the well-established model Moticam580. The Moticam 1080 is dedicated to documentation of microscopic results with a clear focus on maximum colour fidelity and fast live image. The Moticam 1080 as a stand-alone system works without computer and delivers an impressive 1080 (60P) live image on HDMI screen. Still images or videos from the microscope can be saved onto an SD card.
To control image and capture parameters via a powerful onboard software, just connect a wireless mouse to the USB port. In case the full functionality of the new Motic Images Plus 3.0 with its measuring tools is needed, the Moticam 1080 can also be plugged into a Windows, OSX or Linux system through USB. “With its powerful onboard software the new Moticam 1080 displays a state-of-the-art live image, a real highlight in the history of Motic’s Multi-Output cameras,” Dr Hans-Jürgen Klemenz, Product Manager in Motic Europe, pointed out. The Moticam 1080 comes along as a well- known All-in-one Box concept. Software and additional mounting options are included as well as a calibration tool.
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INTERNATIONAL LABMATE - APRIL 2017
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