41 Biotechnology & Immunology Acknowledgements The authors wish to thank Ben Brankatschk, Sabrina Galinski, Alexandra Rupp und Elena Ciirdaeva for their support, contributions and stimulating discussions. References


[1] Botvinnik, A., Wichert, S.P., Fischer T.M., Rossner, M. J. Integrated analysis of receptor activation and downstream signalling with EXTassays. Nat. Methods. 2010 Jan; 7(1): 74–80.


[2] Koboldt, D.C., Steinberg, K. M., Larson, D.E., Wilson, R.K., Mardis, E.R. The Next-Generation Sequencing Revolution and Its Impact on Genomics. Cell. 2013 Sep 26; 155(1): 27–38.


[3] Nitzki, F., Herbig, E. In situ Hybridisierung – Die Bedeutung von Reinstwasser für RNA Technologien. GIT Labor-Fachzeitschfi t. 2013 März; (57. Jahrgang, 3): 157–159 (also available in English: In Situ Hybridization: The Importance of Ultrapure Water for RNA Technologies).


[4] Rusk, N. Torrents of sequence. Nat. Meth. 2011 Jan; 8(1): 44–44.


[5] Patel, R. K., Jain, M. NGS QC Tookit: a toolkit for quality control of next generation sequencing data. PLos ONE. 2012; 7(2): e30619.


[6] Altschul S.F., Gish, W., Miller, W., Meyers, E. W., Lipman, D. J. Basic local alignment search tool. J Mol Biol. 1990 Oct 5; 215 (3):403–410


[7] R Core Team. A language and environment for statistical computing. R. Foundation for Statistical Computing, Vienna, Austria. 2012.


[8] Mei, L, Nave, K-A. Neuregulin-ERBB Signalling in the Nervous System and Neuropsychiatric Diseases. Neuron. 2014 Jul 2; 83(1): 27–49.


[9] Citri, A., Yarden, Y. EGF-ERBB signalling: towards the systems level. Nat. Rev Mol Cell Biol. 2006 Jul; 7(7): 505–516.


[10] Wehr, M. C., Laage, R., Bolz, U., Fischer, T. M., Grünewald, S., Scheek, S., et al. Monitoring regulated protein-protein interactions using split TEV. Nat. Methods. 2006 Dec; 3(12): 985–993.


[11] Life Technologies. Ion PGMTM Sequencing 200 Kit v2 [Internet]. 2013. Available from: https://www. lifetechnologies.com/order/catalog/product/4482006. Last retrieved on 15 April 2015.


[12] Avraham, R., Yarden, Y. Feedback regulation of EGFR signalling: decision making by early and delayed loops. Nat Rev Mol Cell Biol. 2011 Feb; 12(2): 104–117.


1Max Planck Institute for Experimental Medicine (Max-Planck-Institut für Experimentelle Medizin), Goettingen, Germany; now Systasy Bioscience GmbH, Munich, Germany


2Sartorius Lab Instruments GmbH & Co. KG, Goettingen, Germany


Cutting-Edge Cell Culture Technology on Show at Labvolution


This year’s Labvolution focuses again on the lab of the future. The Eppendorf booth, seminars and workshops are also fully dedicated to this subject. There will be tours of the glass laboratory, centred on the optimisation of work steps in the cell culture lab, offered directly at the booth. Eppendorf cell handling experts will provide tips and tricks for the proper handling of cells and put contamination avoidance under the spotlight. At the trade show, Eppendorf will also offer a seminar on this topic, where customers’ questions will be answered in detail.


There are also several new developments to discover when it comes to digitalisation and emerging technologies. This year Eppendorf has once again contributed to the design of the smartLAB and will address the aspects of these topics with products, presentations and a podium discussion. A corresponding workshop will also be held, giving customers the opportunity to discuss their concepts of and wishes for the lab of the future.


Even the Eppendorf booth itself is fully connected. The Eppendorf VisioNize system will be introduced to visitors at the world premiere. This system, which will be available soon, consists of software and hardware components which make it possible for customers to connect several Eppendorf devices in the lab. A central software component allows users to view the current status of the connected devices at any time and from virtually anywhere. This way, the temperature of a freezer, for example, can be displayed and recorded for documentation and analysis purposes. If configured limits are exceeded, the system informs the user with an email notification. An entirely new user interface, which ensures identical operation on the device and on the software, makes use and evaluations easy. VisioNize has made part of the future a reality!


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Fast and Convenient DNA Prep Kit Launched


Cole-Parmer, a leading manufacturer of laboratory equipment, has announced that it has launched the DNA Prep Kit by Arcis Biotechnology for fast and convenient DNA or RNA extraction. This new sample preparation kit allows rapid extraction of either DNA or RNA in three minutes with high yields, and is ideal for use with Cole-Parmer’s Delta Seek reagents and Techne or PCRmax PCR and qPCR hardware systems.


Existing sample preparation kits currently require laborious washing, heating and centrifugation steps that typically take 40 to 90 minutes to extract DNA or RNA from a sample. The Arcis DNA Prep Kit requires two simple steps that are completed in just three minutes, to provide high quality, PCR-ready templates with no need for additional laboratory equipment such as centrifuges or hot blocks. By simplifying the workflow while maintaining high yields, this product is ideal for multiple applications such as PCR (End-point, RT-qPCR), Real-Time PCR (qPCR), Isothermal amplification (LAMP), Sequencing (Sanger, Pyro), Genetic profiling (Electrophoresis) and forensic studies. The extraction system can also be easily integrated into automated processes.


The Arcis DNA Prep Kit, if used for extractions from fluids such as blood, requires as little as 30 µl of sample as a starting material and, unlike other products, can extract both DNA and RNA. This dual capability removes the need for separate DNA and RNA extraction kits and, combined with the unrivalled three-minute sample processing time, saves time and money for labs of all sizes.


“The Arcis DNA Prep Kit will massively reduce the time in which DNA and RNA samples can be obtained,” said Andrew Birnie, Business Development Manager at PCRmax. “It’s such a simple product that makes a usually laborious process easy and convenient, without compromising on yields. It’s ideal for any molecular biologist.”


If you are interested in testing the Arcis DNA Prep Kit, please contact Cole-Parmer as samples are available upon request. 42033pr@reply-direct.com


New Shigella Monoclonal Antibodies


Shigellosis is a foodborne gastrointestinal illness caused by several species of Shigella bacteria. Transmission is via the faecal-oral route and symptoms include cramps and diarrhoea which can progress to dysentery.


Shigella sonnei is the leading cause of disease in industrialised countries while Shigella flexneri is the most common species seen in developing countries; the predominate serotype being Shigella flexneri 2a.


ViroStat has recently released two sets of new Monoclonal Antibodies to Shigella: one set specific to Shigella sonnei and another set specific to Shigella flexneri 2a. Potential applications for these include direct bacterial detection by ELISA and lateral flow rapid tests.


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